LOCUS       sequence-369372-        2664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes TRP1 as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT032
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(174..762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(254..273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             944..1603
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1010..1029)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1674..1708
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1774..2448)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(join(2449..2664,1..62))
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cacatatatt acgatgctgt tctattaaat gcttcctata ttatatatat agtaatgtcg
       61 tttgtaatgt cttcacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgatt
      841 gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      901 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      961 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1021 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1081 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1141 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
     1201 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1261 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1321 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtttc
     1381 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1441 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1501 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     1561 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc
     1621 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     1681 cgctcggttg ccgccgggcg ttttttattg gtgaagacat actcgcaggc aagtgcacaa
     1741 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
     1801 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
     1861 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
     1921 cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca
     1981 atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg
     2041 tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat
     2101 aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac
     2161 gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa
     2221 cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta caagacttga
     2281 aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc
     2341 cagcaagtca gcatcggaat caagagcaca ttctgcggcc tctgtgctct gcaagccgca
     2401 aactttcacc aatggaccag aactacctgt gaaattaata acagacatac tccaagctgc
     2461 ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc ctccctcttg
     2521 gccctctcct tttctttttt cgaccgaatt gttaatcggc aaaaaaagaa aagctccgga
     2581 tcaagattgt acgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc
     2641 atctggcgtc ataactgcaa agta
//
