LOCUS       sequence-369373-        3283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes MET17 as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT033
FEATURES             Location/Qualifiers
     source          1..3283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(115..703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(195..214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             885..1544
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(951..970)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1615..1649
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1717..3051)
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
ORIGIN
        1 gcgtgtaatg tcttcacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
       61 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      541 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgat
      781 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
      841 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      901 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      961 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1021 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1081 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     1141 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1201 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1261 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt
     1321 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1381 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1441 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1501 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     1561 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1621 acgctcggtt gccgccgggc gttttttatt ggtgaagaca tactccttgt gagagaaagt
     1681 aggtttatac ataattttac aactcattac gcacactcat ggtttttggc cagcgaaaac
     1741 agtttcaaaa gattgctgga agtctgcaat aatgtcatca ataaattcga taccaacaga
     1801 gacacgaatt aagtccttgg taacaccaga tgccaacttt tctttgtcat ttaattgttt
     1861 gtgggtagtg aagtatggag caatgactaa ggtcttggca tcaccaacat tggccaagtt
     1921 agaggcaagc tttaaattgt caacaacttg agcaccagaa agtttgaatg ggtcagtttc
     1981 cttgtcggca tttggtaagt cttttacacc gaaagataag acaccaccga aaccgttaga
     2041 tagatacttc ttagcatttt catgatgaga atgagatgct aaaccagggt atgaaaccca
     2101 agatacgtat ggggattgtt ctaaccattt ggctaacttc aatgcatttt caccgtgtct
     2161 ttcagctctc aaagataatg tttcaacacc ttgtagtagc aagaaagagg caaatgggtt
     2221 catcaatgga cccaaatctc ttaatagttc agttctaaca tgaacgatgt atgccaagtt
     2281 accgtaggct tcattgtaga tagtaccgtg atatccttcg gcaggttgag agaattgagg
     2341 gaacttttct gggtagtcct tccatgggaa cttaccagag tcaacaataa taccaccgat
     2401 agtagtacca tgaccaccaa tccatttggt agcagaatgt gttacaatat cagcaccgta
     2461 tttaattggc tgacagaagt aaccaccggc accaaatgtg ttgtcaacga caactggaat
     2521 accgtgtttg tgagcaattg caacaatttt ttcaaaatcc ggaacattgt actttggatt
     2581 accaatggtt tccaaataaa cagccttggt tctttcatca aagacctttt caaattcttc
     2641 tggattgtca ccttcaacaa atctagcctc gataccaaat cttttgaacg agattttgaa
     2701 ctggttataa gtaccaccgt ataagtaaga agtggaaacg atgttgtcac cagtgtgtgc
     2761 caaaccttgg atggcaaggg tttgagcggc ttgaccggag gaaacagcca aagcagcagc
     2821 accaccttct aaagcagcaa ttctttcttc caaaacatta ctggttgggt tttggaaacg
     2881 ggaatagacg taacctggaa cttcaagacc aaacaattgc gaaccatgct tagagttttc
     2941 gaaaacataa gaagtggtgg cgtaaattgg tacagctctg gatctgtgag cattgtcacc
     3001 agggttctct tggccggcgt gtagttgaac agtatcgaaa tgagatggca ttgtatggat
     3061 gggggtaata gaattgtatc tatgtatctg acgaccctgt attacacgaa ggaaatagta
     3121 gatgaaaaga caagagagca agaaaaagga aaaacttcct ttctaacaga cgctttactt
     3181 aaccttatat atatcttatt tttttcatcg agcgtgttca attggacaag gtgccacctt
     3241 ttcgacactt cggttattat gttacacagt tttcatgagg atg
//
