LOCUS       sequence-369378-        2820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes NatR as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT036
FEATURES             Location/Qualifiers
     source          1..2820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1250..1838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1330..1349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2020..2679
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2086..2105)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2750..2784
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(join(2801..2820,1..178))
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga
       61 tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa
      121 tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtta
      181 ctttaagggc aaggcataga catatacaag gcctgttcac cgtcagatgc tgttccatca
      241 tataaagcag tatctaaacc acataatgtg aaacccattc ttctataagc atggatagca
      301 ggggcgttaa cattagtaac ttctaaccat aaatgaccgg ctcctctctc tcttgcaaat
      361 tctgtagcca aacccatcaa agctcttcct acaccatgac ctctatgctc tggggcgact
      421 tctatatctt caacggtcaa ccttctattc caacctgaat aagaaacgac tacaaaacca
      481 gccaaatcac cgtcatcacc gtatgctacg aaagttcttg aatctgggtc accatcttct
      541 ccgtcgtcgc tttcgtcatc agattcatca tctggaaaga ctttagttaa tggagggtcg
      601 actgggactt ctcttaaggt aaatccatca ccggtggcgg ttactctaaa gacggtgtcg
      661 gtggtgaatg aaccgtctaa agcctcaatt gcttcagcat caccagggac tgatgttctg
      721 tatctgtagg ctgtatcatc taaggtagta cccatttttg tttatgttcg gatgtgatgt
      781 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
      841 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
      901 tgaggggagc gtttccctgc tcgcaggtct ggagcgagga gccgtaattt ttgcttcgcg
      961 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
     1021 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
     1081 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaactg
     1141 taatgtcttc acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgattgatc
     1921 gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     1981 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2041 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2101 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     2161 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     2221 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
     2281 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     2341 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     2401 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtttcagcc
     2461 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     2521 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     2581 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     2641 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg
     2701 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     2761 cggttgccgc cgggcgtttt ttattggtga agacatactc cagtatagcg accagcattc
//
