LOCUS       sequence-369381-        2622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ZeoR as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT038
FEATURES             Location/Qualifiers
     source          1..2622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     358..377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1331..1528
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(1545..1579)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1650..2309)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2224..2243
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2491..2622,1..457)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
       61 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      541 ttctgtggat aaccgtgaag acattacagt ttagcttgcc tcgtccccgc cgggtcaccc
      601 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
      661 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
      721 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctccagac
      781 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
      841 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
      901 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaaa aatggctaaa
      961 ttaacatctg ccgttcctgt tttaacagct agggatgttg caggtgctgt agagttttgg
     1021 acagataggt taggattctc aagagacttt gttgaggacg attttgctgg tgttgtcagg
     1081 gatgacgtta ctttatttat ctcagcagtc caagatcaag ttgtccctga taatacattg
     1141 gcttgggtct gggtcagggg tttagatgaa ttatatgctg aatggtcaga agttgtatct
     1201 acaaacttca gagatgcttc tggtccagct atgaccgaga ttggtgaaca gccatggggt
     1261 agagaatttg ctttgagaga tccagctgga aattgtgttc attttgttgc tgaagaacaa
     1321 gattaaagta actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     1381 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     1441 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     1501 tcgtatgtga atgctggtcg ctatactgga gtatgtcttc accaataaaa aacgcccggc
     1561 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1621 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1681 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1741 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1801 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1861 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1921 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1981 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     2041 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     2101 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     2161 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     2221 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     2281 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     2341 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     2401 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2581 accaactctt tttccgaagg taactggctt cagcagagcg ca
//
