LOCUS       sequence-369386-        6534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Unique Projection Barcode (UPB6)-Duodenum for Projection-seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Zhao Q, Yu CD, Wang R, Xu QJ, Dai Pra R, Zhang L, Chang RB
  TITLE     A multidimensional coding architecture of the vagal interoceptive 
            system.
  JOURNAL   Nature. 2022 Mar;603(7903):878-884. doi: 10.1038/s41586-022-04515-5.
            Epub 2022 Mar 16.
  PUBMED    35296859
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04515-5"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7903"; pages: "878-884"
COMMENT     Sequence Label: UPB-duodenum (UPB6)
FEATURES             Location/Qualifiers
     source          1..6534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR
     primer_bind     998..1020
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1082..1101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1140..1149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2601..3189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2654..2674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3072..3083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3610..3731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3621..3640)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3675..3694
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3893..4022
                     /label=AAV2 ITR
     rep_origin      complement(4259..4847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4339..4358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5018..5809,5810..5878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5641..5660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5879..5983)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6009..6464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6358..6377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      541 tgggggcggg gggggggggg gggcgcgcgc crgggggggg gggggggggs grggggsggg
      601 gsggggsgrg gsggrgrggk gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
      961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
     1141 ccaccatggt gagtaagggc gaggaagtga tcaaagagtt catgcggttt aaggtgagaa
     1201 tggaaggaag catgaacggc cacgagttcg aaattgaggg agaaggagag ggacggccct
     1261 acgagggcac ccagacagcc aagctgaaag tgacaaaggg cgggcctctg ccattcgctt
     1321 gggacatcct gagcccacag tttatgtacg gctccaaggc ctatgtgaaa catccagctg
     1381 acattcccga ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa agagtgatga
     1441 acttcgagga cggaggcctg gtgactgtga cccaggacag ctccctgcag gatgggaccc
     1501 tgatctacaa ggtgaaaatg agagggacaa attttccccc tgatggacct gtgatgcaga
     1561 agaaaactat gggatgggag gcctccaccg aaaggctgta tccacgcgac ggggtgctga
     1621 aaggagaaat ccaccaggct ctgaagctga aagatggggg acattacctg gtggagttca
     1681 agacaatcta catggccaag aaacctgtgc agctgccagg ctactattac gtggacacaa
     1741 aactggatat cacttcacac aacgaggact acactattgt ggagcagtat gaacggagcg
     1801 aggggagaca ccatctgttc ctgggccatg ggactggaag taccggctca gggtctagtg
     1861 gaaccgcctc aagcgaggat aacaatatgg ctgtgatcaa agagttcatg aggtttaagg
     1921 tgcgcatgga gggcagcatg aatgggcacg aatttgagat tgaaggagag ggcgaaggga
     1981 ggccttacga gggcacacag actgccaagc tgaaagtgac caagggagga ccactgcctt
     2041 tcgcttggga tatcctgtct cctcagttta tgtacggaag taaggcctat gtcaagcatc
     2101 ccgctgacat tcctgattac aagaaactgt ctttcccaga gggctttaag tgggagagag
     2161 tgatgaattt tgaagatgga ggcctggtga ccgtgacaca ggactcctct ctgcaggatg
     2221 gcactctgat ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat gggcccgtga
     2281 tgcagaagaa aacaatgggg tgggaggcca gcactgaacg gctgtatcct agagacggag
     2341 tgctgaaggg cgaaatccac caggccctga agctgaaaga cggcggccac tacctggtgg
     2401 agttcaaaac catctacatg gccaagaaac cagtgcagct gcccggctat tactatgtgg
     2461 acaccaagct ggatatcaca tcccacaatg aagactacac cattgtggaa cagtatgaga
     2521 ggtctgaagg acgccaccat ctgtttctgt acggcatgga tgagctgtat aagtaagaat
     2581 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2761 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2821 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2881 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2941 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3001 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3061 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3121 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3181 cctccccgca tcgataccgt cgacaatggc agctcgggca atcagtccgt gcgcctggtc
     3241 acgtcatcat cccacaatcg ctatgagacg gtggaaatgg tcttcattgc cacagtgaca
     3301 ggctccctga gcctggtgac tgtcgtgggc aacatcctgg tgatgctgtc catcaaggtc
     3361 aacaggcagc tgcagacagt caacaactac ttcctcttca gcctggcgtg tgctgatctc
     3421 atcataggcg ccttctccat gaacctctac accgtgtaca tcatcaaggg ctactggccc
     3481 ctgggcgccg tggtctgcga cctgtggctg gccctggact gcgtggtgag caacgcctcc
     3541 gtcatgaacc ttctcatcat cagctttgac cgctacttct gcgtcagcgg ccgcttcgag
     3601 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3661 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3721 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3781 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     3841 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     3901 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3961 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6001 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6061 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     6481 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gcca
//
