LOCUS       sequence-369392-        9597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  broad-host range plasmid to constitutively express fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE163
FEATURES             Location/Qualifiers
     source          1..9597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1794
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1795..2457
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3151..3173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3165..3182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3166..3182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3192..3211
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3192..3210
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3253..3339
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3436..3522
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3619..3705
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3802..3888
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3990..5189)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             join(6322..6324,6325..6327,6328..7038)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6367..6388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6628..6647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6975..6996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7059..7153
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7197..7856
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7263..7282)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      7921..8007
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8346..9140)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8457..8476)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9067..9086
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaagggcgaa gcccgcccgc
     1381 ctgccccccg agcctcacgg cggcgagtgc gggggttcca agggggcagc gccaccttgg
     1441 gcaaggccga aggccgcgca gtcgatcaac aagccccgga ggggccactt tttgccggag
     1501 ggggagccgc gccgaaggcg tgggggaacc ccgcaggggt gcccttcttt gggcaccaaa
     1561 gaactagata tagggcgaaa tgcgaaagac ttaaaaatca acaacttaaa aaaggggggt
     1621 acgcaacagc tcattgcggc accccccgca atagctcatt gcgtaggtta aagaaaatct
     1681 gtaattgact gccactttta cgcaacgcat aattgttgtc gcgctgccga aaagttgcag
     1741 ctgattgcgc atggtgccgc aaccgtgcgg cacccctacc gcatggagat aagcatggcc
     1801 acgcagtcca gagaaatcgg cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg
     1861 gaacgcaaag cgcatgaggc gtgggccggg cttattgcga ggaaacccac ggcggcaatg
     1921 ctgctgcatc acctcgtggc gcagatgggc caccagaacg ccgtggtggt cagccagaag
     1981 acactttcca agctcatcgg acgttctttg cggacggtcc aatacgcagt caaggacttg
     2041 gtggccgagc gctggatctc cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac
     2101 gtggtcaatg accgcgtggc gtggggccag ccccgcgacc agttgcgcct gtcggtgttc
     2161 agtgccgccg tggtggttga tcacgacgac caggacgaat cgctgttggg gcatggcgac
     2221 ctgcgccgca tcccgaccct gtatccgggc gagcagcaac taccgaccgg ccccggcgag
     2281 gagccgccca gccagcccgg cattccgggc atggaaccag acctgccagc cttgaccgaa
     2341 acggaggaat gggaacggcg cgggcagcag cgcctgccga tgcccgatga gccgtgtttt
     2401 ctggacgatg gcgagccgtt ggagccgccg acacgggtca cgctgccgcg ccggtagcac
     2461 ttgggttgcg cagcaacccg taagtgcgct gttccagact atcggctgta gccgcctcgc
     2521 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     2581 gacctgaatg gaagccggcg gcacctcgct aacggattca ccgtttttat caggctctgg
     2641 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
     2701 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     2761 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     2821 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac
     2881 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     2941 cgcagtacgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3001 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     3061 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3181 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctggggccg caattcccaa
     3241 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3301 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3361 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
     3421 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3481 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3541 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3601 aattaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3661 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     3721 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     3781 aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3841 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3901 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3961 gccaggaatt ggggatcgga ttcgagctct cagcgatcgg ctcgttgccc tgcgccgctc
     4021 caaagcccgc gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat
     4081 gcccacccgt tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg
     4141 acgatcgagg tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc
     4201 tcatccacct gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca
     4261 agcaggacca tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag
     4321 cctgtgccgt cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct
     4381 acagggccgg tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa
     4441 atgccgatcg tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg
     4501 gccggcacct gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg
     4561 gtcatgcccc gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc
     4621 cggcgttcgc ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg
     4681 ttcaaggctg ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg
     4741 agcacaggtc ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc
     4801 gcgcgctcat cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc
     4861 ccggtgatgc cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgctgtcgcc
     4921 atgatggcgt agtcgacagt ggcgccggcc agcgagacga gcaagattgg ccgccgcccg
     4981 aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc
     5041 gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg
     5101 cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga
     5161 attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc
     5221 gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg
     5281 ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc
     5341 tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg
     5401 ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg
     5461 ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc
     5521 gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg
     5581 gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc
     5641 agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga
     5701 caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg
     5761 gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag
     5821 acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg
     5881 atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgcta
     5941 agaaaccatt attatcatga agctcgcacg ctgccgcaag cactcagggc gcaagggctg
     6001 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     6061 tgtcagctac tgggctatct ggacaatgga aaacgcaagc gcaaagagaa agcaggtagc
     6121 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     6181 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     6241 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     6301 atactagtgg aggaagaaaa aatggtgagc aagggcgagg agctgttcac cggggtggtg
     6361 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6421 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     6481 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcgtgca gtgcttcgcc
     6541 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6601 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6661 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6721 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6781 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     6841 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6901 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     6961 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     7021 atggacgagc tgtacaagta agtaattagc tgagcttgga ctcctgttga tagatccagt
     7081 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     7141 tattggtgag aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg
     7201 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     7261 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     7321 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     7381 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
     7441 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     7501 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     7561 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     7621 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     7681 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     7741 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     7801 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     7861 tttaaggcag ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat
     7921 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     7981 gtgaacgctc tcctgagtag gacaaatccg ccgctaggag cttgcggccg ccagtgtgat
     8041 ggatatctgc ataaaaactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     8101 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     8161 tatttgccca tgggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     8221 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     8281 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     8341 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     8401 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     8461 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     8521 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     8581 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
     8641 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     8701 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     8761 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     8821 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     8881 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     8941 agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     9001 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     9061 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     9121 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat
     9181 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     9241 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     9301 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     9361 gcgttttccc ttgtccagat agcccagtag ctgacattca tcccaggtgg cacttttcgg
     9421 ggaaatgtgc gcgcccgcgt tcctgctggc gctgggcctg tttctggcgc tggacttccc
     9481 gctgttccgt cagcagcttt tcgcccacgg ccttgatgat cgcggcggcc ttggcctgca
     9541 tatcccgatt caacggcccc agggcgtcca gaacgggctt caggcgctcc cgaaggt
//
