LOCUS       sequence-36941-e        5404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Huff J, Czyz A, Landick R, Niederweis M
  TITLE     Taking phage integration to the next level as a genetic tool for 
            mycobacteria.
  JOURNAL   Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6.
  PUBMED    20692326
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2010-11-15"; volume: "468(1-2)"; pages: "8-19. Epub 2010 Aug 
            6"
COMMENT     Sequence Label: pML1342
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1058..1075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1229..1817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1309..1328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3269..3302
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3723..3756
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac agcgggcacg
       61 cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg acgccagctc
      121 tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc cgccatgcct
      181 acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc gcagagaacc
      241 cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg acgcctgagg
      301 agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca tacatcctgg
      361 cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac atcgtggacg
      421 acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg
      481 tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg cctcacgtcg
      541 cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc cccgaggcat
      601 tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc aagtcgctga
      661 agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg
      721 gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc
      781 acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta
      841 tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca
      901 aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc
      961 gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg
     1021 agcattgccg ttcccggtac ccgataccgt cggcttcctc gctcactgac tcgctgcgct
     1081 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1141 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1981 ctgacagtta ccaatatttc tacaattatt ggattgccgt ggtgcagggt ctcccattct
     2041 cggaacccgg tgagccgtcc catgttcggg gtttccctgc cctcgtactc accccggaac
     2101 gcgacggcgt cggtccagta gcaggtctcg gagcagtacg tcctccactg gccgtcgacc
     2161 ttctcgacga actagaattg gcacatggcc cgtcacaagg agttcgatcc ggacgtcgca
     2221 ctggacaccg cgatgcgggt gttctggcgc agcggctacg cgcacacgtc gaccgaagta
     2281 ctagaaagga ggaataatga acaagggcgt gatgcggccg ggccacgtcc agctgcgcgt
     2341 gctggacatg tcgaaggccc tggagcacta cgtcgagctg ctgggcctga tcgagatgga
     2401 ccgcgacgac cagggccgcg tgtacctgaa ggcctggacc gaggtggaca agttctcgct
     2461 ggtgctgcgc gaggccgacg agccgggcat ggacttcatg ggcttcaagg tggtggacga
     2521 ggacgccctg cgccagctgg agcgcgacct gatggcctac ggctgtgccg tggagcagct
     2581 gccggccggc gagctgaact cgtgtggccg ccgcgtgcgc ttccaggccc cgtcgggcca
     2641 ccacttcgag ctgtacgccg acaaggagta caccggcaag tggggcctga acgacgtgaa
     2701 cccggaggcc tggccgcgcg acctgaaggg catggccgcc gtgcgcttcg accacgccct
     2761 gatgtacggc gacgagctgc cggccaccta cgacctgttc accaaggtgc tgggcttcta
     2821 cctggccgag caggtgctgg acgagaacgg cacccgcgtg gcccagttcc tgtcgctgtc
     2881 caccaaggcc cacgacgtgg ccttcatcca ccacccggag aagggccggc tgcaccacgt
     2941 gtcgttccac ctggagacct gggaggacct gctgcgcgcc gccgacctga tctcgatgac
     3001 cgacacctcc atcgacatcg gcccgacccg ccacggcctg acccacggca agaccatcta
     3061 cttcttcgac ccgtcgggca accgcaacga ggtgttctgc ggcggcgact acaactaccc
     3121 ggaccacaag ccggtgacct ggaccaccga ccagctgggc aaggccatct tctaccacga
     3181 ccgcatcctg aacgagcgct tcatgaccgt gctgacctga gcggccgctc aattgagccc
     3241 gcctaatggc gggctttttt ttgatatcat aacttcgtat aatgtatgct atacgaagtt
     3301 atggcgcgcc aagagtcgca caagagttgc agacccctgg aaagaaaaat ggccagaggg
     3361 cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt agctagtttg
     3421 gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt accccctctc
     3481 ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga agcctgagag
     3541 ttgcacagga gttgcaacgc gtaaaaaaaa aagccccgcg attgcggggc tttttttttt
     3601 atcgataagc ttatttaaat ttaattaagc atgctctaga gtttaaacac tagtcgagaa
     3661 aaaaaaaagc gccgcaactg cggcgctttt tttttttcag gtatctagct tagtcaatgc
     3721 atataacttc gtataatgta tgctatacga agttatcccg gggcgtcagg cgccgggggc
     3781 ggtgtccggc ggcccccaga ggaactgcgc cagttcctcc ggatcggtga agccggagag
     3841 atccagcggg gtctcctcga acacctcgaa gtcgtgcagg aaggtgaagg cgagcagttc
     3901 gcgggcgaag tcctcggtcc gcttccactg cgccccgtcg agcagcgcgg ccaggatctc
     3961 gcggtcgccc cggaaggcgt tgagatgcag ttgcaccagg ctgtagcggg agtctcccgc
     4021 atagacgtcg gtgaagtcga cgatcccggt gacctcggtc gcggccaggt ccacgaagat
     4081 gttggtcccg tgcaggtcgc cgtggacgaa ccggggttcg cggccggcca gcagcgtgtc
     4141 cacgtccggc agccagtcct ccaggcggtc cagcagccgg ggcgagaggt agccccaccc
     4201 gcggtggtcc tcgacggtcg ccgcgcggcg ttcccgcagc agttccggga agacctcgga
     4261 atggggggtg agcacggtgt tcccggtcag cggcaccctg tgcagccggc cgagcacccg
     4321 gccgagttcg cgggccaggg cgagcagcgc gttccggtcg gtcgtgccgt ccatcgcgga
     4381 ccgccaggtg gtgccggtca tccggctcat caccaggtag ggccacggcc aggctccggt
     4441 gccgggccgc agctcgccgc ggccgaggag gcggggcacc ggcaccgggg cgtccgccag
     4501 gaccgcgtac gcctccgact ccgacgcgag gctctccgga ccgcaccagt gctcgccgaa
     4561 cagcttgatc accgggtcgg gctcgccgac cagtacgggg ttggtgctct cgccgggcac
     4621 ccgcagcacc ggcggcaccg gcagcccgag ctcctccagg gctcggcggg ccagcggctc
     4681 ccagaattcc tggtcgttcc gcaggctcgc gtaggaatca tccgaatcaa tacggtcgag
     4741 aagtaacagg gattcttgtg tcacagcgga cctctattca cagggtacgg gccggcttaa
     4801 ttccgcacgg ccggtcgcga cacggcctgt ccgcaccgcg gatcaggcgt tgacgatgac
     4861 gggctggtcg gccacgtcgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4921 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4981 actttagatt gatttcgtta acattatggg ccggtcaaga taggttttta ccccctctcg
     5041 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     5101 tgcacaggag ttgcaacccg gtagccttgt tcacgacggc tagcagaaag gaggatttca
     5161 actatcttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     5221 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     5281 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     5341 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     5401 accc
//
