LOCUS       sequence-369417-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs3 from Salmonella enterica NCTC13175.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: SeAvs3
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      451..498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(718..1377)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1292..1311
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1378..1480)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1872..1889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2006..2551)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2086..2105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaatggct aaccgccgct aaggatattg attcagcagc aaaaatactg attgattact
       61 gtttatcgtt agcagcagaa aatgctatcg ataaatccca agaatgggtg gaattagcac
      121 agaaagctgg attgaacaaa gatgttgatc tgcttgaaat tcgtatcttt cagttacgag
      181 gtaccccagc caatacagac aatcccaata atgcacaacg gagaatactg gaaaaaaggc
      241 aaaaaaggct tgaagctttt ctcttattgg gctcccagtt aaacgaacaa ctcaaatctc
      301 agcttgaagc cttaccagca attgaggatg agccaacgga tgacgacgaa gacttttgat
      361 atgacttgct ttagcactgg agacggctca caagacggac cacataatag cctaacccaa
      421 gacttttcta ctagtcctaa tgcgcaataa ctagcataac cccttggggc ctctaaacgg
      481 gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt
      541 ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc
      601 cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat
      661 cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta
      721 cgccccgccc tgccactcat cgcaatactg ttgtaattca tttaacattc tgccgacatg
      781 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      841 ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac
      901 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
      961 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1021 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1081 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1141 gtctttcatc gccatacgga actctggatg agcattcatc aggcgggcaa gaatgtgaat
     1201 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1261 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     1321 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     1381 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1441 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
     1501 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
     1561 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact
     1621 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     1681 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
     1741 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
     1801 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
     1861 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     1921 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     1981 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     2041 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     2101 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     2161 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     2221 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     2341 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     2401 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     2461 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     2521 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     2581 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     2641 atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg
     2701 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     2761 ttaattgcgt tgcgcaacaa ttttttgcca taagacgctt tcctgaaact cttctcattc
     2821 tcagcaggaa agcgttctct tctcaatact ctctggttat agagtattaa aaaataagga
     2881 gttataatcc ttgtagccca actgacataa ggacgatgct caatgtctga cagcctgctt
     2941 gttcgcacca gtagagatgg cgatcagttt cattatcttt gggcggctcg ccgcgccctt
     3001 cgactactgg aacctcagtc aactcttgtt gccctgacca ttgaaggggc atcaacgacg
     3061 gaaatgggct ctcagccagt ggttgaggat ggggaggagc tgattgatat tgctgaatat
     3121 tacggcagta acgagctcgc aacagcaaca actgttcgtt atatgcagct aaagcattca
     3181 acaatgcact cagatactcc atttccccct agtgggttac aaaaaaccat cgaaggtttt
     3241 gcaacccgtt ataaggcact tatacaaaaa ataccggtag aaacgttacg cactaaactc
     3301 gagttctggt ttgtgacgaa ccgtccagtc agtagcagct tcagtgaagc gatcaatgat
     3361 gccgcgaacc aacacgttac acgccatcca catgatctgg cgaaacttga gaaatttacc
     3421 gggcttcaag gcgctgagtt atcgatattc tgccagcttt tacatataga aggtcagcag
     3481 gacgatttat ggagtcagcg gaatatcctg ctaagagaat cagcgggata tctccccgac
     3541 ctggatactg aagcccctct gaaattaaaa gagctggtta acagaaaagc gttaaccgaa
     3601 agcgccgcaa atccttccat taccagaatg gatgtgttgc gtgctttggg ggtggatgaa
     3661 acagatcttt ttcctgcgcc ctgtcgtatt gaaagaatag aaaattccgt ctcaagaact
     3721 caagaggcga cgctggttca acgtgttgtt gaagcattcg gcgcacctgt gatcatccat
     3781 gccgatgccg gtgtggggaa atcaattttc tctactcata tagaggagca tcttcccact
     3841 ggttctgtta gcatcttata tgactgtttc ggactgggtc agtaccgtaa cgcgtcttcc
     3901 taccgccacc accatcgtac agcattggtt cagatggcta atgaaatggc atctcgtggt
     3961 ctctgtcatc cattgatccc aaatgctggt actggcatat cccagtatat gcgtgcgttt
     4021 ctgcatcgcc tttctcagag catttcaata ctccgggcct ctgagccctt ggccgtattg
     4081 tgtattatta ttgatgctgc ggacaatgca cagatggcgg cggaagaaat cggtgaaacg
     4141 cgttctttta tcaaagattt aattagagaa aagcttcctg atggagtctg ccttgttgca
     4201 ctttgccgac cttatagacg ggaattactt gatccacctc ctgaagcact cacattatcc
     4261 ctacaaactt ttaatcgcga tgagacagcc gctcatcttc accaaaaatt tccagatgcc
     4321 agcgaaagtg atgttgacga gttccatcgt ctaagctctt gcaacccccg ggttcaggct
     4381 ctgtcattat cacaaaatct tccactgaac gacacattga gacttttggg gccaaatccc
     4441 aaaacggtag aagatactat tggtgaagtg ctggaaaaat ccattgctcg cttacgtgat
     4501 acagccggaa tatctgaacg tgctcaaatt gatacgattt gttccgcact ggcaatattg
     4561 cgtccattaa ttccattatc tgtgctatct gccatttccg gagtagctgg ttctgctatt
     4621 aaaagtttcg cacttgatct gggacgcccg ttaatcgtta gtggcgagac tattcagttc
     4681 tttgatgaac cggccgaaac atggtttcag aggcgcttta ggccatcggc cgctgatctg
     4741 catcagttta ttactaaact gagaccacta acaaaagata gttcctatgc agcatcagtt
     4801 ttacctgcat tgatgctgga aggaaaccag ctttctgaac tgatcgagct agcgatatcc
     4861 tcacaagctc tgcctgaaac cagcgcggtt gaacgcaggg acatagaact tcaaagatta
     4921 cagtttgcgt taaaagcagc cttacgcaca ggtcgatacc aggatgcggc taaactggca
     4981 ctgaaagctg gtggagaatg cgcgggtgac aacaggcaaa gagtcctgct gagggacaat
     5041 atcgatctgg cagcaaaatt tgtgggaagc aacggcgttc aggaactggt ttcccgtaac
     5101 gcatttccag atactggctg gcctggctcc agaaatgctt attatgccgc aatactttcc
     5161 gaatatcctg aactctcagg agaggcccgc agtcgccttc gactcaccat ggagtggtta
     5221 acaaactgga gtcaattacc agatgatgag cggagcaggc aaaatgttac cgatcaggac
     5281 agagcggtaa tgctcattgc ctgcctgaat attcatggcg cggaagcggc agcaagggag
     5341 ctcagaaggt ggcggcctcg aaaactatct tttgacgctg gaaaaattgt tgccatgcag
     5401 ttactggccc acgcccgtta tgatgaactt gatcagttgg ctattgcggc tggaaacgat
     5461 atcagcctgg ttatgggaat tgtactggaa gcaagaaaac ttcaccgtcc agtcgctgaa
     5521 caagcaatca gaagaacctg gcgcttgtta aaaagtcagc gagtcagcat taaagacaga
     5581 aaccacgcta ataaccagac aatagcagca atcactggca tggttgaaat ggcgcttatc
     5641 caatctgttt gtactgaatc agaaagcatc cagttgttgg atcgttattt accaaaggtt
     5701 cccccctatg ctctgacttc tgagtatagt aaagaaagag ttgcttacgt ccgggcatat
     5761 gctctgcagg caaacctgat gggctctcaa ttagcgctta gcgatttagc ctccacagag
     5821 gttaaaaaag aacttatggc tgaaaaacgc cacggcgaat ctgatgacct gcgtcaactg
     5881 aagcagtaca gcggagtatt aatcccttgg tataatttat gggccaaagt aattcttggt
     5941 aaaacaagga aagcagactt agaaagtgag ctaagtgata ctcaaaaaga atcgacggct
     6001 attaaaggtc attcttactc tgagcattca ttatcatcaa atgagatcgc aaatgtatgg
     6061 tttgatattc tgatcgaagc aggtaatgta tcaaaagacg atgtggaaaa catcatcaaa
     6121 tggagtcagc ataaagggaa tagagtattc acaccaacgc ttcaccgttt cagttctgta
     6181 tgtgcagaga tttcagggct tggagagctt tcatatcact tcgcagaact tgccttatct
     6241 ttatggaggg atgagcactc tgatgctcag atcaaagctg acggctatat agacctttcc
     6301 cgttcactca tttcacttga tgaaccagaa gctaaagaat actttaacca agcgattgaa
     6361 gttacaaata agttaggcga tgaaaattta agtcgatggg aagcgatact tgatcttgct
     6421 gaatatgttg ctggtaaaac gcaagtccct cctgaaattt cctataaact agcccgatgt
     6481 gcggaactaa ccagagaata tgttgatcgt gataaacatt ttgcatggag tgatactgtt
     6541 gagattttgg ctgagttatg tccatcttca gccctagcaa taataagtcg ttggcgtgac
     6601 cgtacatttg gcaatcatag aagcatactg gcatggacca ttgagcatct tgtaaagaaa
     6661 aataaaatta atgcactcga tgcacttcct ttaatcacat ttgagaatga ttggcataaa
     6721 tgcgacttgc ttgattcagt tttatcctcg tgtactgatg acaaagataa gatcatggca
     6781 ttcgaagtgg tttaccacta tacaaaattt aacgtacaaa atatccaaaa tcttaaaaag
     6841 ctggatgcta tttctacatc attaggtatt gaacacacag aactgaaaga aagaatttca
     6901 ggtctacaac atactgagac ggtttcaaaa aaatccagtc tctcatcgaa tgataatgag
     6961 caaggccatg accaggaatg ggagtccatt tttaaagatt gtgatttatc gtctattgat
     7021 ggtattagtg cagcatacga aaaatttcgt aatgttcctg aattctattc caaagaaacc
     7081 ttcatcaaga aagcaataag ccgagttaag acgggcaaag aatgtagttt cattactgcc
     7141 attggtgcta tatttcactg ggggctttat gattttaaat atattcttga atctataccc
     7201 gacgaatgga catctcgttt aagcattaaa accaccctgg caggtttaat aaaagaatat
     7261 tgccaacgct tctgtatgcg aatcagaaaa agtcgcgttt acgagatttt tcccttcagt
     7321 ctggccagca ggctttctgg tataagtgaa aaagagattt tcggtattac cctggaggcc
     7381 attgcagaat cgccagagcc cgcaaactct gaccgtttat ttagccttcc tggccttctt
     7441 gttagtaaac tggagagtaa tgaagcgtta gatgtattat cttatgcctt ggatttattc
     7501 gacgaggtgc taaaagatga ggatggtgac ggcccatgga acgagaaatt atctccgcca
     7561 actcatgtag aggattcact tgcaggctat atttgggcgc ggctgggttc tccggaggcg
     7621 gaaatgcgct ggcaggcagc acatgcggtt ctggcactat gtcgaatgag tcgtacatgc
     7681 gttatacaag gaattttcca gcacgcaata aatgctacca ctttaccttt ttgtgatcgc
     7741 aatctgccct tttataccct ccatgctcaa ttgtggttga tgatcgctgc tgcaagggtt
     7801 gcgctggatg atggaaaatc gctgattccc aatattggtt atttctacca ttatgccact
     7861 actgatcagc cacatgtatt aatccgtcat tttgctgcca gaactttact tgcactgcat
     7921 gatagcgacc tgatctctat cccagcacaa gaagagaata aactccgaaa tataaaccag
     7981 tctacgactc tccctgtgct tgataaggtt gaagatcata gaggtgaaga ttcatatact
     8041 tttggtatcg actttggccc ttactggcta aaacctctgg gacgttgttt cggtgtatct
     8101 caaaaacagt tagaacctga aatgcttcgc attattcgtg atgttcttgg ttttaaaggt
     8161 agccgcaact gggatgagga tgagcgtaat aaacgacgct attatcaaga cagagataat
     8221 catcacagtc atggttccta tccacgggtc gatgactacc atttttactt gtcataccat
     8281 gcaatgttta tgaccgctgg gcagttatta gcgacaaaac cattagttgg tagtgactac
     8341 gacgatgtcg aggatgtttt ccaggactgg ttaagaagac atgatatttc tcggaacgat
     8401 catcgctggc tcgccgatcg gagagatatt ccccccaaag agcgctccag ttggcttaat
     8461 agcagttctg acaataggga tgaatggcta gcgtcaatct ctgaaaatgt atttaacgaa
     8521 acactatgtc ccagccccgg actattaacg ctatggggac gttggtctga cgtttgttca
     8581 gatcgaaaag aatctattat tgtccattct gcgttagtat cgccggagcg atctttatcg
     8641 ctcctcagag cattacaaac aactaaaaat gtatatgact ataaaatccc tgatgctgga
     8701 gataatcttg aaatagatca cgcacactat cagctaaaag gatggattaa agatattgct
     8761 gaatactgtg gaattgatga gtttgatccc tgggcaggta atgtaaggtt tccaatccca
     8821 gaaccagcct catttatcat tgatgcgatg aaattaacta ctgataaaga tcatcgggta
     8881 tggtattcac cttctgatgt tgaaccggcg atgatttcca gtatctgggg ccatctatca
     8941 ggtaaaaatg atgaggaaaa atcacatggt tataggctat gtgcttcaat acacttcata
     9001 aaatcagcat tagaaacatt caacatggat ctcattttag aggttgatgt tgatcgctat
     9061 tcacggaaca gcagatatga acggaataat gaaaatgagc tcgacaatat cccttcaagc
     9121 actcgactct tcctcttccg acatgacgga accatccaca cgctatacgg caattataga
     9181 aatggggaaa aaactagttg atgagcttga gctaaatgac tctgttgata cattaagcag
     9241 atggatggct catcatatcg cagagctcat ttatgatgct gaacattgta cagacgacat
     9301 cgtccgtaca gctaaacaag cggagattag ggactctatc tggtcattct ggtctaacag
     9361 atacgaattg ccaattggta gcagaccatt tcaggagctc gaacctattc taagaacctt
     9421 aaaaggtctt gatcctgaaa atgagcaacc gagatttttt tcaccttacc gagatctaat
     9481 taatgtagaa aaagaaacca gtgaggtcc
//
