LOCUS       sequence-369418-        8868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs3 from Klebsiella variicola PF-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: KvAvs3
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(315..332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(449..994)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(529..548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      7762..7809
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8029..8688)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8603..8622
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8689..8791)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt
       61 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
      121 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
      181 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
      241 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
      301 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
      361 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
      421 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
      481 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
      541 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
      601 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
      661 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
      721 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
      781 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
      841 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
      901 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
      961 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa
     1021 atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
     1081 acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact
     1141 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     1201 acattaattg cgttgcgcag atgttcagat tattcaaata attacaatag tatgttctgg
     1261 cttgggtctc taaacgtgga tatggtgaaa taaaataatt tttaaggatc aagattacta
     1321 tgaatctagc agtaaggaac gtagacaaag tcagagtatc acgtgcggga cacacttttc
     1381 atgaaaggtg gactgcgcgg cgagctcttc agcttgtgtt cccgaaggat aatcttttcg
     1441 cgatagtgat cgaaggccta tcccctaacg aagatctaaa gcttggaaaa gaggcagagg
     1501 atatagccga ccttgttctt tattatggcg atggagatac tttcgaaacc tgcacagccc
     1561 agcagatttt gcagttcaaa tacaaggaag cttccgagcc cgtcacatca tcctatctga
     1621 aaaaaacgat caagaaattc gccgcaacac tgcgaaattt taaggccagc atttcggatg
     1681 aagacatagt gaagaaactg tcattcgggt ttgtgacgaa tgcggaattt tctggtgagc
     1741 tttgggatgc tatcgcgtgc ttgaaatcgg gccgcgcacc ggggtccaat agcgccaaga
     1801 agcaacttga gtacctcaaa acctggtgca aggaagagaa ggtcgaagcg gaggatattt
     1861 tcctgctcat cgagtttcgc gctgcaacaa atgatttgcc tgcgcagaac cgattcttgc
     1921 gacgaacaat cagtgattgg tcggctgact caagcggact ggctgcaagg aggctgtttg
     1981 cactcgtaga gcttgtccga gagaagtcgc aaatcgaggg gcagggcaag aattctatca
     2041 ggcgtgaaga tatcctcgat gctcttgaat gtgatgagga tcagttattt ccagcggata
     2101 cgcggtttgt tgatgttggt gatgtcgttg agcgcagcgc ctcgcaagat gtcaaagacg
     2161 agatcgaagc cagcaatctt cccgtattcc tccatgctga cggcggtgtt ggaaaaactg
     2221 tcttcatcca gagcctgacc acatatctga aagatacttt cgaaatcgtg gtgttcgact
     2281 gtttcggagg cgggtcgtat cgctccgaag cccaagcacg tcaccttcct cgggttggcc
     2341 ttctgcaaat cgtcaatgaa ctggcgaccc gcggactctg cgatccactt tttccaactg
     2401 acagcgatca atatgggttg atcgaaattg cccgcaagcg tttcaagcag gcgagcgaga
     2461 ctgtcaaaag tcagtcttca ctgcaaggta tactggttgt tttggacgca gcagataatg
     2521 cgcagttgga agccgaggcg cgaaatgaaa tcgccttccc taggcttctc cttgcatcgc
     2581 tgagcgcaga acctatcgaa ggtgtgaagc ttctgcttac tgcgcgtcct cacaggatgg
     2641 aaagtgtaat cggaaagagc cagacgaagc gtctcgcgct cgaaccgttc acgaaggagg
     2701 aaacttggag ctttcttgaa acaaggcgta aaaatatcac cgatgtagaa ttttccacag
     2761 ttttcgcgcg ttcgcaagga aatgcccgcg tccttgagta cctcgtcgaa agttgggact
     2821 cgcatgtgtc cggcaatgcg ccgcagagcg aaatatccgt cgaagaattg atcgcacaaa
     2881 aatgcgtgaa gatattccgg gatctacata tggcaggctg gcttgagatc gagattcgcg
     2941 aattcttcgc cgctttgtcg cttcttccac cgcccattcc gttaactgaa cttgctgagg
     3001 ctctgggctg gtcagaaacg caagtcaaaa gcgcggcatc cgatcttgcg ccgatgcttg
     3061 agctggtcaa acatggtgcg atttttcgcg acgaacccac tgaaacctat atcaaggacc
     3121 actatgcaag agaggctgca gcccagcagt ccattgcaca aaggctccta acgcgccaga
     3181 aggattcaat gtacgctgca gaagcgcttc cgcatttcct tgtggtaatt ggcgacagcg
     3241 acagagctta tcagcttgct ggttccgatg agtttccttc tatgaacgag actgaatatg
     3301 gacggcgaaa attgaaactt gcgcgtcttt atgcggcatt ctcgcttgcc atacgagacg
     3361 acaacctaga cagagtactg agcttgacca tgcaactctc acaggtagcc tcggcaaatg
     3421 cgaggggtga tcagtttatc cggcgttctc cggcactggc gacgatcctt ggagattctg
     3481 atgcctctcg ccgcctgttc aatgatcgat ctggctggcg cggtgggcgc gatgcacgtc
     3541 tgatcgtcgc ttacagtttc accgatgaac tggacgaagc gcgcattcat caaaacaggg
     3601 ccattgggtg gataaattgg catcttaata atgatgatga gaccaaacgg tttgaccgct
     3661 cggggccgga ggcatctgac gtcgcggcaa ttatgttctt gagtgtactg aagaacgagt
     3721 actcgtcgtt taaccggaat atccagatat ggagtttcaa gtttgcactt tccgtaattg
     3781 aagaactgat cactctttgc gcacaacacg aagtctcgaa tggaagtgag gctttgcaga
     3841 ccttggccaa attcgcagca tcgaaaaggt gcctgtctct taccttgcaa atcggtctcc
     3901 tgtctaagga gtgtggcctt tcgaaaagcc aactcaaggc tgtttcgcta gcgacgagtt
     3961 tgctttcaca gagttacaag aacaaagttc cggaagataa ctccgactat aaaatggagc
     4021 tgcagggggc tattgctggt gcggcgatgg actccttaat cgtgaattca aggcaggccg
     4081 cgaagtggtt gctcaagtta tgtcgacatc gtaggccatc atgctacgac tatggcgaac
     4141 gtcatggcat taacggggtt tggatgtctg ttcaatctgc ttgcatcacg tcctggtcgt
     4201 caggcaaaga gctgtctttt catcatcttt tgccggagga agtgaaaaca gggcgaaagg
     4261 caaaatctat agccaccgaa gctgacttca gcgcgttcct tgatagcctc atcatgacaa
     4321 gagactacaa cagggggcga aagggtcgga agccagagaa gcgcaagcag ttttccgggc
     4381 atgaacgaga agatattgtg aagggcgtag cctgcgtcct gcagctagtc aaacctattg
     4441 aggcagccct tctgtctaag gttacgcttt caaatgacgt tctggccgaa ttcttgactg
     4501 tctggaaatc ttcgttgaga cccgatgtcc actggagagc tgaaaccgga agagacaatg
     4561 ttgcgcgaca cgtcggcatt gggcttgcca gactgctgct tcgccattgc gaaacggtcg
     4621 aaaagaaaga ggctgaagag ctcatagaaa tcattggctt gaaccggttt tcgctgggtg
     4681 acaagctaag cgtactcgct cttatcgcta ggtgccctaa tctgtccgat gtcgcaggag
     4741 cgtactccgc gatgatttcc agcgacatag taaaggatga ctacatcgaa caacgcggcg
     4801 agtcctacca ggatttggct gcatcgttgc taccgatgag cattggtgaa gcacaagagt
     4861 actacgcaca aggtttggtt caacttgatc agatgggtgg taatgacttc gacttgattt
     4921 actccgcctt gcactacgcc gcagaacagc ctggagggtt tgtgaagccg gaggtctcgc
     4981 accggctcat gaacctttgt cagacgatct tccagcatga gccatcaaag ttcggatgga
     5041 cactgttcgg acgtgccgcc gcttcatcca tcggctttcc ggcgatatac aagttgatac
     5101 gctgggatga tcaggatgtt gtagattatt cctacggact tccacagctt gcctgctacc
     5161 tcgcgaaagc gggacatctt gatgcgcgcc gtgcggcggt gcttcttaca ataagtgaag
     5221 atcatggctg gcatgagtgg caagtcggta agggtttgca cgatctgctt tcaattgcca
     5281 atcctaagga aaaggctgca atcttctctc ttgtggcagc gaagctcgat caagagcaca
     5341 gttttggtgg gtgggaaggg ctatgggaga gcctgctggg ctgtgtggat gccgttgaag
     5401 agatcaatga cgagagtttg cgtgatcatc tccaaggtca gtgggaagct gcgcggcgta
     5461 ggcgcgttat cgaaaatacc aaaagaaatt atggtggcgc gagcgctgaa tacggagtac
     5521 aggagcgtag caagaagcag gatgaacagg ctcgaaacaa agccgtcgcg gcgattgttg
     5581 ccaaatgtga ctttacatct gcatcatcgg tggatgcggc aattcaagaa gttgaggcca
     5641 gtgatgggct gctgtttggt agcagaagtc gcctgctaga ggaactccgg actgtttgcc
     5701 cgtatgacaa aagggtcaag tttcttgagg cactttgtga gtctaccgag cttgaatttg
     5761 atcgcgcact cgaccttata atcgaatgcg tggaggtttg gggtaatagt tcggtacatg
     5821 caacgaagag cgcacctgat ctgatcaaga agctttttgc attcaaggga tccgagcttt
     5881 tcgagctcca ctattcgggc atctccagac acatccttcg tctatccaaa atctgcggcg
     5941 atccaaaatt cgtgttgcaa actgttttag aaacggtagc gaaggagcgc cttgagctcg
     6001 gaggagacga atggcttcaa ctggcgacca gtctatctcg ccacactgac ccttcgacgg
     6061 ctttagatgc ttttgagaac ttgctttcca gttcagttgc aaaggttggt gatgaaatcg
     6121 gcgaaggtga gtatcgcgct gcgttcgcag gcaagagtga cgaaagtgac gtcctcgctg
     6181 atatcatctg gcatctgctt ggtgatagtg atgcctttgt cagatggagt gccgcacgaa
     6241 gtctgaaggg catgcttgat atcggattga cagaagatat agggtgcttg cttgatcgtt
     6301 ttgattcggt tgacaactca tctctggcct cagataagca tcattttgct tttctgaatg
     6361 cacagcaatg gctgttgatg ggattggcgc gcgcggctct gcataatgga gaaaagctca
     6421 aacctctgag ttcaaaaatt gcagcgctag caaagcgaac cgatttgcac gtgctcaaca
     6481 aacttcatct tgcccgatgc cttagacata tcgagggcga taaattcatg agttccgaac
     6541 tggagaaact gtgggtagat gttcaaacgc ctctaaaagg tatcgttgaa cgcgatggct
     6601 tgccggtcaa taaagaccga cgaactgatt ttgattttgg atatgaattt gataaaaccg
     6661 atattccaag cttggcaaga ctttttgata tatcgaaaaa tgaagccagt gattacataa
     6721 ccgatgaagt gatcaaacgt tggccaagtg caaaaagcat ttccgatttt cccgaaacgg
     6781 ttcgctaccg tcccgacgaa cagttcgaga cctatgctga acacattcaa cggcatgctc
     6841 gcctttttgc agcaactaca ctcgtgaaga ccttgcccgt ggcacggcaa tgctattatt
     6901 tggatggcct gaacccttgg caagaattcc tacaagacga agatgtttcg tttgatgatg
     6961 gctcatggtt atcagatcat aaagactgtg ttccagaaga ggctcgggca tatctactcg
     7021 gcgagcgcaa agaaaatcaa gaaacgcttc ttggcaaaga gtcgctgttt gcaaaggtcg
     7081 gtttttccca agactcggat gatcagttcc taccgcttta cggttactgg acttcaccgg
     7141 atggcgtaca tgtccgtata gattccgcgc tgattaaggg gcgtggtgcg gtcgggcaat
     7201 gtacaaagtt ttcaaggtac cctgattacg atctttggtt gccacgtttt ggttcagatg
     7261 gacgtgttga tcgctacact gcgaataatc cgttcaaccc attgatttgg gaaccggaga
     7321 aatatcccat tggtattgac gagggtgaag agtgggccgc tcggggagct aaagcccgtc
     7381 caaaactagg gctgtccctc aataagttgc ttggactggc ctccgatgat gatgaacggc
     7441 tgtggcatga tcatacaaat agactggtat tgaagagcgg agtctggggt gagtggaggc
     7501 aagaaccaaa tgctcgtagt aaatatcggg atgaggggac tatcctgtgg gcagaacgtg
     7561 actggctcga cgaagtgctc aaatccagca aacgaggcct catcttcacg ctcagtttct
     7621 caaaatacaa atcaagcgac tcatctggca tccgagaacg gtatgtaatc ttgaagcgag
     7681 gtggtgtttc cccccgcatt tggtatgcca agaaggcatc taaaagggcg taatagttga
     7741 gtaatacact cttcgcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     7801 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     7861 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     7921 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     7981 aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     8041 ctgccactca tcgcaatact gttgtaattc atttaacatt ctgccgacat ggaagccatc
     8101 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     8161 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     8221 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     8281 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     8341 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     8401 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     8461 cgccatacgg aactctggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     8521 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     8581 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     8641 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     8701 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     8761 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     8821 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaa
//
