LOCUS       sequence-369419-        8665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs3 from Arcobacter cryaerophilus M830A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: AcAvs3
FEATURES             Location/Qualifiers
     source          1..8665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             156..167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6458..6505
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6725..7384)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7299..7318
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7385..7487)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7879..7896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8013..8558)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8093..8112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
       61 atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgcatt
      121 tacactttat gcttccggct cgtatgttgt gtgaaaaaga ggagaaacca tatgaaaata
      181 tttgtatcat atagtaggaa ggacaccatc tataaagacc gtttgatctc cagcatccta
      241 aaactcaagg atgacggcat cagcagcatc tggatcgatg aacgtaagat taagccgggt
      301 gatgagttcg acgacgagat tcaagaggcc atcaacacgt ccgatatttt tctcctgctg
      361 ctgtccgaga acttttggac cagcccgtat atccaacgtt acgagcttcc ggcgatccaa
      421 aaaaagtata aaaaagataa ggttaaaatc atcccaatcg ttctgaagag caccaaggac
      481 ctgtttgaat atgaaggtat taacaaaaaa ctggctatgc cgcaaggtaa agcagttagc
      541 gatatccgtc cgcagtcgaa gatttttaat gagatctact tccacttgaa gaatctgatt
      601 aattcccaaa acatcggtct gaattatcat gttttggatc tatgcaaaga gagcctgttc
      661 tctctcaacc catacgacga ctacatcaaa ctgaaatacg aaaaaattga atacatcaac
      721 aataacgagt accgcatcat tttcaccaag tactacgaga atagcaccaa gaagaccttc
      781 agcaaacaca ttatcctcaa cgataaaatt aaatctctgt actccgagct gtacgacagc
      841 ctgtacttca agcctaagtc gaagtatatc aagatcaaag agaaactgct ggagcagaag
      901 gagctgtcaa agaacgaaat cctgcacatc ctggagatca ccatagaccg cctgtttccg
      961 gttagctcga agatcccaaa agtggatagc tacatccaga ccattgatat caagaatctc
     1021 aaagatatca tcattcaaaa cgagttttcc aagattcacg tttacggtga tgctggtctg
     1081 ggcaaaacct ctacgttaag ctctctcaaa accatcttcc cggattatct gactattatc
     1141 tacgatagct ttggcgcagg tacatataag aacatcggtg aaaagcgcca cctgaagaaa
     1201 tccatcttga cgcagctcat caatgagatt gcggttaaca ccggtctgtc gctgcacatt
     1261 gacaaggaaa tcgacctgaa cgatctggaa tttgagttca aacagcagct gcagaatgcc
     1321 agccgcctgc ttaacgaaaa aaagatcttt attataatcg acgcagccga taataatgtc
     1381 gaggctgcga actactttca agaggatccg tttatcaaag aattatggaa catccaactg
     1441 ccgaacaacg tgtatctgat tatgagctcg cgtgacggtc tgcgtaagga gtccctgtgt
     1501 gcaagccgcg acgtgaagga cctgaagctg tatggctttg atgagatcag ttcgggtctg
     1561 ttcctggcca agagctaccc gaatattgac gaggagatta gcaaaaaatt ctatgaccgc
     1621 accaagggta atccgaggct tcaaaattac atcatcgaag aatgcaacgg cgacatcgtg
     1681 aaattagaaa acatcctcaa ccgtaaagat gagatctccc tggaacatat attcgaagat
     1741 atttggaata gcgctctgtc cgtgatccaa ccgattacga aaaaccatct ggaggaatta
     1801 atttgtctgt cccggcctgc gaaattggac gatttcatcc tggcgagcgg cattaccaaa
     1861 gtcgacgctc tgaaggtgat tgatacgctg tcaggagtta tcctgtcaga aaacaactct
     1921 atcggctttc tggatgagga cttcgagaac ttcttggtca gcaagttgac cgaattggag
     1981 aatcttgaga cccataagcg catttctatg aacctgttga ataatgttaa taaaaacgag
     2041 ttcgccgctc gttttatcag ctttcatttg gaaaaagcga aagaaagcga attgttgatc
     2101 agcctggcac tgaactccga tctgtccgtt attaaagacc aaattgagcg tgaggtgatt
     2161 aagaaggacc gtatttcgac tgcgctgcgg attagctacg aaaagaagga ctacaaaaac
     2221 ctgagcaaac tgctgtatat atctgccgaa ctgctggaga agaatgagtt gctaaaccat
     2281 attctgcaag acgaatatct ggacctgcta tctatctaca ccaatcaaga gaatgctatt
     2341 gagttattga ttaagtacag gaagaacctg aaatccttca actaccactg cgcgtatctg
     2401 ctgagtaaga agaacccgga aaaagcaaaa gaatacttgt ctttcggtga tatttggttg
     2461 aaaaaatacc tcgaagacga ggatcgtaaa cacaacgaat tgaacgcgat tgacatcgcg
     2521 aagaagattt gtaccttgta ccatctgaag gacaagcgtc gtctgttgaa aaccctcaag
     2581 aattggaagc tgtggttctt cttcaaagtg atcaacgagc tgccgctgga attcttaaaa
     2641 gaaattacct tggaagtgca gaacgaatta tatccgctgc tgaagaaacg tctgcatccg
     2701 ttcgttcgtg cattactggt agtaaaactc caaaaagcgg gtcataaagt tgaaacgaat
     2761 atagttaagg atgttgcgaa aagctttaat ttattcatcg ataaataccc ggacagtcac
     2821 aaaaagttat ccaacaacaa ttataacacc gagcgcagcg acatcaataa aatttcgatt
     2881 gacttctcag tcatcgcgct gaagaataaa gtaaacaaaa aattcgtgct gaagttctta
     2941 tccctcagca gcccgagcaa ttttaacaac ctgtacgaca cacatagctt acgctacttc
     3001 gacgacacct ttctttgcat ttccttggac agtgccttga aaaacaagaa gtataatttc
     3061 gacaaagtgc tgaacagcat tccaaagaat ctgaagaagc agtataagaa agactacgaa
     3121 aaaaagaaga agctggaatt aattaacatc ttatatccgt attatgaact actgggtaaa
     3181 gtcatcgtca agaaacttga attcaaccag atcaaggagg agtgggaaaa cctcaaacat
     3241 cgtaatattc cgtatcagta ctatcgcagc gatgcgattc tgaagagcta ccaagcattg
     3301 gtactggtga aaattctgat cattagcaac gccaagatta acgatatccg tgagttcgtg
     3361 aagtacacta agagcattta catcaaaaac cacatttctg aggtgttctt acacaataac
     3421 ctgttctatc aggcatatga gattatcgac tacgcaatcg aagaagagtt aaaagagaaa
     3481 aagccggcgc gtgagaagat cgaccttttt ctgacgtatt cgaccctgat gaaagacttc
     3541 gataaagaga agtcccgtga atatttcgaa ctggcgctgg aagcttcgaa gggcattgac
     3601 gagaacatct ataacctgta tcgcatcatt ttcttcttca ccaacaacag tcttaacgtg
     3661 ctgaacgata acgagaagat agagctggcg aataagcaca tccagctgtc cgaaaacacg
     3721 ataccctacg tgagcgagac ggagaaactc gtgctgaacg actctttcaa aatcatcacc
     3781 aagctgaacc cggcgattgc gttggctacc agcattaagt ggcataacaa gatcatccac
     3841 aaaatcgagg ataccatcta catttacctg aaggaaatcc tacaagaaaa cttaatgagc
     3901 atcgaagatg tgctgagcat gcgttacctg attaaggagc cgaaaatcgt tgatatcttt
     3961 ctgattattc tgcgtaatgt gaagagcaat aaaatcttgt tcaacaagac cttggaaatt
     4021 tcttacgatt ttattcgtaa aaaccttaaa gacgacgaac agaacaactg cctcaaggag
     4081 ctgctgcagt ggctggaaaa agaaaactat cagagccacc gtatgaccaa caaaatcaag
     4141 ggcttctgca aattctacga aaagttggag agcaaaagct ctagccatat cggtacgacc
     4201 gattcgaaag agcgcgattg ggatgaattc ttcaacagct atgataacga cataattaag
     4261 gctctgccgg aatttatcca attgagttat cgtaatatcg agaatacctt tgaggagtgc
     4321 attaaccgta ttccgtacga gaaaagagaa gagataatca tcctgctgag caacccggat
     4381 gtttccaact attacattga cagctacggc gaagcgctgg cgctttgcct gctaaaatgg
     4441 aaaagcgacc cgcgtatcaa gaagctgagc aaggagataa gcgaggactt catcctgaat
     4501 cactttgcga ttttcctgga cttctatcag tatccgaagt gctttgataa cttttctaag
     4561 attttaagta aagaggagat ctccaagatt atagtttgtc agagcgcgaa taacatcaac
     4621 aaactgggct atctgaacaa ctacgaaaag ttgtttaatt acattaagta cctgatcggc
     4681 gaagaagatt ccaagaacgt tttgtacgat ctgatcctgc gttatgagaa acgccttgag
     4741 atcgagaaca tcgacgagtt cgagaacaaa gaaaacattt atagctatct gtataaactg
     4801 ctgggccatt ccgacaaacg aattcgttgg aaaaccctgt atacctgtaa agagatgatc
     4861 attaacaacc ccgatatcat cacggaattt ctaaaccagt ttgacaacta caagggtttc
     4921 ccgtttttcg atgatatgag cttttatcca attgcgagca aagaaagcat gatgattttg
     4981 ttctttcgtc tatcattcta cattccgaac agcctgaagt cttacatcaa gaagtttatc
     5041 gacatatcac tagacgagga attcccgcat attattatac gtcacatcgc caagcgtatc
     5101 atcgaaaata tagaaaaagt gtttccgaat accgtttcta acgaaatctt ccagaaagtc
     5161 aaattcttga atggtccgcg tagctgttat ctcaacgaag atcgccggta ctctgttggt
     5221 tatcgtgaaa gggagttcaa tcgttttgac tttgacagca ttgacactat cccgtactgg
     5281 tatcaaagca tctgcagcgt gttcaaggtt gatatggcag atatcctgga aaaagcggaa
     5341 acctggatca tcgacaaatg gaatatcaac actcgccgtg atctggagtg catcaatatt
     5401 tggggtcgtg atgacaccta ttccttgaca aatcaccgtc acggctctct tccgatcgtg
     5461 gaagaaccga ttcgccacat cgagtaccac gtgatgtttc tggtggtggg tgagtttctc
     5521 aaggaaatcc cggtgaacgt cagccactat tcggatggcg atttctgcga atatcaggag
     5581 ttcatcgacc gctactttaa cgactacgag aactggtttt acgaggaacg tggtattaag
     5641 ccgatggaag actttctctt cggcaagttc accaaaaagt acgacgagaa ccgttttgac
     5701 gagctccttg gcgttaataa agaaaacttt attctgtgca gcaactacga cgttaacagc
     5761 ctggactatt cggagaccat cgtgataaac agcgcgctgg tcagttctct cactgcgaaa
     5821 tcattgcaaa atgccttgca aaacacggat tcccaccatt ttaagttacc atttctgaac
     5881 gaaaacggtt ttgaaatcaa ccatggtaaa tatcagctgc gcggttggct gcgcgaggtt
     5941 tcccaggaga gacgtgagaa taacaaagac cagtatagcc acaaacagag catcaatatc
     6001 gacatctcct cggaagagat tatgaaaaac aagacctctg agaaagacat cattattgaa
     6061 aactgggcag atgaggaaat cgaagagtac gactaccaca ttaccagcag cggttaccgt
     6121 attaagatta acaaggaatt tatcaagaag tatcttcagt ctttgaacat ggatatgatt
     6181 atcgagattc agataaacat ctccgaacgt gaaaaaaatt catcttacaa agacaaatac
     6241 tccaagaaga gccaaattta catcctgcgt cagaatggcg aaatcgaaaa atacgaggag
     6301 aatgttattg tcgcgaaagg cattagcctg gttaagaaaa agttggatga ttatagctgc
     6361 aataccctga caaaatggaa actgcattat gaggtagagt gccgttacgg caaaaactat
     6421 aaatactaag taattagaaa taaattttac gcaataacta gcataacccc ttggggcctc
     6481 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
     6541 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     6601 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     6661 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     6721 aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt aacattctgc
     6781 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     6841 tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat
     6901 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     6961 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     7021 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     7081 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     7141 gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg cgggcaagaa
     7201 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     7261 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     7321 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     7381 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     7441 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     7501 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     7561 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     7621 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     7681 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
     7741 catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     7801 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     7861 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     7921 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     7981 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     8041 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     8101 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     8161 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     8221 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     8281 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     8341 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     8401 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     8461 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     8521 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
     8581 taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     8641 ccatacgata taagttgtaa ttctc
//
