LOCUS       sequence-36942-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4178 (=HL 862 + pHL 1360)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..84)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      237..323
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      415..442
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    535..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcatggatg agctctacaa ataaaagcta gcttggctgt tttggcggat gagagaagat
       61 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      121 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      181 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      241 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      301 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      361 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
      421 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
      481 cattcaaata tgtatccgct caggatccat aaatgtgagc ggataacatt gacattgtga
      541 gcggataaca agatactccc gggtaaggag gaaaaaaaat ggctaagggg caatctttac
      601 aagatccgtt cctgaacgca ctgcgtcggg aacgtgttcc agtttctatt tatttggtga
      661 atggtattaa gctgcaaggg caaatcgagt cttttgatca gttcgtgatc ctgttgaaaa
      721 acacggtcag ccagatggtt tacaagcacg cgatttctac tgttgtcccg tctcgcccgg
      781 tttctcatca cagtaacaac gccggtggcg gtaccagcag taactaccat catggtagca
      841 gcgcgcagaa tacttccgcg caacaggaca gcgaagaaac cgaataaggg cccatggtac
      901 gcgtgctaga ggcatc
//
