LOCUS       sequence-369424-       18078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of 3xFLAG-HUWE1 delta169-189.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18078)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG-HUWE1_delta169-189
FEATURES             Location/Qualifiers
     source          1..18078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(16505..16525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16923..16934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17001..17018)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17007..17214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(17262..17267,17268..17284,17285..17290)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17479..18012
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc cgtcgacatg aaagtagaca ggactaaact
     3361 gaagaagaca cctactgagg ctcctgcaga ctgcagagcc ttaatagaca aactcaaagt
     3421 ttgtaatgat gagcaacttc tcttggaact gcagcagatc aaaacatgga acattggaaa
     3481 gtgcgagtta tatcactggg tggacctgtt ggaccgcttc gatggaatac tggcagatgc
     3541 tggacagaca gtggagaata tgtcatggat gctcgtatgt gataggccag aaagagagca
     3601 actgaaaatg cttctcttgg ctgtgttgaa cttcacagcc ttgctcattg agtacagctt
     3661 ttcccggcat ctgtacagtt ccatagagca tttgacaact ttattggctt cctctgatat
     3721 gcaagtggtg ctggcagtcc tcaatctcct atatgtattt agcaaaagat caaactacat
     3781 cactcgtctg ggatctgaca agaggacccc gctgctaact cggctacaac atttggcaga
     3841 gaaatatcca cccagtgcaa ctacactaca ctttgaattc tatgcagatc ctggggccga
     3901 ggtcaaaatt gagaaaagga caactagtaa cacactacat tatattcaca tagagcaact
     3961 tgacaagatt tcagaaagcc cttctgaaat catggaatct cttaccaaaa tgtacagcat
     4021 tcctaaggat aagcagatgc tgttatttac acacatacga ctggcccatg gcttttctaa
     4081 tcacaggaag cgattgcagg cagttcaggc cagactgcat gcaatatcta tattagtgta
     4141 ttccaatgcc ttgcaggaat cagcaaacag tatcttgtat aatggcttga tagaggagtt
     4201 agtggacgtg ctgcaaataa cggataagca gcttatggag attaaagcag cttctttacg
     4261 aacattaaca tcaattgtcc acttggagag aactcccaaa ctcagcagta ttattgactg
     4321 tactggaact gcctcctacc atggattttt gccagtgctt gtaaggaact gtatccaggc
     4381 catgattgat ccttccatgg atccataccc tcaccagttt gccactgctc tcttctcttt
     4441 tttataccat ctggccagct acgatgctgg tggtgaagcc ttggtctcct gtggaatgat
     4501 ggaagcctta ttgaaggtca taaagtttct tggcgatgaa caggaccaga taacatttgt
     4561 caccagagcc gtcagagtgg ttgaccttat caccaacctg gatatggcag cttttcaatc
     4621 ccatagtgga ctttctatct tcatttatag acttgagcat gaagtagatc tgtgccgaaa
     4681 agaatgtccg tttgtgatca agccaaagat ccagagaccc aatactacac aagaaggaga
     4741 ggaaatggaa actgatatgg atggagtcca gtgtattcca caacgagcag cacttctgaa
     4801 atccatgttg aatttcctca agaaggccat ccaagaccct gctttctcag atggcatacg
     4861 acatgtgatg gatggttctc tgcctacctc cctgaaacac atcatcagca atgcagaata
     4921 ctatggccca tcactcttcc tcctagctac tgaagtggtg actgtgtttg tatttcaaga
     4981 accatcactg ctctcctcac tccaggacaa tggattgaca gatgtcatgc tgcatgcact
     5041 gcttatcaaa gatgttcctg ctacccgtga agtccttggc tccctcccaa atgtattcag
     5101 tgcactctgt ttgaatgccc gaggtcttca gtcttttgtt cagtgtcagc cttttgaacg
     5161 cctcttcaaa gttcttctgt ctccagatta cctcccagcc atgcggagga ggagaagttc
     5221 tgatcccctt ggggatactg catccaacct ggggagtgct gtcgatgagc tcatgagaca
     5281 tcagcccacc cttaaaacag atgcaacgac tgccatcatc aagttacttg aagaaatctg
     5341 taatcttgga agggacccca aatacatctg tcagaagcca tcgatccaga aggcagatgg
     5401 cactgccact gctcctcccc caaggtctaa tcatgccgca gaagaagcct ctagtgagga
     5461 tgaggaggaa gaggaagtac aggccatgca gagctttaat tctacccagc aaaatgaaac
     5521 tgagcctaat cagcaggttg ttggtacaga ggaacgtatt cctattcccc tcatggatta
     5581 catccttaat gtgatgaaat ttgtggaatc tattctgagc aacaatacaa cagatgacca
     5641 ctgccaggaa tttgtgaatc agaaaggact gttgcctttg gttaccattt tgggtcttcc
     5701 caatctgccc attgactttc ccacatctgc tgcctgtcag gctgttgcag gtgtctgcaa
     5761 atccatattg acactgtcac atgaacccaa agtccttcaa gagggtctcc ttcagttgga
     5821 ctccatcctc tcctccctgg agcccttaca ccgccccatt gaatcccctg ggggctcagt
     5881 gttgttgcga gaactggctt gcgcaggcaa tgttgctgat gctaccctct cagcccaggc
     5941 cacacctctg ctgcatgcac tcactgctgc ccatgcctac atcatgatgt ttgttcatac
     6001 ttgcagagtt ggacagagtg aaattcgttc catctccgta aaccagtggg gctctcaatt
     6061 gggtctgagt gttttgagca agctgagcca gttatactgt tccctggtgt gggaaagcac
     6121 tgtcctcctc tctctgtgta ccccaaacag cctaccatct gggtgtgaat ttggccaggc
     6181 agatatgcag aaactggttc caaaggatga gaaggcaggt acgacccagg gcggaaaaag
     6241 atcagatggg gaacaggatg gagcagctgg aagtatggat gcttctaccc agggcttatt
     6301 agaaggcatt gggctagatg gtgacacatt ggctcccatg gagacagatg aacctactgc
     6361 ttcagactct aagggcaaat ctaaaatcac accagcaatg gctgccagaa ttaagcaaat
     6421 caagcctttg ttatcagctt cctccagatt aggccgagca cttgctgagc tatttggact
     6481 tcttgttaaa ctttgtgtgg gatctcctgt ccgccagaga aggagccatc atgctgccag
     6541 caccactaca gcaccgacac ctgccgcgcg atcaacagcc tcagctctca ctaagctctt
     6601 gactaagggg ttatcttggc agcccccacc atatacacct actccccgat tcaggctgac
     6661 attcttcatc tgttcagttg gtttcacatc cccaatgctg tttgatgaga ggaagtatcc
     6721 ctaccacctc atgctgcaaa aatttctctg ctccggaggc cacaatgctc tttttgaaac
     6781 tttcaactgg gctctgtcca tgggaggtaa agttcctgtt tctgagggat tggaacactc
     6841 agacttgcct gatggcacag gagaattcct agatgcctgg cttatgctgg tggagaagat
     6901 ggtgaatccc accacggtgc ttgaatctcc acattcgctg cctgccaaat tgcctggagg
     6961 tgtccagaac tttccccagt tcagtgcact gcgcttcctt gtggtaactc agaaagcagc
     7021 ctttacttgc atcaaaaact tatggaaccg gaaacccctg aaggtatatg gtggacgaat
     7081 ggctgaatcg atgctggcca ttctatgcca catcctccga ggagaacctg tgattcgaga
     7141 gagactaagc aaggagaagg aggggtctcg aggagaagag gatacagggc aagaggaagg
     7201 tggctcccgc cgggaacctc aagtcaacca gcaacaactg caacagctca tggacatggg
     7261 cttcacaagg gaacatgcaa tggaggcact gttgaacacc agcaccatgg agcaggccac
     7321 agagtacctt ttaacccacc ctcctccaat catgggagga gttgttcggg atctcagcat
     7381 gtctgaagag gaccagatga tgagagcaat tgctatgtct ctgggacagg atattccaat
     7441 ggatcaaagg gcagagtcac ctgaggaagt tgcttgccgg aaggaggaag aggaacggaa
     7501 agctcgggaa aagcaggagg aggaagaggc taaatgtcta gagaagttcc aggatgctga
     7561 cccgttggaa caagatgagc tccacacttt cacagatact atgttgccag gctgcttcca
     7621 ccttcttgat gagctgccag acacagtata ccgtgtgtgt gacctgatca tgacagcaat
     7681 caaacgtaat ggagcagatt atcgtgacat gattctgaag caagtagtca atcaggtgtg
     7741 ggaagctgct gatgtattga tcaaagctgc tcttcccctg acaacaagtg acacaaaaac
     7801 cgtgtcagag tggataagtc agatggccac actgccccag gcctccaatt tggctactag
     7861 aatcttgctt ttaacgctac tttttgagga gttgaagcta ccttgtgctt gggtggttga
     7921 atcaagtggc atccttaatg tcctaatcaa actcttggaa gtggttcagc cctgcctcca
     7981 ggcagccaag gagcagaagg aagtccagac cccaaagtgg atcacaccag tgttgctcct
     8041 gattgatttc tatgaaaaga cagccatctc ctcaaaaagg agagcccaga tgactaagta
     8101 cctgcaatcc aacagcaaca actggcgctg gtttgatgat cgctctgggc gttggtgtag
     8161 ttacagtgca agcaacaata gcactattga ttctgcctgg aaatctggag agacaagcgt
     8221 gcgattcact gcaggccgaa gaagatacac ggtccaattc actacaatgg tgcaggttaa
     8281 tgaggaaaca gggaaccgac gccctgtgat gctgactctc ctcagggtac ctcggctgaa
     8341 taaaaattca aaaaacagca atggacagga actagagaag acgctggaag aaagcaaaga
     8401 aatggatatc aaacgtaaag aaaataaagg caatgatacc cctttggccc tagagagtac
     8461 aaacactgaa aaggagacaa gcctggagga aacaaaaatc ggggagatcc tgatccaggg
     8521 cttgacagaa gatatggtga ctgttttaat ccgggcctgc gtgagcatgc tgggagtccc
     8581 tgtggaccca gatactttgc atgccaccct tcgtctctgt ctgaggctca cccgggacca
     8641 caaatatgcc atgatgtttg cagaactgaa gagtacccgc atgatcttga atttgaccca
     8701 gagctcaggc ttcaatgggt ttactcccct ggtcaccctt ctcttaagac acatcattga
     8761 ggacccctgt acccttcgtc ataccatgga aaaggttgtt cgctcagcag ctacaagtgg
     8821 agctggtagc actacctctg gtgttgtgtc tggcagcctc ggctctcggg agatcaacta
     8881 catccttcgt gtccttgggc cagccgcatg ccgcaatcca gacatattca cagaagtggc
     8941 caactgctgt atccgcatcg cccttcctgc ccctcgaggc tcaggaactg cttcagatga
     9001 tgaatttgag aatcttagaa ttaaaggccc taatgctgta cagctggtga agaccacccc
     9061 tttgaagccc tcacctctgc ctgtcatccc tgatactatc aaggaagtga tctatgatat
     9121 gctgaatgct ctggctgcat accatgctcc agaggaagcc gataaatctg atcctaaacc
     9181 tggggttatg acccaagagg ttggccagct cctgcaagac atgggtgatg atgtatacca
     9241 gcagtaccgg tcacttacgc gtcagagcag tgactttgat acgcagtcag gtttttccat
     9301 taatagtcag gtctttgctg cagatggtgc ctccactgag acttccgcat ctgggacctc
     9361 ccaaggagag gcttcaactc cagaggagtc tcgagatggg aagaaagata aagaagggga
     9421 ccgggcctct gaggaaggca aacagaaagg caagggcagc aaacctttaa tgcctacctc
     9481 cactatcctt cgtcttctgg cagagttggt gaggtcctat gttggtattg ctaccctgat
     9541 tgccaactac agctacactg tgggccagtc tgaactgatc aaagaggact gcagtgtgct
     9601 agcttttgtt ctggaccacc tgctcccaca tacccagaat gcagaagaca aggacacccc
     9661 tgccttggcc cgcctgttcc tcgcaagcct ggctgctgca gggagtggca cagatgccca
     9721 ggtggcccta gtgaatgaag taaaagcagc ccttggacgg gcactggcta tggctgagag
     9781 tacagagaaa catgccaggc ttcaggcagt gatgtgtatc atcagtacta tcatggagtc
     9841 ctgcccctcc acctccagct tctacagcag tgccacagcg aagacccagc acaatggcat
     9901 gaacaacatc attcggcttt tcctgaagaa gggactggtt aatgacctgg ccagagtacc
     9961 tcacagctta gacctgtcca gtcccaacat ggccaacaca gtcaatgctg ctctgaagcc
    10021 tttggaaaca ctttcccgga ttgtgaacca gcccagtagc ctttttggca gcaagagtgc
    10081 ttctagcaag aacaagtctg agcaggatgc ccaaggagcc tctcaagatt ccagtagcaa
    10141 ccagcaggac ccaggcgagc ctggggaagc agaagtgcag gaggaggatc atgatgtcac
    10201 tcagacagag gtggcagatg gggatatcat ggatggggag gctgaaaccg actcagtggt
    10261 gattgctggg cagcctgagg tgctcagttc acaagagatg caggttgaga atgagctgga
    10321 ggacctgata gatgagttgc ttgagaggga tggcggatct gggaacagta caattatagt
    10381 gagcagaagt ggagaggatg aatcacaaga ggacgtgctg atggatgaag ctccttccaa
    10441 cctcagccaa gcttccacct tgcaggccaa ccgagaagat tccatgaata tcctggaccc
    10501 tgaggatgag gaggagcaca ctcaggaaga ggacagcagt ggcagtaacg aggatgagga
    10561 tgatagtcag gatgaagagg aggaggagga ggaagatgag gaagatgatc aggaggatga
    10621 tgaaggtgaa gagggagatg aagacgatga cgacgatggc tctgagatgg aattggatga
    10681 ggattatcct gatatgaacg cttctccctt ggtccgattt gagcgctttg accgggagga
    10741 tgatctcatc attgagtttg acaacatgtt ctccagtgct acagacatcc ccccatcccc
    10801 aggaaatatc cctaccaccc atccactgat ggtgcgccat gcagaccaca gttctctgac
    10861 actgggcagt ggctcttcaa caactcgtct cacccagggc atcgggcgca gtcagaggac
    10921 cctaaggcag ctgacggcca atactggcca caccattcat gttcactacc ctgggaatcg
    10981 ccagcccaac cctcctctta tactgcagag gttgcttggt ccctcagctg ctgctgacat
    11041 ccttcagctg agcagcagcc ttcccctaca aagccggggt cgggcccgcc tcctggtagg
    11101 caacgatgac gtccacatca tcgcccgttc tgatgatgag ctgctggatg actttttcca
    11161 tgatcagagc acagctacca gccaagcagg aaccctgtcc agcatcccca cagccctgac
    11221 ccgctggaca gaagaatgca aagttctcga tgctgagagc atgcatgact gtgtttcagt
    11281 ggttaaagtg tccattgtca atcacctgga attcctgagg gatgaggagc tggaagaaag
    11341 gcgagagaag cgcaggaaac aactggctga ggaagaaaca aagataactg ataaaggcaa
    11401 agaagataag gagaacaggg atcagagtgc acagtgtact gcatctaagt caaatgactc
    11461 cactgaacag aatctctcag atgggacgcc tatgcctgac agctacccaa caaccccatc
    11521 ttcaactgat gcagctacat ctgagtccaa ggagaccctt ggcactctgc aatcctcaca
    11581 acagcaacca acactcccaa ccccaccagc tttgggagag gttcctcagg agctgcagtc
    11641 tccagctgga gaagggggca gctctacaca gctattgatg cctgtagagc cagaggaatt
    11701 gggtcccaca aggccaagtg gggaagcaga aacaactcag atggagttat ccccagctcc
    11761 cactataacc tcactttccc cagagagagc tgaggattct gatgcactga cggctgtcag
    11821 cagtcagcta gaaggctctc ctatggatac aagcagcctg gcttcctgta ccttagagga
    11881 ggctgtgggt gacacttcag cagctggcag ttctgagcag cccagagcag gcagctccac
    11941 tcctggggat gccccaccag ctgtggcgga agtgcaaggc aggagtgatg ggtcagggga
    12001 atctgcccag ccacctgagg acagctcccc acctgcatcc tctgagagct cttccaccag
    12061 agattctgcc gtggccattt ctggcgccga ttcccgagga atcctagaag agccgttgcc
    12121 ttcaacaagc agtgaagaag aagatcccct tgcgggtatc agtctccctg aaggtgtgga
    12181 cccctctttt ctggctgccc tgcctgatga catccgtcgg gaagttctac agaaccagct
    12241 aggcattcgt ccaccaaccc ggactgcccc ctccacaaat agctcagcgc ctgcagtggt
    12301 ggggaatcct ggtgtgactg aggtgagtcc cgagtttctt gcggcactgc cacccgcgat
    12361 ccaagaggaa gtactggcac agcagagagc tgagcagcag cgacgagaac tagcacagaa
    12421 tgccagctca gacaccccta tggaccctgt gaccttcatc cagactctgc cctcagacct
    12481 gcgccgtagt gtcctagagg atatggagga cagtgtgtta gctgtgatgc cacctgacat
    12541 tgcagctgag gctcaagccc tgagacgaga gcaagaagcc cggcagcgac agctcatgca
    12601 tgagcgtctg tttgggcaca gtagcacctc cgcactctct gctattctcc gaagcccggc
    12661 tttcaccagt cgcttaagtg gcaaccgtgg ggtccagtat actcgccttg ctgtgcagag
    12721 aggtggcacc ttccagatgg ggggtagcag cagccataac aggccttctg gcagtaatgt
    12781 agatactctc ctccgcctcc gaggacggct ccttctggac cacgaagccc tttcttgtct
    12841 cttggtccta ctttttgtgg atgagccaaa gctcaatact agccgtctac accgagtact
    12901 gagaaatctc tgctaccatg cccagacccg ccactgggtc atccgcagtc tgctctccat
    12961 cttgcagcgc agcagtgaga gtgagctatg cattgaaaca cccaaactca ctacaagtga
    13021 ggaaaagggc aaaaagtcga gcaagagctg tgggtcaagt agccatgaga accgtcccct
    13081 ggacctgcta cacaagatgg agtcaaagag ctccaaccag ctttcctggc tctcagtatc
    13141 catggatgca gccctaggct gcaggactaa tatatttcag atccagcgtt caggggggcg
    13201 taaacatacc gagaagcatg caagcggtgg ctccaccgtc cacatccatc cccaagctgc
    13261 tcctgttgtc tgcagacacg ttttggatac actcattcaa ttggccaagg tatttcccag
    13321 ccacttcaca cagcagcgga ccaaagaaac aaactgtgag agtgatcggg aaaggggcaa
    13381 taaggcctgt agcccatgct cctcacagtc ctccagcagt ggcatttgca cagacttctg
    13441 ggacttattg gtaaaactgg acaacatgaa tgtcagccgg aaaggcaaga actccgtgaa
    13501 gtcagtgcca gtgagcgctg gcggtgaggg ggaaacctct ccatacagcc tcgaggcctc
    13561 tccactgggg cagctcatga acatgttgtc acacccagtc atccgccgga gctctctctt
    13621 aactgagaaa ctcctcagac tcctttctct catctcaatt gctctcccag aaaacaaggt
    13681 gtcagaagca caggctaatt ctggcagcgg tgcttcctcc accaccactg ccacctcaac
    13741 cacatctacc accaccacca ctgccgcctc caccacgccc acacccccta ctgcacccac
    13801 ccctgtcact tctgctccag ccctggttgc tgccacggct atttccacca ttgtcgtagc
    13861 tgcttcgacc acagtgacta cccccacgac tgctaccact actgtttcaa tttctcccac
    13921 tactaagggc agcaaatctc cagcgaaggt gagtgatggg ggcagcagca gtacagactt
    13981 taagatggtg tcctctggcc tcactgaaaa ccagctacag ctctctgtag aggtgttgac
    14041 atcccactct tgttctgagg aaggcttaga ggatgcagcc aacgtactac tgcagctctc
    14101 ccggggggac tctgggaccc gggacactgt tctcaagctg ctactgaatg gagcccgcca
    14161 tctgggttat accctttgta aacaaatagg taccctgctg gccgagctgc gggaatacaa
    14221 cctcgagcag cagcggcgag cccaatgtga aaccctctct cctgatggcc tgcctgagga
    14281 gcagccacag accaccaagc tgaagggcaa aatgcagagc aggtttgaca tggctgagaa
    14341 tgtggtaatt gtggcatctc agaagcgacc tttgggtggc cgggagctcc agctgccttc
    14401 tatgtccatg ttgacatcca agacatctac ccagaagttc ttcttgaggg tactacaggt
    14461 catcatccag ctccgggacg acacgcgccg ggctaacaag aaagccaagc agacaggcag
    14521 gctaggttcc tccggtttag gctcagctag cagcatccag gcagctgttc ggcagctgga
    14581 ggctgaggct gatgccatta tacaaatggt acgtgagggt caaagggcgc ggagacagca
    14641 acaagcagca acgtcggagt ctagccagtc agaggcgtct gtccggaggg aggaatcacc
    14701 catggatgtg gaccagccat ctcccagtgc tcaagatact caatccattg cctccgatgg
    14761 aaccccacag ggggagaagg aaaaggaaga aagaccacct gagttacccc tgctcagcga
    14821 gcagctgagt ttggacgagc tgtgggacat gcttggggag tgtctaaagg aactagagga
    14881 atcccatgac cagcatgcgg tgctagtgct acagcctgct gtcgaggcct tctttctggt
    14941 ccatgccaca gagcgggaga gcaagcctcc tgtccgagac acccgtgaga gccagctggc
    15001 acacatcaag gacgagcctc ctccactctc ccctgccccc ttaaccccag ccacgccttc
    15061 ctcccttgac ccattcttct cccgggagcc ctcatctatg cacatctcct caagcctgcc
    15121 ccctgacaca cagaagttcc ttcgctttgc agagactcac cgcactgtgt taaaccagat
    15181 cctacggcag tccacgaccc accttgctga tgggcctttt gctgtcctgg tagactacat
    15241 tcgtgtcctc gactttgatg tcaagcgcaa atatttccgc caagagctgg agcgtttaga
    15301 tgaggggctc cggaaagaag acatggctgt gcatgtccgt cgtgaccatg tgtttgaaga
    15361 ctcctatcgt gagctgcatc gcaaatcccc cgaagaaatg aagaatcgat tgtatatagt
    15421 atttgaagga gaagaagggc aggatgctgg cgggctcctg cgggagtggt atatgatcat
    15481 ctctcgagag atgtttaacc ctatgtatgc cttgttccgt acctcacctg gtgatcgagt
    15541 cacctacacc atcaatccat cttcccactg caaccccaac catctgtcct attttaaatt
    15601 tgtcggacgc attgtggcca aagctgtata tgacaaccgt cttctggagt gctactttac
    15661 tcgatccttt tacaaacaca tcttgggcaa gtcagtcaga tatacagata tggagagtga
    15721 agattaccac ttctaccaag gtctggttta tctgctggaa aatgatgtct ccacactagg
    15781 ctatgacctc accttcagca ctgaggtcca agagtttgga gtttgtgaag ttcgtgacct
    15841 caaacccaat ggggccaaca tcttggtaac agaggagaat aagaaggagt atgtacacct
    15901 ggtatgccag atgagaatga caggagccat ccgcaagcag ttggcggctt tcttagaagg
    15961 cttctatgag atcattccaa agcgcctcat ttccatcttc actgagcagg agttagagct
    16021 gcttatatca ggactgccca ccattgacat cgatgatctg aaatccaaca ctgaatacca
    16081 caagtaccag tccaactcta ttcagatcca gtggttctgg agagcattgc gttctttcga
    16141 tcaagctgac cgtgccaagt tcctccagtt tgtcacgggt acttccaagg tacccctgca
    16201 aggctttgct gccctcgaag gcatgaatgg cattcagaag tttcagatcc atcgagatga
    16261 taggtccaca gatcgcctgc cttcagctca cacatgtttt aatcagctgg atctgcctgc
    16321 ctatgagagc tttgagaagc tccgccacat gctactgttg gctatccagg agtgctctga
    16381 aggctttggg ctggcctaat aagcggccgc tgatgataat ggcctgtggc cgtaatggcc
    16441 tgtggccgta aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    16501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    16561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    16621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    16681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    16741 tccccctccc tattgccacg gcggaactca gtgccgcctg ccttgcccgc tgctggacag
    16801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    16861 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    16921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    16981 ttccgcggct tcgagatctg cctcgactgt gccttctagt tgccagccat ctgttgtttg
    17041 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    17101 aaatgaggaa attgcatcaa attgtctgag taggtgtcat tctattctgg ggggtggggt
    17161 ggggcaggac agcaaggggg aggattgggc agacaatagc aggcatgctg gggactcgag
    17221 ttaagggcga atcgctggga cgcccgaacg gtggctactc gttgacataa gcctgttcgg
    17281 ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
    17341 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
    17401 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
    17461 tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc
    17521 cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gtacatgtag gctcggccct
    17581 gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggtgatgta
    17641 gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag
    17701 acatttattg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac
    17761 gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc
    17821 ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc
    17881 aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg
    17941 gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt
    18001 accgccacct aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt
    18061 aaattgtcac aacgccgc
//
