LOCUS       sequence-369425-        7681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Corallococcus coralloides DSM2259.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: CcAvs4
FEATURES             Location/Qualifiers
     source          1..7681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     324..343
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(410..512)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(904..921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1038..1583)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1118..1137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1809..1814,1815..1832,1833..1839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             1846..1857
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      7164..7211
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(7431..7681,1..409))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
       61 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      121 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      181 tcgccatacg gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      241 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      301 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      361 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      421 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      481 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
      541 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
      601 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
      661 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
      721 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
      781 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
      841 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
      901 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
      961 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     1021 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1081 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1141 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1201 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     1261 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     1321 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     1381 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     1441 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     1501 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     1561 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     1621 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     1681 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1741 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1801 gttgcgcatt tacactttat gcttccggct cgtatgttgt gtgaaaaaga ggagaaacca
     1861 tatgcaccag gcagctcaag tatcaaggcc cgttgatttt gttattattg cggctttgga
     1921 acaggagcgg gacgcaatgt tgcgacatct gccgggctac agccggctgg accgtgagga
     1981 cgacgatccg catacctatt atgaagcgtt cgtgccgacc atgcgttccg atggtgcgcg
     2041 ataccgtgtt cttgttacca gcttagctgg catgggtccg attcaagctg cggcgaaggc
     2101 tatggcggtc gttcaccgct ggggtccgag acacgtgctg ttcgtgggca tcgccggcgg
     2161 tcgtcggtcg gtggcaggcc ttggtgacgt gctggttgct aaccaaatag cggactacac
     2221 cgtgggtaag cgtcttgaag ataggcgtat tacccgttgg gaggtgtacc gtgtggacgc
     2281 cactctcttg gacgcagcca tcaacctgcc gggtggttgg gaaagccgca cccaggaggt
     2341 gcgtccggtt cccggtcgcc cgttgaggca tagtggcatt atcctgtcgg gtggtgacgt
     2401 gctggccgat ggtgagaccc tgcgtcagaa tcaggaagta tgggataaac tgatcggcgt
     2461 cgagatggaa ggcggaggca ccgcgctagc cctgcaccag accggctccc gtccgcggtt
     2521 cctaatgatt cgtggtgtta gcgatcttgc ggacgctgag aagggtagcg ctaccgtcga
     2581 gaactggcgt ccatacgcct gcgacgtcgc cgcggcgtac acccgcgcgt tcttggagtc
     2641 cggcccggtc ccggctagtt cgggcccgag cgacggcgct ggtagcggag attggaccac
     2701 tttggccaac ctgaccacag attataaagc acacatcgag gcgtccctgc cgaacggtgt
     2761 ttctattgcg agacacgatg aagctggtaa gctggaggcc gcgctggccg agaactctat
     2821 cagtgtcgtt ttcggtgact ctggtgcggg taagtcggct ctggtcaaaa ccgttttgga
     2881 cacgcgtttt gcagcgtgga cccaggtttg gttgggtccg aaccaggttc aggcagccct
     2941 gagcgtggtg cgtcgttcca gcctgccgct gcagcatgca ctgctggatg tgctcaaagc
     3001 cagcgctaac gcacgcaacg tgctggtctt ggatagcgta gagcgtatcg aaccgtccga
     3061 aacgagcatt gttcgcaggt ttgttcatga attgctagca agcgataata gcccgcaggc
     3121 tgcttggcgc ctgatcgtga ttagccaaac ccagagctgg gcggaacgtg ctgagacaat
     3181 cctgggcgcc caaccgagcg ttctgatcga actaggcctg ttgaaaagtt ctgatgtcga
     3241 ggcggcgctg tccgccaccc cagcgctgtc ttggttagtt ggtcacccgg aaaccgttgc
     3301 ggttctggcc aaccttaaga cgcttggctg ggttattcag gcaagggctg ctctgacagc
     3361 gggcaccgtg gcccctgcct cccacgcagc ggttgccgac cgactgtggc gtttctggac
     3421 tgacggccgc gcggagattc aagagctgct tatgcgcctg gccgaacgtg aggcaagctt
     3481 tgaacgctcg tttgcactga cggacctgcg tccgggggat gcaagaacct ttcagcaggg
     3541 tcctgcgcac ctgccgctcc gcttgaatga acgtaccaat cacctggagt ttgaacatga
     3601 tctggcggct gattgggcgc gcttccagtt tctaaagcaa atcgcgcgta acaccgctcg
     3661 ctggtcggcg ctagccagca atccgctgtg gtcagctgct ttacgtatgc ttggccagtt
     3721 cctgctgcgt caaccggcgg gcaccgcgac tgcttgggat gaagcattcg ccgcggcgga
     3781 ggaaactgaa atgccgctgg ctagcgacat gctgttagat gccctttgcc tggatccgga
     3841 agcggaacgc ttcctgtacg aacgtgttga cttgttgtta gcagagaacg gtaagcgtat
     3901 gacccgtctt ttgaccagat tccatcatat aggtaccgtc ccgcgtgttc tcccaccgct
     3961 gagcgacaac ccgaccctag gccttcacat tgaaactcgt taccgcagtg taatagcggc
     4021 tcgttggcct ccaattgtac gtttcttggc gacccatagg gatcgcatta cgtctatgct
     4081 actgccggcg ttagcaaagc tggcggagac ctggctgtcc ggcaccccgc cgatgctagc
     4141 gaatggcaac ccgaccccgt tccgccgtga gttcgctgaa ttggcgattg caatggcacg
     4201 caccgtgcag gtgctcaagg aacaaggtgt tatgtttctc tcccaagatc cggtgttttt
     4261 tgcggctcca ctcgcgggag cgccagatct gcctgacgaa gtcagctgtt gggcgttaga
     4321 gatgagcggc agacggccga tctcaaaaga tgtcttgatc agagtggcgg tggcccgcaa
     4381 acgtcaagcg gaggaacgtg ctgcgcgttt gaagaacgac acccgtatgc gtgcgaccga
     4441 atcacgtcgt cgtgacctgc cgcagttggt ggtaccgggg cgccacgaac tggaaccgtg
     4501 gcctctgggt gccatgcgtc cgatcgatca tgattttcgt aacgcgtgcc tgggtggcag
     4561 ctcgctaggc ccattaatgc gtacccgtcc gacggttgcg gcagaggttc tgctggcgct
     4621 gatcatcaag gatcagccgt gtgaagaata tggtgcaggt cgttggaaaa tcaaattagg
     4681 cctggaagcg gcacgtgatg gctacccgac cgcgttctgg aaaaccccgt tcttcctgtt
     4741 cctgcaaatt gccccgcgtg aagcgcttca agcgatcatc aaactggtga atttctgcac
     4801 cgatcgctgg gtggatagtg cacggttcgg caccaaccgt tctgtgccaa cggtgaaatt
     4861 ggagatgggt aacggtattg agaaagagtt tatcggaggc ccactgctgt ttggttggac
     4921 gcaggaaaat agcatccgcg acggcaacct tcactgtgca cttgacgcgc tggagcgctg
     4981 gctgacattg caggttgaca aaaacgccga catcgcgcat tgggttgagg gtattttgaa
     5041 agaaggagcc tccgtggcgt tcttgggcct cctcgttaat ctgggtaagt atcagccggc
     5101 gctgttgccg ggtccgcttg ctccgctgct gaccagcccc ctgatgtttt actgggatgc
     5161 ccagtgcgtt gaacaagttg gcagccgttt catcggttgg cattgggcta atagcagcga
     5221 agtgatgttc gagttcgcca aatcatggac cttggcggca cacagacgtc agagattcgt
     5281 ggatatcgtg gcgaatctgc tgaaggaaga cgacgtcgtt gcggaaaagc tgcagtttgc
     5341 gatcccgaca tgggtaatcc cgaacgaccc gaaacagtct ctggagctgc gtttcatgtg
     5401 ggcagcgtta gacagggcga attatcatcg tgtcatcgac agcgaaaccg gcgccgaagt
     5461 cttcgccttg gtgtacccgg aaagcctgcg tcaggaggca aaggcatggc agaaaagcca
     5521 cgaagccgag atccaacaat tgattatccc gagccagtgc gaacagctgc tgcgtgcgca
     5581 gcaaagcttg agtgatgacg ttgcgggccg tttctatgag ctgctagagt cctctaaccg
     5641 tagcagcacc gtagaatctt ccgtaaagat gaaaagcatg ctcgctctgg ccacgacctt
     5701 ggtggtttgc ggcgcgtcct ggctgggtcg tgaaccaacg aaacgccaaa gactggtgga
     5761 aatcatccgc gctagcgcgc tcgaggtggg cgagaccgcg gttgctatcc gtacggcccg
     5821 catctccggg ggtgaggact ccctgccgtt taccgccttt gcggtgatgc acctgtggac
     5881 caaatttgat gagagcgcgg agtgggaagc gctcgtcctc cgcttactga cgtgcggtga
     5941 ctcgcgtgtt gtcgttacac tggtttccat cgcatatgct aagcgtgaga tgctaggcag
     6001 cacctggtgg cgcttcctgc aagttggtct gttttggagc gcgctggtta tgctggcacc
     6061 caactatgac gattccgagc gtatcggtcc gatttggcac ggctggctgg cgcgcattcg
     6121 tcgcttcccg ctacgtcaag agggtgcggt ttctacgggc gttaagatcg gccgcgtttt
     6181 aatgggctac cagcgtctgg agcgagcacg ttgggcgaga gcgaacggtc tgaccgtcca
     6241 gcaatgccgt ggtcgtgagt tcgacgctcg ccacagcatc ggtttagaca ttaactttct
     6301 gggtctcctt ttcaactggc tgttgaaggg tctgggcacg ggcgacctgc tgaaggaccg
     6361 cgaactggtg ctgcagctat gggattgtga agttgagtac atgcaggccc agggtgaagc
     6421 tgacggtgaa tgcagcccgc cgtcccaaca aataggttac gatttgctgg cgaagttggg
     6481 ctccttaagc gtgtcactga gtccggcgga ggcgcgtccg gtctgggagg cggtgttatc
     6541 ttgcggtccg aaagcgcact atgcccttca atattttacc cgcaacctgt ttttactcct
     6601 gaatcaaggt tgtgatgttg agcgattccg tcctctgtgg cgttctttgg ttcagtatgc
     6661 actggacgct aagtggggtg cacgtgatct gtactggccg tacggcgagc gtttgctcgg
     6721 cgacctcctg ggctttggca acgaagatct gctgaagcac ctgccttcaa aatccgtgcc
     6781 ggatatgcgt gacctgtatg atcgatgggc acaagcacac ctgggtcata gcgaggagtc
     6841 cgtgcggcgt ttctgccgtt tcctgagctc cgaggtgggg acttttctgc gtctcgacgg
     6901 tctgcgttgg attagcgcgg cgctacgtgt gagcaaaacc ggtagacaat ggtggcgcga
     6961 tgaaaatggc gacgccttgg ttgaactgct ggacaccgca ctgacgcgta atgccccggc
     7021 attggcgaac gacgcgaagg cgcgtgaagc tctggtggag ctcgctgctg agctggctgc
     7081 tcgtaacatt ccgattgcct tgaccttaca ggagagaatt cgtctgtttc gttaacattg
     7141 tgcgatctct tcagtcgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     7201 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
     7261 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     7321 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     7381 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     7441 ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca
     7501 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     7561 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     7621 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     7681 c
//
