LOCUS       sequence-369426-        7658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Escherichia coli NCTC11132.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Ec3Avs4
FEATURES             Location/Qualifiers
     source          1..7658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(397..414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(531..1076)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(611..630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      6634..6681
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6901..7560)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7475..7494
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7561..7658,1..5))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
       61 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
      121 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
      181 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
      241 ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca
      301 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
      361 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
      421 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
      481 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
      541 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      601 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
      661 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
      721 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      781 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
      841 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
      901 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
      961 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     1021 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     1081 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     1141 tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag tcatgccccg
     1201 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     1261 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc atttgttata gaatttagtg
     1321 cacatcgctt gggttaaaaa tataatgtcg caggtgtgcc aatgatagat ggccaagaaa
     1381 gcgcaaggga agaatgggcg ccaacttcga aaggaagatg catgagagat cagcaaacgc
     1441 cacccgggga cagcaaccta gggacaagca cgctggccag tgggccagca ggcacccatt
     1501 tcgaggccca agttgctgca tcgtacttgc tagccatgct cgctggggca cctgcacgag
     1561 gccttcccgg cgcatcgata gaccgagtgg ccttgcagca ggccaacgcg gggtatccgc
     1621 tcgatgacgt gatcattcac gctcacgacg gcatttctgg gaaagaggct gtcttggaag
     1681 tccaggttaa gcgatccatc accttcgcag ctgccgatcc cgtctttcgc aaagtcgtgg
     1741 gtcaaatcgt caaagcgtcg caacgacctg gcttcaccac acagcgatac gagttggcga
     1801 tcgcgacaac taaggggtcg cgcaagatcg atggggcata tcaagacgta ctcacgttcg
     1861 cccgccagag tagcgacgcg gctacgtttg ccgcccaaat tgatctagct ggggtggcga
     1921 acgacgatat gcgcgctttc gtcaagactt tcaaaactca cttgctcgac gagggctcgc
     1981 cggacgataa cgaaacagtc tggcttttgc tgcgtcgact ccaaattttg accttcgatt
     2041 ttacggcaac gggttctgca tcgcacgatc tggcgttgga acgcgccgtc cgcgttctcc
     2101 atgccgatga cggatcgcgc gccggggcct tttggagcag tctcattgag ctatcaatcg
     2161 atgtggcaaa gagcggcggc gatagaaccc gcgaaacgac actacagacg cttgcgcaac
     2221 ttggttatcg gttcgtaggt gagcgccgtc acacgatggc ccgcgccgtg ttggccgagt
     2281 catcacgctt ggttcttgcc gacattcgca accgggtagg taacgccgtc cttgcaaggc
     2341 atgagcgtgt tgccgcgaca catgcagcac tggagcgtgg gtgctatgtc gagattcgag
     2401 gagatgcagg ggtcggcaaa tcgggtgtcc tgcggcagat ggccgaagct atgcaaatcg
     2461 aggggcaggt cattgttctc agtccaggac gatgtgttcc ccgggggtgg gtcgcatggc
     2521 gtgcacagct aggcttcgac gggacacttc gcgagttgct tgtagagctg gcaaacgacg
     2581 gtggcgccac gatctttgtc gataacctcg attccttcag cgccgaagag aggctcacgg
     2641 ttgttgatgt tgtcggggaa gcgtctatcg ttccgggttt ggtcgttgta gcgacggcgc
     2701 ggagtgagtt tgggctcgat gagcccagtt ggttgccaag cgatgcgctg gcgcgcttgg
     2761 gccaaaccga accgatcacg ataggtgagt taagtaaggc agaggtcgaa caactaaaat
     2821 cgattgctcc ttcgcttgcg ccccttctca ccgatggtca tcccgcgcgt caggtcgcca
     2881 ggaacttatt tcgcctcgca cgattggcca gccagccagc atctgagcca gtgccacaca
     2941 ccgaagtaga tatggccgaa caatggtggg ccagtgcgga cggcacacgt gatgccggtt
     3001 ggcgtgatcg atctcggcta ctgcaaggcg tagcggttca gactttgtct ggcgcagact
     3061 ttctcgatat aaaaggccaa acttccgaac cgatcgatgc gctggtatcc agcggcacgt
     3121 tgcggaacct aagcatcgat ctcgtcgcat ttcgccatga cgtactgcga gagtgggcga
     3181 tcgcgaatgc cattcacgct gatcttggtc tcattgacag gctgccactt gatcggccgg
     3241 caactgcgat atttgcgcgc ggcgttgagc tggcagcgcg catggcgatc gagggggcgg
     3301 ctgatggcgt gaagtggcac gcgatcgttg ttcggctaag ccaagtagga gttcatcggt
     3361 cttggcgccg cgccgcgttg ctcgccttgg ctcgatccga agctgcggaa gcgcttctcc
     3421 aacgtgcatc gcccgagctt ctcgccaatg gtgccgcatt gctgcgcgaa ctaatccgaa
     3481 cggttatggc cgtcgacgtg cttccggctg caaaggtctt tgcggatgcg ggagtcgacc
     3541 tcgcactgat acccacatcg gtcaacgtcc cacgcggtac agcatggaca acgttgatta
     3601 tctggctctt gggtatcgca gatagtactc cgaacgaagc tattcccgag atcgtccagc
     3661 tatacgaagc gttttcgata ggtacactgg gcgttactga tatcacgccg tttacatcgc
     3721 gacagatata ccgctggctg cggctgatgg agccaaggaa cgagatgctc gcactgagtg
     3781 aaggctctgt gttttgggga caccttgatc gcgatggggt ccagtctctg aaatcggacc
     3841 ttcgcaatac tttcgtcatg tttgcgcgga caacaccgga gttggccgcc gaatacctat
     3901 tggcagtggc aaagagcgag cacagcgaag acattgtgcg tagcatcttg aagatgcgcg
     3961 gcacgctggc gcaagctgct ccggcagagc tggctgacct gaccgcacag gcgctgatcg
     4021 caacgccccg gcaacgtgag caccacttcg ctcgggcgcg cgaagaggcg tttacctatc
     4081 tcgataacga gttcctacag gtatcgccag cccaaggccc atttctcgat ttattgactc
     4141 actcgcctac ggacggcttg gccctggttc atcgcttggt tgcacacgcg gtcgcacaca
     4201 aaagccgagg agccgatcca ggaacgaata gatttaactt ggctttgccg aggggggcgt
     4261 gcgtgttccc gtggactcag acttacttct ggtcacgtaa cagcaatttc tacgccgtca
     4321 cgtcctcgct gatggcactc gaagcatggg ctcaccgccg cgtcgaggca ggcgaagcat
     4381 tcgaagatgt gctgatagac gtgcttgggc cgccgggctc gtgcgctgcg tacttactgg
     4441 tggcagttga tctcattatc tctcattggc ccaaatcgct tgatatggcg acggcctttc
     4501 tcggctgccc cgaattgctg tgcatcgatc acatgcgcca ggtgcatgat cgtgtcgaaa
     4561 taccggactt cttcaacctt aacgttttgc gtagcgagcc gcgcggcagt gtaactgccg
     4621 ctgacctgaa gcgccggcca tcccggcgga caacgctcga tgaattgatt ggtaacttta
     4681 cgttccaggc atccccagct caacatgcag cactaaggat gctgctggtg accgaggcag
     4741 cacgtttagg cgagcccgcc gcgaacgcga acctgggtaa cccggaattt atggttcggt
     4801 atgcgctgaa tctctcggat gacgcaaatt ggcaagaagt taaagtgacg ctcaaggacg
     4861 gatcatccac gagtgcgcgc gagtatgtat cacctccggc agagcaaacc catctacagg
     4921 ctttgcgcga tgccgcaacc gagcgagagt cagatttcgc cacatgctct gtgttgacac
     4981 tcgcgatcga cgatccatct cagttactgc cggaaggtcg aacagtggct gtggcttggg
     5041 cacgacgttt ggcgatggct acgacagaca ttgaatccga cgacgatgac gacgagcgtt
     5101 ggatgcaaga ggaagcgttt gtggcggccg ccatgatcgt gatgcgggat ggtaatgatg
     5161 ccttgtatgc tgaacacgga gaatgggctc ggtcgcaact gggcaagacg cttgtggcgc
     5221 tcgacgacaa tcctgtgcgt cagatgcgag ggggccttcg cttcaatccg accgcaatcg
     5281 cctatgctgg attgattcac gccttgcgac atcgatcaac aacagacgat gtgcggtcac
     5341 ttcttgaggt agcggcgagg ggaaatcacg ctgccgcgca cggcttaggg acgtccgtcg
     5401 cagtcctcgc ggccgtcgat agtcgtcttc cgagatctat tctccgatgc gcgttcaatg
     5461 cgtgtgtcgt ggataatcga gttcgggatg cgccgcagga agaggtcgaa gctcgtaagg
     5521 cgcgcagtca tgaacgggca cgggctgctg ttgccgctga gatgatatgg cttggtggag
     5581 aaggcccgga gcctatgtgg ccaatgtttc cggaagagcc agcacggccg agaaggcgac
     5641 tacgcgtgcc tgggatttgc atcgttgcac cagagctacc agaagtgtcg acgccttcac
     5701 caaccgcgca tgcagatcat caggctgcgg ctctctggct gcgacaagtg cgtggtttct
     5761 ccgatgatgc cgcaagatct tggcttagtg aagtcgtgaa atcctatatg tcgtggacga
     5821 ctgtcgcaaa tggagcaaac ctagacgacg gggacgaaac ggctcagcca ccatctgaat
     5881 ggaacgatac gttctttgcc gtggttgcgc gctgcgttgt cggcctgtca tcgcctgatg
     5941 ccatcgagtt ggttgtacgg cacatcgttg ctcttccaga ccagaatttc tatgacgtac
     6001 tcgtcgactt tttgcgtagc atcgacgcgg tgtacttcga aggccacagc attcatacgc
     6061 agacagccat tgatgttcgg agcgcacttg tagaccacat gctcactacg cgtggatggg
     6121 tacgtctcag ccaaagcaga gacttatcca tcgaaatgca ccttggcccg gcaatcgccg
     6181 ttttgttctt caacgaccac atgtacggac agggatcgaa atgctatctg tttgagaaag
     6241 gggtggagcg agttggtccg ttcctgcctg ttttgggcaa actagtcagc aaaggaccat
     6301 cacccttcat agcattggtg ttactcaact tacttgaggt tgcgccacga gccgaacaac
     6361 tgaatgtgct cgtcggggct gtaagtgtat ggcttgaggc ctatcctgat ttccaacaac
     6421 tatggatcga ctacggcgtg ggccgccgct ggtgcttggt gatggaggct atacggggac
     6481 gtagcccttc tagtttcagt agcagcgcgc cgctgcggcc tgccatcgac aacttcgtcg
     6541 catcacttgt cgcgctcggt gtacccgagg ctgcacggct ggaggaggaa ctcggcaagc
     6601 tttgaattac taacggtcaa tcggtcgcaa taactagcat aaccccttgg ggcctctaaa
     6661 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg
     6721 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     6781 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     6841 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     6901 ttacgccccg ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac
     6961 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     7021 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc
     7081 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     7141 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     7201 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     7261 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     7321 accgtctttc atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg
     7381 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     7441 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     7501 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     7561 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     7621 tatttcatta tggtgaaagt tggaacctct tacgtgcc
//
