LOCUS       sequence-369427-       17834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of FLAG-HUWE1 delta169-189.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 17834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDEST_FLAG-HUWE1_delta169-189
FEATURES             Location/Qualifiers
     source          1..17834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    995..1019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14117..14141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    14175..14797
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14374..14393)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14821..14841)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14826..15155
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15006..15141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(15193..15212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15194..15212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15226..15242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15226..15242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15239..15261)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15250..15266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15274..15280,15281..15298,15299..15304))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15319..15340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15457..15474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15628..16216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15708..15727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16387..17178,17179..17247))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17010..17029
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17248..17352)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      17379..17807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17466..17485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17676..17697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatctgat atcaacaagt ttgtacaaaa aagcaggctt
     1021 taaaggaacc aattcagtcg acatgaaagt agacaggact aaactgaaga agacacctac
     1081 tgaggctcct gcagactgca gagccttaat agacaaactc aaagtttgta atgatgagca
     1141 acttctcttg gaactgcagc agatcaaaac atggaacatt ggaaagtgcg agttatatca
     1201 ctgggtggac ctgttggacc gcttcgatgg aatactggca gatgctggac agacagtgga
     1261 gaatatgtca tggatgctcg tatgtgatag gccagaaaga gagcaactga aaatgcttct
     1321 cttggctgtg ttgaacttca cagccttgct cattgagtac agcttttccc ggcatctgta
     1381 cagttccata gagcatttga caactttatt ggcttcctct gatatgcaag tggtgctggc
     1441 agtcctcaat ctcctatatg tatttagcaa aagatcaaac tacatcactc gtctgggatc
     1501 tgacaagagg accccgctgc taactcggct acaacatttg gcagagaaat atccacccag
     1561 tgcaactaca ctacactttg aattctatgc agatcctggg gccgaggtca aaattgagaa
     1621 aaggacaact agtaacacac tacattatat tcacatagag caacttgaca agatttcaga
     1681 aagcccttct gaaatcatgg aatctcttac caaaatgtac agcattccta aggataagca
     1741 gatgctgtta tttacacaca tacgactggc ccatggcttt tctaatcaca ggaagcgatt
     1801 gcaggcagtt caggccagac tgcatgcaat atctatatta gtgtattcca atgccttgca
     1861 ggaatcagca aacagtatct tgtataatgg cttgatagag gagttagtgg acgtgctgca
     1921 aataacggat aagcagctta tggagattaa agcagcttct ttacgaacat taacatcaat
     1981 tgtccacttg gagagaactc ccaaactcag cagtattatt gactgtactg gaactgcctc
     2041 ctaccatgga tttttgccag tgcttgtaag gaactgtatc caggccatga ttgatccttc
     2101 catggatcca taccctcacc agtttgccac tgctctcttc tcttttttat accatctggc
     2161 cagctacgat gctggtggtg aagccttggt ctcctgtgga atgatggaag ccttattgaa
     2221 ggtcataaag tttcttggcg atgaacagga ccagataaca tttgtcacca gagccgtcag
     2281 agtggttgac cttatcacca acctggatat ggcagctttt caatcccata gtggactttc
     2341 tatcttcatt tatagacttg agcatgaagt agatctgtgc cgaaaagaat gtccgtttgt
     2401 gatcaagcca aagatccaga gacccaatac tacacaagaa ggagaggaaa tggaaactga
     2461 tatggatgga gtccagtgta ttccacaacg agcagcactt ctgaaatcca tgttgaattt
     2521 cctcaagaag gccatccaag accctgcttt ctcagatggc atacgacatg tgatggatgg
     2581 ttctctgcct acctccctga aacacatcat cagcaatgca gaatactatg gcccatcact
     2641 cttcctccta gctactgaag tggtgactgt gtttgtattt caagaaccat cactgctctc
     2701 ctcactccag gacaatggat tgacagatgt catgctgcat gcactgctta tcaaagatgt
     2761 tcctgctacc cgtgaagtcc ttggctccct cccaaatgta ttcagtgcac tctgtttgaa
     2821 tgcccgaggt cttcagtctt ttgttcagtg tcagcctttt gaacgcctct tcaaagttct
     2881 tctgtctcca gattacctcc cagccatgcg gaggaggaga agttctgatc cccttgggga
     2941 tactgcatcc aacctgggga gtgctgtcga tgagctcatg agacatcagc ccacccttaa
     3001 aacagatgca acgactgcca tcatcaagtt acttgaagaa atctgtaatc ttggaaggga
     3061 ccccaaatac atctgtcaga agccatcgat ccagaaggca gatggcactg ccactgctcc
     3121 tcccccaagg tctaatcatg ccgcagaaga agcctctagt gaggatgagg aggaagagga
     3181 agtacaggcc atgcagagct ttaattctac ccagcaaaat gaaactgagc ctaatcagca
     3241 ggttgttggt acagaggaac gtattcctat tcccctcatg gattacatcc ttaatgtgat
     3301 gaaatttgtg gaatctattc tgagcaacaa tacaacagat gaccactgcc aggaatttgt
     3361 gaatcagaaa ggactgttgc ctttggttac cattttgggt cttcccaatc tgcccattga
     3421 ctttcccaca tctgctgcct gtcaggctgt tgcaggtgtc tgcaaatcca tattgacact
     3481 gtcacatgaa cccaaagtcc ttcaagaggg tctccttcag ttggactcca tcctctcctc
     3541 cctggagccc ttacaccgcc ccattgaatc ccctgggggc tcagtgttgt tgcgagaact
     3601 ggcttgcgca ggcaatgttg ctgatgctac cctctcagcc caggccacac ctctgctgca
     3661 tgcactcact gctgcccatg cctacatcat gatgtttgtt catacttgca gagttggaca
     3721 gagtgaaatt cgttccatct ccgtaaacca gtggggctct caattgggtc tgagtgtttt
     3781 gagcaagctg agccagttat actgttccct ggtgtgggaa agcactgtcc tcctctctct
     3841 gtgtacccca aacagcctac catctgggtg tgaatttggc caggcagata tgcagaaact
     3901 ggttccaaag gatgagaagg caggtacgac ccagggcgga aaaagatcag atggggaaca
     3961 ggatggagca gctggaagta tggatgcttc tacccagggc ttattagaag gcattgggct
     4021 agatggtgac acattggctc ccatggagac agatgaacct actgcttcag actctaaggg
     4081 caaatctaaa atcacaccag caatggctgc cagaattaag caaatcaagc ctttgttatc
     4141 agcttcctcc agattaggcc gagcacttgc tgagctattt ggacttcttg ttaaactttg
     4201 tgtgggatct cctgtccgcc agagaaggag ccatcatgct gccagcacca ctacagcacc
     4261 gacacctgcc gcgcgatcaa cagcctcagc tctcactaag ctcttgacta aggggttatc
     4321 ttggcagccc ccaccatata cacctactcc ccgattcagg ctgacattct tcatctgttc
     4381 agttggtttc acatccccaa tgctgtttga tgagaggaag tatccctacc acctcatgct
     4441 gcaaaaattt ctctgctccg gaggccacaa tgctcttttt gaaactttca actgggctct
     4501 gtccatggga ggtaaagttc ctgtttctga gggattggaa cactcagact tgcctgatgg
     4561 cacaggagaa ttcctagatg cctggcttat gctggtggag aagatggtga atcccaccac
     4621 ggtgcttgaa tctccacatt cgctgcctgc caaattgcct ggaggtgtcc agaactttcc
     4681 ccagttcagt gcactgcgct tccttgtggt aactcagaaa gcagccttta cttgcatcaa
     4741 aaacttatgg aaccggaaac ccctgaaggt atatggtgga cgaatggctg aatcgatgct
     4801 ggccattcta tgccacatcc tccgaggaga acctgtgatt cgagagagac taagcaagga
     4861 gaaggagggg tctcgaggag aagaggatac agggcaagag gaaggtggct cccgccggga
     4921 acctcaagtc aaccagcaac aactgcaaca gctcatggac atgggcttca caagggaaca
     4981 tgcaatggag gcactgttga acaccagcac catggagcag gccacagagt accttttaac
     5041 ccaccctcct ccaatcatgg gaggagttgt tcgggatctc agcatgtctg aagaggacca
     5101 gatgatgaga gcaattgcta tgtctctggg acaggatatt ccaatggatc aaagggcaga
     5161 gtcacctgag gaagttgctt gccggaagga ggaagaggaa cggaaagctc gggaaaagca
     5221 ggaggaggaa gaggctaaat gtctagagaa gttccaggat gctgacccgt tggaacaaga
     5281 tgagctccac actttcacag atactatgtt gccaggctgc ttccaccttc ttgatgagct
     5341 gccagacaca gtataccgtg tgtgtgacct gatcatgaca gcaatcaaac gtaatggagc
     5401 agattatcgt gacatgattc tgaagcaagt agtcaatcag gtgtgggaag ctgctgatgt
     5461 attgatcaaa gctgctcttc ccctgacaac aagtgacaca aaaaccgtgt cagagtggat
     5521 aagtcagatg gccacactgc cccaggcctc caatttggct actagaatct tgcttttaac
     5581 gctacttttt gaggagttga agctaccttg tgcttgggtg gttgaatcaa gtggcatcct
     5641 taatgtccta atcaaactct tggaagtggt tcagccctgc ctccaggcag ccaaggagca
     5701 gaaggaagtc cagaccccaa agtggatcac accagtgttg ctcctgattg atttctatga
     5761 aaagacagcc atctcctcaa aaaggagagc ccagatgact aagtacctgc aatccaacag
     5821 caacaactgg cgctggtttg atgatcgctc tgggcgttgg tgtagttaca gtgcaagcaa
     5881 caatagcact attgattctg cctggaaatc tggagagaca agcgtgcgat tcactgcagg
     5941 ccgaagaaga tacacggtcc aattcactac aatggtgcag gttaatgagg aaacagggaa
     6001 ccgacgccct gtgatgctga ctctcctcag ggtacctcgg ctgaataaaa attcaaaaaa
     6061 cagcaatgga caggaactag agaagacgct ggaagaaagc aaagaaatgg atatcaaacg
     6121 taaagaaaat aaaggcaatg ataccccttt ggccctagag agtacaaaca ctgaaaagga
     6181 gacaagcctg gaggaaacaa aaatcgggga gatcctgatc cagggcttga cagaagatat
     6241 ggtgactgtt ttaatccggg cctgcgtgag catgctggga gtccctgtgg acccagatac
     6301 tttgcatgcc acccttcgtc tctgtctgag gctcacccgg gaccacaaat atgccatgat
     6361 gtttgcagaa ctgaagagta cccgcatgat cttgaatttg acccagagct caggcttcaa
     6421 tgggtttact cccctggtca cccttctctt aagacacatc attgaggacc cctgtaccct
     6481 tcgtcatacc atggaaaagg ttgttcgctc agcagctaca agtggagctg gtagcactac
     6541 ctctggtgtt gtgtctggca gcctcggctc tcgggagatc aactacatcc ttcgtgtcct
     6601 tgggccagcc gcatgccgca atccagacat attcacagaa gtggccaact gctgtatccg
     6661 catcgccctt cctgcccctc gaggctcagg aactgcttca gatgatgaat ttgagaatct
     6721 tagaattaaa ggccctaatg ctgtacagct ggtgaagacc acccctttga agccctcacc
     6781 tctgcctgtc atccctgata ctatcaagga agtgatctat gatatgctga atgctctggc
     6841 tgcataccat gctccagagg aagccgataa atctgatcct aaacctgggg ttatgaccca
     6901 agaggttggc cagctcctgc aagacatggg tgatgatgta taccagcagt accggtcact
     6961 tacgcgtcag agcagtgact ttgatacgca gtcaggtttt tccattaata gtcaggtctt
     7021 tgctgcagat ggtgcctcca ctgagacttc cgcatctggg acctcccaag gagaggcttc
     7081 aactccagag gagtctcgag atgggaagaa agataaagaa ggggaccggg cctctgagga
     7141 aggcaaacag aaaggcaagg gcagcaaacc tttaatgcct acctccacta tccttcgtct
     7201 tctggcagag ttggtgaggt cctatgttgg tattgctacc ctgattgcca actacagcta
     7261 cactgtgggc cagtctgaac tgatcaaaga ggactgcagt gtgctagctt ttgttctgga
     7321 ccacctgctc ccacataccc agaatgcaga agacaaggac acccctgcct tggcccgcct
     7381 gttcctcgca agcctggctg ctgcagggag tggcacagat gcccaggtgg ccctagtgaa
     7441 tgaagtaaaa gcagcccttg gacgggcact ggctatggct gagagtacag agaaacatgc
     7501 caggcttcag gcagtgatgt gtatcatcag tactatcatg gagtcctgcc cctccacctc
     7561 cagcttctac agcagtgcca cagcgaagac ccagcacaat ggcatgaaca acatcattcg
     7621 gcttttcctg aagaagggac tggttaatga cctggccaga gtacctcaca gcttagacct
     7681 gtccagtccc aacatggcca acacagtcaa tgctgctctg aagcctttgg aaacactttc
     7741 ccggattgtg aaccagccca gtagcctttt tggcagcaag agtgcttcta gcaagaacaa
     7801 gtctgagcag gatgcccaag gagcctctca agattccagt agcaaccagc aggacccagg
     7861 cgagcctggg gaagcagaag tgcaggagga ggatcatgat gtcactcaga cagaggtggc
     7921 agatggggat atcatggatg gggaggctga aaccgactca gtggtgattg ctgggcagcc
     7981 tgaggtgctc agttcacaag agatgcaggt tgagaatgag ctggaggacc tgatagatga
     8041 gttgcttgag agggatggcg gatctgggaa cagtacaatt atagtgagca gaagtggaga
     8101 ggatgaatca caagaggacg tgctgatgga tgaagctcct tccaacctca gccaagcttc
     8161 caccttgcag gccaaccgag aagattccat gaatatcctg gaccctgagg atgaggagga
     8221 gcacactcag gaagaggaca gcagtggcag taacgaggat gaggatgata gtcaggatga
     8281 agaggaggag gaggaggaag atgaggaaga tgatcaggag gatgatgaag gtgaagaggg
     8341 agatgaagac gatgacgacg atggctctga gatggaattg gatgaggatt atcctgatat
     8401 gaacgcttct cccttggtcc gatttgagcg ctttgaccgg gaggatgatc tcatcattga
     8461 gtttgacaac atgttctcca gtgctacaga catcccccca tccccaggaa atatccctac
     8521 cacccatcca ctgatggtgc gccatgcaga ccacagttct ctgacactgg gcagtggctc
     8581 ttcaacaact cgtctcaccc agggcatcgg gcgcagtcag aggaccctaa ggcagctgac
     8641 ggccaatact ggccacacca ttcatgttca ctaccctggg aatcgccagc ccaaccctcc
     8701 tcttatactg cagaggttgc ttggtccctc agctgctgct gacatccttc agctgagcag
     8761 cagccttccc ctacaaagcc ggggtcgggc ccgcctcctg gtaggcaacg atgacgtcca
     8821 catcatcgcc cgttctgatg atgagctgct ggatgacttt ttccatgatc agagcacagc
     8881 taccagccaa gcaggaaccc tgtccagcat ccccacagcc ctgacccgct ggacagaaga
     8941 atgcaaagtt ctcgatgctg agagcatgca tgactgtgtt tcagtggtta aagtgtccat
     9001 tgtcaatcac ctggaattcc tgagggatga ggagctggaa gaaaggcgag agaagcgcag
     9061 gaaacaactg gctgaggaag aaacaaagat aactgataaa ggcaaagaag ataaggagaa
     9121 cagggatcag agtgcacagt gtactgcatc taagtcaaat gactccactg aacagaatct
     9181 ctcagatggg acgcctatgc ctgacagcta cccaacaacc ccatcttcaa ctgatgcagc
     9241 tacatctgag tccaaggaga cccttggcac tctgcaatcc tcacaacagc aaccaacact
     9301 cccaacccca ccagctttgg gagaggttcc tcaggagctg cagtctccag ctggagaagg
     9361 gggcagctct acacagctat tgatgcctgt agagccagag gaattgggtc ccacaaggcc
     9421 aagtggggaa gcagaaacaa ctcagatgga gttatcccca gctcccacta taacctcact
     9481 ttccccagag agagctgagg attctgatgc actgacggct gtcagcagtc agctagaagg
     9541 ctctcctatg gatacaagca gcctggcttc ctgtacctta gaggaggctg tgggtgacac
     9601 ttcagcagct ggcagttctg agcagcccag agcaggcagc tccactcctg gggatgcccc
     9661 accagctgtg gcggaagtgc aaggcaggag tgatgggtca ggggaatctg cccagccacc
     9721 tgaggacagc tccccacctg catcctctga gagctcttcc accagagatt ctgccgtggc
     9781 catttctggc gccgattccc gaggaatcct agaagagccg ttgccttcaa caagcagtga
     9841 agaagaagat ccccttgcgg gtatcagtct ccctgaaggt gtggacccct cttttctggc
     9901 tgccctgcct gatgacatcc gtcgggaagt tctacagaac cagctaggca ttcgtccacc
     9961 aacccggact gccccctcca caaatagctc agcgcctgca gtggtgggga atcctggtgt
    10021 gactgaggtg agtcccgagt ttcttgcggc actgccaccc gcgatccaag aggaagtact
    10081 ggcacagcag agagctgagc agcagcgacg agaactagca cagaatgcca gctcagacac
    10141 ccctatggac cctgtgacct tcatccagac tctgccctca gacctgcgcc gtagtgtcct
    10201 agaggatatg gaggacagtg tgttagctgt gatgccacct gacattgcag ctgaggctca
    10261 agccctgaga cgagagcaag aagcccggca gcgacagctc atgcatgagc gtctgtttgg
    10321 gcacagtagc acctccgcac tctctgctat tctccgaagc ccggctttca ccagtcgctt
    10381 aagtggcaac cgtggggtcc agtatactcg ccttgctgtg cagagaggtg gcaccttcca
    10441 gatggggggt agcagcagcc ataacaggcc ttctggcagt aatgtagata ctctcctccg
    10501 cctccgagga cggctccttc tggaccacga agccctttct tgtctcttgg tcctactttt
    10561 tgtggatgag ccaaagctca atactagccg tctacaccga gtactgagaa atctctgcta
    10621 ccatgcccag acccgccact gggtcatccg cagtctgctc tccatcttgc agcgcagcag
    10681 tgagagtgag ctatgcattg aaacacccaa actcactaca agtgaggaaa agggcaaaaa
    10741 gtcgagcaag agctgtgggt caagtagcca tgagaaccgt cccctggacc tgctacacaa
    10801 gatggagtca aagagctcca accagctttc ctggctctca gtatccatgg atgcagccct
    10861 aggctgcagg actaatatat ttcagatcca gcgttcaggg gggcgtaaac ataccgagaa
    10921 gcatgcaagc ggtggctcca ccgtccacat ccatccccaa gctgctcctg ttgtctgcag
    10981 acacgttttg gatacactca ttcaattggc caaggtattt cccagccact tcacacagca
    11041 gcggaccaaa gaaacaaact gtgagagtga tcgggaaagg ggcaataagg cctgtagccc
    11101 atgctcctca cagtcctcca gcagtggcat ttgcacagac ttctgggact tattggtaaa
    11161 actggacaac atgaatgtca gccggaaagg caagaactcc gtgaagtcag tgccagtgag
    11221 cgctggcggt gagggggaaa cctctccata cagcctcgag gcctctccac tggggcagct
    11281 catgaacatg ttgtcacacc cagtcatccg ccggagctct ctcttaactg agaaactcct
    11341 cagactcctt tctctcatct caattgctct cccagaaaac aaggtgtcag aagcacaggc
    11401 taattctggc agcggtgctt cctccaccac cactgccacc tcaaccacat ctaccaccac
    11461 caccactgcc gcctccacca cgcccacacc ccctactgca cccacccctg tcacttctgc
    11521 tccagccctg gttgctgcca cggctatttc caccattgtc gtagctgctt cgaccacagt
    11581 gactaccccc acgactgcta ccactactgt ttcaatttct cccactacta agggcagcaa
    11641 atctccagcg aaggtgagtg atgggggcag cagcagtaca gactttaaga tggtgtcctc
    11701 tggcctcact gaaaaccagc tacagctctc tgtagaggtg ttgacatccc actcttgttc
    11761 tgaggaaggc ttagaggatg cagccaacgt actactgcag ctctcccggg gggactctgg
    11821 gacccgggac actgttctca agctgctact gaatggagcc cgccatctgg gttataccct
    11881 ttgtaaacaa ataggtaccc tgctggccga gctgcgggaa tacaacctcg agcagcagcg
    11941 gcgagcccaa tgtgaaaccc tctctcctga tggcctgcct gaggagcagc cacagaccac
    12001 caagctgaag ggcaaaatgc agagcaggtt tgacatggct gagaatgtgg taattgtggc
    12061 atctcagaag cgacctttgg gtggccggga gctccagctg ccttctatgt ccatgttgac
    12121 atccaagaca tctacccaga agttcttctt gagggtacta caggtcatca tccagctccg
    12181 ggacgacacg cgccgggcta acaagaaagc caagcagaca ggcaggctag gttcctccgg
    12241 tttaggctca gctagcagca tccaggcagc tgttcggcag ctggaggctg aggctgatgc
    12301 cattatacaa atggtacgtg agggtcaaag ggcgcggaga cagcaacaag cagcaacgtc
    12361 ggagtctagc cagtcagagg cgtctgtccg gagggaggaa tcacccatgg atgtggacca
    12421 gccatctccc agtgctcaag atactcaatc cattgcctcc gatggaaccc cacaggggga
    12481 gaaggaaaag gaagaaagac cacctgagtt acccctgctc agcgagcagc tgagtttgga
    12541 cgagctgtgg gacatgcttg gggagtgtct aaaggaacta gaggaatccc atgaccagca
    12601 tgcggtgcta gtgctacagc ctgctgtcga ggccttcttt ctggtccatg ccacagagcg
    12661 ggagagcaag cctcctgtcc gagacacccg tgagagccag ctggcacaca tcaaggacga
    12721 gcctcctcca ctctcccctg cccccttaac cccagccacg ccttcctccc ttgacccatt
    12781 cttctcccgg gagccctcat ctatgcacat ctcctcaagc ctgccccctg acacacagaa
    12841 gttccttcgc tttgcagaga ctcaccgcac tgtgttaaac cagatcctac ggcagtccac
    12901 gacccacctt gctgatgggc cttttgctgt cctggtagac tacattcgtg tcctcgactt
    12961 tgatgtcaag cgcaaatatt tccgccaaga gctggagcgt ttagatgagg ggctccggaa
    13021 agaagacatg gctgtgcatg tccgtcgtga ccatgtgttt gaagactcct atcgtgagct
    13081 gcatcgcaaa tcccccgaag aaatgaagaa tcgattgtat atagtatttg aaggagaaga
    13141 agggcaggat gctggcgggc tcctgcggga gtggtatatg atcatctctc gagagatgtt
    13201 taaccctatg tatgccttgt tccgtacctc acctggtgat cgagtcacct acaccatcaa
    13261 tccatcttcc cactgcaacc ccaaccatct gtcctatttt aaatttgtcg gacgcattgt
    13321 ggccaaagct gtatatgaca accgtcttct ggagtgctac tttactcgat ccttttacaa
    13381 acacatcttg ggcaagtcag tcagatatac agatatggag agtgaagatt accacttcta
    13441 ccaaggtctg gtttatctgc tggaaaatga tgtctccaca ctaggctatg acctcacctt
    13501 cagcactgag gtccaagagt ttggagtttg tgaagttcgt gacctcaaac ccaatggggc
    13561 caacatcttg gtaacagagg agaataagaa ggagtatgta cacctggtat gccagatgag
    13621 aatgacagga gccatccgca agcagttggc ggctttctta gaaggcttct atgagatcat
    13681 tccaaagcgc ctcatttcca tcttcactga gcaggagtta gagctgctta tatcaggact
    13741 gcccaccatt gacatcgatg atctgaaatc caacactgaa taccacaagt accagtccaa
    13801 ctctattcag atccagtggt tctggagagc attgcgttct ttcgatcaag ctgaccgtgc
    13861 caagttcctc cagtttgtca cgggtacttc caaggtaccc ctgcaaggct ttgctgccct
    13921 cgaaggcatg aatggcattc agaagtttca gatccatcga gatgataggt ccacagatcg
    13981 cctgccttca gctcacacat gttttaatca gctggatctg cctgcctatg agagctttga
    14041 gaagctccgc cacatgctac tgttggctat ccaggagtgc tctgaaggct ttgggctggc
    14101 ctaataagcg gccgcgaccc agctttcttg tacaaagtgg ttgatatcgg taccagtcga
    14161 ctctagagga tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
    14221 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
    14281 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
    14341 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
    14401 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
    14461 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
    14521 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
    14581 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
    14641 tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag acacagcata
    14701 ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct
    14761 catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca gcttggctgt
    14821 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    14881 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    14941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
    15001 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    15061 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    15121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc
    15181 agaaagttaa ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc
    15241 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    15301 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    15361 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    15421 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    15481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    15541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    15601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    15661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    15721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    15781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    15841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    15901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    15961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    16021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    16081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    16141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    16201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    16261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    16321 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    16381 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    16441 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    16501 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    16561 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    16621 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    16681 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    16741 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    16801 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    16861 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    16921 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    16981 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    17041 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    17101 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    17161 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    17221 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    17281 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    17341 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
    17401 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    17461 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    17521 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    17581 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    17641 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    17701 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    17761 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    17821 aacgcttaca attt
//
