LOCUS       sequence-369429-       11668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-pHluorin
FEATURES             Location/Qualifiers
     source          1..11668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2372..2570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2588..2768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2815..2940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3433..3666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3851..3895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4193..4310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4355..5643
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      5653..5662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7669..7688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    8113..8701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8166..8186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8584..8595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8704..8720)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8705..8721)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9226..9406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9432..9449)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9438..9662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9708..10136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9795..9814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10005..10026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10145..10165)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10150..10479
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10330..10465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10392..10411
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10527..10574
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10593..10967
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11097..11218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11134..11153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11188..11207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11267..11283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11267..11283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11280..11302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11291..11307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11315..11321,11322..11339,11340..11345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11360..11381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11498..11515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2581 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2641 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2701 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2761 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2821 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2881 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2941 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3001 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3061 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3121 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3181 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3241 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3301 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3361 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3421 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3481 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3541 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3601 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3661 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3721 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3781 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3841 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3901 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3961 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4021 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4081 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4141 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4201 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4261 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4321 acagggacag cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct
     4381 ccatggggtt cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca
     4441 gtgacctgcc cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa
     4501 tcttaaattc catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta
     4561 gggcagatga ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc
     4621 cgcccagccc tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc
     4681 taccttgacg ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca
     4741 tggtccctct gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc
     4801 tgctcagtgg tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg
     4861 gtctgtttgg tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa
     4921 gcagggagag ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc
     4981 ccagaggaag caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat
     5041 tagcttcccc tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc
     5101 tcttcagagg agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc
     5161 agaatgtggc acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca
     5221 tcccgtctgt taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca
     5281 cttgtggact aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg
     5341 gaacagggtc cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca
     5401 gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg
     5461 gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag
     5521 gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa
     5581 gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc
     5641 agcgggggat ccgccaccat ggaaagcgag agcatcagaa ggatgggcaa cgcttgcatc
     5701 cctctgaaga ggatcgccta cttcctgtgc ctgttcagcg tggtgctgct gaccgaggga
     5761 aagaagcctg ccaagcctaa gtgccctgcc gtgtgcacct gtagcaagga taacgccctg
     5821 tgcgagaacg cccgcagcat ccctagaaca gtgcctccag acgtgatcag cctgagcttt
     5881 gtgcggagcg gcttcaccga gatcagcgag ggatctttcc tgttcacccc tagcctgcag
     5941 ctgctgctgt tcaccagcaa cagcttcgac gtgatctccg acgacgcctt catcggcctg
     6001 cctcacctgg aatacctgtt catcgagaac aacaacatca agagcatctc caggcacacc
     6061 ttcaggggcc tgaagtccct gatccacctg agcctggcca acaacaacct gcagaccctg
     6121 cctaaggaca tcttcaaagg cctggacagc ctgaccaacg tggacctgag aggcaactcc
     6181 ttcaactgcg actgcaagct gaagtggctg gtggaatggc tgggccacac aaacgctacc
     6241 gtcgaggaca tctactgcga gggacctcca gagtacaaga agcggaagat caactctctg
     6301 agccccaagg acttcgactg catcatcacc gagttcgcca agagccagga cctgccttac
     6361 cagagcctga gcatcgacac cttctcttac ctgaacgacg agtacgtcgt gatcgcccag
     6421 ccttttaccg gcaagtgcat cttcctggaa tgggaccacg tggaaaagac cttccggaac
     6481 tacgacaaca tcaccggcac cagcaccgtc gtgtgcaagc ctatcgtgat cgacacccag
     6541 ctgtacgtga tcgtggctca gctgttcggc ggcagccaca tctacaagag agatggcttc
     6601 gccaacaagt tcatcaagat ccaggacatc gaggtgctga agatcagaaa gcccaacgac
     6661 atcgagacat tcaagatcga ggataactgg tacttcgtgg tggccgacag cagcaaggcc
     6721 ggcttcacaa caatctacaa gtggaacggc aacggcttct acagccacca gtctctgcac
     6781 gcctggtaca gagacaccga cgtcgagtac ctggaaatcg ccaggcctcc tctgacactg
     6841 agaacccctc acctgatcct gagcagctct agccagaggc ctgtgatcta ccagtggtcc
     6901 aaggccacac agctgttcat caaccagaca gacatcccca acatggaaga tgtgtacgcc
     6961 gtgaagcact tcagcgtgaa gggcgacgtg tacatctgcc tgaccagatt catcggcgac
     7021 agcaaagtga tgaagtgggg cggcagcagc tttcaggaca tccagaggat gcctagcagg
     7081 ggcagcatgg tgtttcagcc cctgcagatc aacaactacc agtacgccat cctgggcagc
     7141 gactacagct tcacccaggt gtacaattgg gacgccgaga aggccaagtt cgtgaagttc
     7201 caagagctga acgtgcaggc ccctagatct ttcacccacg tgtcaatcaa caagcggaac
     7261 ttcctgttcg ccagcagctt caagggcaac acccagatct acaagcacgt catcgtggat
     7321 ctgagcgcca gcggctctac tagtggtgga tctggtggaa ccggcggctc caaaggcgag
     7381 gaactgttta caggcgtggt gcctatcctg gtcgagctgg atggggatgt gaacggccac
     7441 aagttctccg tgtctggcga aggcgaaggg gacgccacat acggaaagct gaccctgaag
     7501 ttcatctgca ccaccggcaa gctgcctgtg ccttggccta cactggtcac cacactgaca
     7561 tacggcgtgc agtgcttcag cagatacccc gaccatatga agaggcacga cttcttcaag
     7621 agcgccatgc ctgagggcta cgtgcaagag aggaccatct tctttaagga cgacggcaac
     7681 tacaagacca gggccgaagt gaagttcgag ggcgacaccc tggtcaacag gatcgagctg
     7741 aagggaatcg acttcaaaga ggacggcaac atcctgggac acaagttgga gtacaactac
     7801 aacgatcacc aggtctacat catggccgat aagcagaaga acggcatcaa ggccaacttc
     7861 aagatccggc acaacatcga ggacggcgga gtgcagctgg ccgatcacta tcagcagaac
     7921 acccctatcg gcgacggacc tgtgctgctg cctgataacc actacctgtt taccaccagc
     7981 acactgagca aggaccccaa cgagaagagg gaccacatgg tgctgttgga gttcgtgaca
     8041 gccgccggaa tcacacacgg aatggacgag ctgtacaaat gagatatcga attcgatatc
     8101 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8701 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     8761 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8821 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8881 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8941 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     9001 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9061 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     9121 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     9181 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     9241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     9421 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9481 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9541 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9601 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     9661 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     9721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9841 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9901 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9961 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10021 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10081 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    10141 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10201 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10261 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10381 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    10441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    10501 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    10561 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    10621 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    10681 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    10741 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    10801 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    10861 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    10921 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    10981 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11041 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11101 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11161 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11221 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11281 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11341 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11401 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11461 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    11581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    11641 accgtaaaaa ggccgcgttg ctggcgtt
//
