LOCUS       sequence-36943-e        6484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Huff J, Czyz A, Landick R, Niederweis M
  TITLE     Taking phage integration to the next level as a genetic tool for 
            mycobacteria.
  JOURNAL   Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6.
  PUBMED    20692326
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2010-11-15"; volume: "468(1-2)"; pages: "8-19. Epub 2010 Aug 
            6"
COMMENT     Sequence Label: pML1335
FEATURES             Location/Qualifiers
     source          1..6484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2818..2835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2989..3577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3069..3088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5029..5062
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaacactagt cgagaaaaaa aaaagcgccg caactgcggc gctttttttt tttcaggtat
       61 ctagcttagt caatgcatat aacttcgtat aatgtatgct atacgaagtt atcccggggc
      121 gtcaggcgcc gggggcggtg tccggcggcc cccagaggaa ctgcgccagt tcctccggat
      181 cggtgaagcc ggagagatcc agcggggtct cctcgaacac ctcgaagtcg tgcaggaagg
      241 tgaaggcgag cagttcgcgg gcgaagtcct cggtccgctt ccactgcgcc ccgtcgagca
      301 gcgcggccag gatctcgcgg tcgccccgga aggcgttgag atgcagttgc accaggctgt
      361 agcgggagtc tcccgcatag acgtcggtga agtcgacgat cccggtgacc tcggtcgcgg
      421 ccaggtccac gaagatgttg gtcccgtgca ggtcgccgtg gacgaaccgg ggttcgcggc
      481 cggccagcag cgtgtccacg tccggcagcc agtcctccag gcggtccagc agccggggcg
      541 agaggtagcc ccacccgcgg tggtcctcga cggtcgccgc gcggcgttcc cgcagcagtt
      601 ccgggaagac ctcggaatgg ggggtgagca cggtgttccc ggtcagcggc accctgtgca
      661 gccggccgag cacccggccg agttcgcggg ccagggcgag cagcgcgttc cggtcggtcg
      721 tgccgtccat cgcggaccgc caggtggtgc cggtcatccg gctcatcacc aggtagggcc
      781 acggccaggc tccggtgccg ggccgcagct cgccgcggcc gaggaggcgg ggcaccggca
      841 ccggggcgtc cgccaggacc gcgtacgcct ccgactccga cgcgaggctc tccggaccgc
      901 accagtgctc gccgaacagc ttgatcaccg ggtcgggctc gccgaccagt acggggttgg
      961 tgctctcgcc gggcacccgc agcaccggcg gcaccggcag cccgagctcc tccagggctc
     1021 ggcgggccag cggctcccag aattcctggt cgttccgcag gctcgcgtag gaatcatccg
     1081 aatcaatacg gtcgagaagt aacagggatt cttgtgtcac agcggacctc tattcacagg
     1141 gtacgggccg gcttaattcc gcacggccgg tcgcgacacg gcctgtccgc accgcggatc
     1201 aggcgttgac gatgacgggc tggtcggcca cgtcggggag tcaggcaact atggatgaac
     1261 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1321 aagtttactc atatatactt tagattgatt tcgttaacat tatgggccgg tcaagatagg
     1381 tttttacccc ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc
     1441 gttgaagcct gagagttgca caggagttgc aacccggtag ccttgttcac gacggctagc
     1501 agaaaggagg atttcaacta tcttggcacg tcgcggatgg ggatcgctga agactcagcg
     1561 cagcgggagg atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct
     1621 gcagacctac gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat
     1681 cgagatggag acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat
     1741 cacgctggag gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag
     1801 ggatctgtac agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt
     1861 cacagagatg acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc
     1921 gactgcccgc cgccatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga
     1981 caagctgatc gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt
     2041 agaggcgctg acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg
     2101 gatcgcggca tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg
     2161 ccgcaaggac atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc
     2221 ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt
     2281 gacggttccg cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat
     2341 gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc
     2401 cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat
     2461 ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga
     2521 gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc
     2581 tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa
     2641 cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac
     2701 gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca
     2761 ccagccccgc cgccgccagg agcattgccg ttcccggtac ccgataccgt cggcttcctc
     2821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3721 tatatatgag taaacttggt ctgacagtta ccaatatttc tacaattatt ggattgccgt
     3781 ggtgcagggt ctcccattct cggaacccgg tgagccgtcc catgttcggg gtttccctgc
     3841 cctcgtactc accccggaac gcgacggcgt cggtccagta gcaggtctcg gagcagtacg
     3901 tcctccactg gccgtcgacc ttctcgacga actagaattg gcacatggcc cgtcacaagg
     3961 agttcgatcc ggacgtcgca ctggacaccg cgatgcgggt gttctggcgc agcggctacg
     4021 cgcacacgtc gaccgaagta ctagaaagga ggaataatga acaagggcgt gatgcggccg
     4081 ggccacgtcc agctgcgcgt gctggacatg tcgaaggccc tggagcacta cgtcgagctg
     4141 ctgggcctga tcgagatgga ccgcgacgac cagggccgcg tgtacctgaa ggcctggacc
     4201 gaggtggaca agttctcgct ggtgctgcgc gaggccgacg agccgggcat ggacttcatg
     4261 ggcttcaagg tggtggacga ggacgccctg cgccagctgg agcgcgacct gatggcctac
     4321 ggctgtgccg tggagcagct gccggccggc gagctgaact cgtgtggccg ccgcgtgcgc
     4381 ttccaggccc cgtcgggcca ccacttcgag ctgtacgccg acaaggagta caccggcaag
     4441 tggggcctga acgacgtgaa cccggaggcc tggccgcgcg acctgaaggg catggccgcc
     4501 gtgcgcttcg accacgccct gatgtacggc gacgagctgc cggccaccta cgacctgttc
     4561 accaaggtgc tgggcttcta cctggccgag caggtgctgg acgagaacgg cacccgcgtg
     4621 gcccagttcc tgtcgctgtc caccaaggcc cacgacgtgg ccttcatcca ccacccggag
     4681 aagggccggc tgcaccacgt gtcgttccac ctggagacct gggaggacct gctgcgcgcc
     4741 gccgacctga tctcgatgac cgacacctcc atcgacatcg gcccgacccg ccacggcctg
     4801 acccacggca agaccatcta cttcttcgac ccgtcgggca accgcaacga ggtgttctgc
     4861 ggcggcgact acaactaccc ggaccacaag ccggtgacct ggaccaccga ccagctgggc
     4921 aaggccatct tctaccacga ccgcatcctg aacgagcgct tcatgaccgt gctgacctga
     4981 gcggccgctc aattgagccc gcctaatggc gggctttttt ttgatatcat aacttcgtat
     5041 aatgtatgct atacgaagtt atggcgcgcc aagagtcgca caagagttgc agacccctgg
     5101 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     5161 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     5221 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     5281 agcgcgttga agcctgagag ttgcacagga gttgcaacgc gtaaaaaaaa aagccccgcg
     5341 attgcggggc tttttttttt atcgataaaa taaaaaaggg gacctctagg gtccccaatt
     5401 aattagctaa agcttattta aatctacttg tacagctcgt ccatgccgtg ggtgatgccg
     5461 gcggcggtga cgaactccag caggaccatg tggtcgcgct tctcgttcgg gtccttggac
     5521 agggcggact gggtggacag gtagtggttg tccggcagca ggaccgggcc gtcgccgatc
     5581 ggggtgttct gctggtagtg gtcggctagc tggacgccac cgtcctcgat gttgtggcgg
     5641 gtcttgaagt tggccttgat gccgttcttc tgcttgtccg cggtgatgta gacgttgtgg
     5701 gagttgtagt tgtactccag cttgtggccc aggatgttac cgtcctcctt gaagtcgatg
     5761 cccttcagct cgatgcggtt gaccagggtg tcgccctcga acttgacctc ggcacgcgtc
     5821 ttgtagttac cgtcgtcctt gaaggagatg gtgcgctcct ggacgtaacc ctccggcatg
     5881 gcggacttga agaagtcgtg gcgcttcatg tggtccgggt agcgggagaa gcactggacg
     5941 ccgtaggtca gggtggtgac cagggtcggc cacgggaccg gcagcttacc ggtggtgcag
     6001 atgaacttca gggtcagctt gccgtaggtg gcgtcgccct caccctcgcc ggagacggag
     6061 aacttgtggc cgttgacgtc accgtccagc tcgaccagga tcgggacgac gccggtgaac
     6121 agctcctcgc ccttcgacat tattaacctc ctttctgtta attaagcatg cggatcgtgc
     6181 tcatttcggg cggcgaatct ctcggcgtcg aaatgatgta tgccgtgctg gtcgtacaca
     6241 cgggtactcc cagagcctac tcgaccgagc acacgcagga caaactcgcc caccccgggt
     6301 gggttctccc gctcgtcaga gacccttctc acaccgggcc tgtgcgtgcg aacgcacaga
     6361 tcaacgccgg gatggcgtag agctcactgc gcagcaccgt ggggatgcgg ccgatcatca
     6421 cgtcgcggat cgtgccgcct cccacggccg tgacggtgcc gacgatccaa tatttctaga
     6481 gttt
//
