LOCUS       sequence-369430-        7110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Streptomyces sp. A244.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: SsAvs4
FEATURES             Location/Qualifiers
     source          1..7110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(140..145,146..163,164..170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             177..188
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4924..4971
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5191..5850)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5765..5784
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5851..5953)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6345..6362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6479..7024)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6559..6578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact
       61 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
      121 acattaattg cgttgcgcat ttacacttta tgcttccggc tcgtatgttg tgtgaaaaag
      181 aggagaaacc atatggctgg accccaagta tttataagtt atgcagacga tggtggcccg
      241 cacaccgagc aggtgagagc gctttgggag ttactgcgtg aaaacggcat tgacgcgcgc
      301 ttggacctgc cagcagccga gaggccgcag gattgggcac tatggatgca gcgtgaatat
      361 gaacaaagcg atttcgtgct ggtcgtggcg tctcaagcat ataaacgtcg tgcggagggt
      421 gcagaggcga gcggcaaggg tgaaggtgtc gcgtgggaag cgcgcctgct ccgtgccgag
      481 atctatagcc agccggattg gtatagccgt attctgcgcg tcgttctccc gggtggttca
      541 cgtgatgatc ttccatcttt tctgggcggt catccaatta cgcattacac cgttgacccg
      601 ttgaccgcag caggtgcgga gaaactgctg cgctatctga ccaatcagcc gtacgaaacc
      661 gagccgccac tcggcgccgt cccgcggttc gaaccgcgca ctgatcccag cgctcagctg
      721 gcgccttgga ctcacgcgga tccgaccacc gctgcgttca ccgactttga gcgtcagttg
      781 aatctgagaa ccccgcgtga gctttctgtg cgggcggaga acgaaacgac ccgtgtcctg
      841 gttgaccgct ccgaggcatg cgaagaactg gctggcgtga tgaccgcgac cgcggccgcg
      901 accggtactc tggtggttca tggtgaaccg tcggtcggta agagcgtgct gtgtctgcaa
      961 gctgtggacc tgttgcggga gaacggtgcg caagttgtgg ctctgagcct gagagactta
     1021 caaccgccgc tgccggcgtt agaaacggtt gtgggtaccc ccctggcgcg tctgtttggt
     1081 agcgtaccgg cagcagagtg ccgtttgctg gttgtggacg gtgcagaggc ggtccagcaa
     1141 ggctgccatc agcgcctcgt tagcctggtc gacgcagcgc acgcctccgg cctgggtgtg
     1201 gttgcggttg tcagagatga tgcgctggct gcggttgaag aagctctctg cacactgagt
     1261 ggtagcgcac tgtcacgtgc caaagttgaa cctctcccgg acgttggggt agaacaacta
     1321 ctgcatgata ttccgagcct tggtcgtttg cgtcagcgtc gcagcaggtg gctgttacgc
     1381 aggatcggct tggttggtct gctgttaaga ggcggcgtgc cggccgcgct ccgtgacggt
     1441 gctctttccg aagccgacgt gtttgacgca tgctggcgtg gttgggttcg tcgtaacgaa
     1501 cagtacccgc ctggagcacc gagcccggac gcacgtgagc aggccctctt ggacattgcc
     1561 cgcgccgagc tcggcacgga tgagggccgg ggccgcccgg atccggctgc actcccgtct
     1621 ctccgttcag acggtctgct gttgccgctg ggtccaggtg cgtcttggcg ttcgagggaa
     1681 agcttcttta atgatgtggt aagagacttc gcaaccgcgc gtttgctctt gacggacggc
     1741 tttgaatgcc tgcgcgcagc tgaggcgccg cgctgggcaa tccgtgcagc aggtgtagct
     1801 tgccaagctc gtctcgccgg tccggactct ggcggtgttg actccgcgct ggctgagctg
     1861 gttcgcctgt tcgatggctt ggctattgag catggggcac gttgggcaga cgtgccgtgg
     1921 gaagcggtgc tgtccaccgg tgcggctgaa tctgtggtgc gcgcagctac ccccatgctg
     1981 ttgggggagc acggccgtcg actgacgcag ctgttgagcg ttgcagaacg tcgtctcgcc
     2041 accttggacg ctaatgaacc gatcaccgcg gaaccgctga ttgtgtggct ggtagaacaa
     2101 ggtagcgcat tacagggtct gccgcgtgag gtggcggaga aggccgagcg cgtagttctt
     2161 aagtggctgc gtggcgttgg cctggatagc ggtgctgatg acgacccggt gacgcgagag
     2221 gttcgtcgta acgtgcgcga tgcgctattg tcccgcgacg accgcggttc ggatgaaact
     2281 ctgctcgaaa tgctggctac cctgggccca gacatcgatg acgcaacccg tactgtcttg
     2341 cggcgcgcag ctgcgcagcg tccgcgcgcc cttcaacgtg tcaccgaaac cgcggacgct
     2401 gtcttgtcgt taggtagaca cgatccggaa ctgctggcgg agttagctcg tgcgtactat
     2461 atcgcgcggc gtaaaaagcc gcgagcgcgc ggtatgctgt tcgatgacgt agtgcgcccg
     2521 catcagggcg gttggaccat tggagttccg agagcggctt ggcatcgtgg tccgttttgg
     2581 gcattgctgc acttcgagcc cgaactcggc atcgccgtca ttaacgcact gttggatcat
     2641 gccgcctgtg cacaagcgga gcaacaagcg gcgcgccatg gcgagaacac ccgtggcacc
     2701 gcagatgtgc cggccgccct cgatctgctg ggtctcggcc cgcggcacta cataggtgat
     2761 gctcaaacct acagctggta ccgtgacgct ggtaccgagc cggctccgtg tacgagcgcg
     2821 ctgcttgcgt gggaacgtgc tgccgaccgt atgcgtgccg ctggtatgag cctgcgtgat
     2881 gttgcattga cgctgctgag aggtgctcgt agcgttgcgg cggttggtgt tgttgttggt
     2941 ttcctggttc gccacttgtc cgaggttgac gacgagctgg acgacttttt ggcggtgccg
     3001 gcactttggt cattggaaac tgcacgtgct acccacgagg ccgtgggact gtatcgtatt
     3061 gcagataccc cggatgtgcg gggacgccac ctgagaaagg cctcgttccg cgacgttgcg
     3121 gcggtgctga gcgcaaaggc tgctgcagcg ggtgatgacg cggctgcgga ccgtctgcgg
     3181 gcggttggcg cccgtttgtt ggaagctgca ccgcgcgacg aagatggtag accggcgatc
     3241 ggtgctcgta tctgggcagc aagcctggat gcgtgcaatt atcggcgctt cgccgcggat
     3301 ggtcaggttg gctacgaatt taccgagccg ccggacctga ccgctgattt acacgacacc
     3361 cgtcgtgact tatcccgtac gaacaccatg tatggtctga ggaatcgtta cggtcttcac
     3421 ctgaccccgc ctttcgcttt cggtacccgt cctgttgatc cgaccgtggt tgtcgatgat
     3481 gtacgtactg ctggcgacct cctggaaaac ccgccgacgg acaccctggg cgctcacgtt
     3541 gatgctgcgg ttgctgttgc ggccgcagtt gtgcgtgcaa cgggcgctgg cacgtggatg
     3601 ccggatctag gtgatcaaca tgtattgtgg gcggtgcagg cggtgacgga agcggcctcc
     3661 acgttgcaag ccaacggcgc tgatggcgat tcaatgatta tttggggcgc tgaccgttcc
     3721 gttgcttctg cgctcccgag cctgcttctg ccgccgttta ctgataaaag cgacccggtg
     3781 gtgccaaaaa ccgagcttct agatcgcgca gcgcgtctgt tgccaacgtg catggcgaac
     3841 cacagcgacg aagtgcgtcg cgttatggca atcgcgctgc gtcgagtgtg gactgcccca
     3901 tgcggtccga cgggttcgca atgtcgtcac cgtgtggcct ggcaggctgt agaagaagca
     3961 gcgagaggca ttgccatgac cccgtatgat ggaaccggcc gtcgtgccga cctgcgcatc
     4021 aaaggtacct tgtccactgg ccttaagaaa gcgaaggcga gcgatctgaa cctgcaccgt
     4081 gtggttccgg ccctagccgc ggtcaccgat gtcgcggcaa gcgagtgctg tgttgccgcg
     4141 gacgcggaac gtctgagggc gcctctgctg gacgcttacg tgagaactgc gctgcattgg
     4201 gctcacgaag gctacgaagt tgacgacgag gagaccaccc tggtggcaga tgctctttta
     4261 gctgcgaccg cgggtggttc ggaattgcta actgacgccg ttgtggccct gggcgggatc
     4321 ggtaccgccg cggcggacct gctgcgtggc gcgtgcaccg cagccaccta caataccgag
     4381 cgtcgcgcgc gtctgcgcgc ggtgtggccg acactgctga gcgtcgtgct ggatctgcca
     4441 gatagccgtg tgagggatcg accttcctac tcggcgaccc gtgccgttgg cgcactcgtt
     4501 ccgagtccgg ccccggtttc ctacgatgaa gagatcgaca acaccgttga cactgcgcag
     4561 gatggttggg tcaccgcgtc cgagctggca gctctggttg atcgttggtt gccacgtgca
     4621 atcggtagca ggcacgcggt ggacgacctc atcggcctgc tgcgtgcatc tccgctggac
     4681 gagcagctgg ctttaggcct accgtggatc cgtgcgatcg cagttccgca gagcggtcct
     4741 accgtgcgtg acacatatct gcttgtcgat tggctgcgta gcctgcatga gagcggccgc
     4801 ctggatggcc aggcgcggcg acattacgac gtcattgtgg actccctggc agcagccggt
     4861 tctgttagcg cgcgagccct gcagcaagcg gacgagtaaa cggagttaca cctagcgcaa
     4921 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     4981 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     5041 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     5101 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     5161 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcaata
     5221 ctgttgtaat tcatttaaca ttctgccgac atggaagcca tcacagacgg catgatgaac
     5281 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatagtgaa
     5341 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     5401 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     5461 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     5521 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     5581 agggtgaaca ctatcccata tcaccagctc accgtctttc atcgccatac ggaactctgg
     5641 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     5701 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     5761 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     5821 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     5881 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     5941 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     6001 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     6061 gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
     6121 gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg
     6181 tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta
     6241 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
     6301 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
     6361 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
     6421 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
     6481 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
     6541 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
     6601 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     6661 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     6721 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6781 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
     6841 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
     6901 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
     6961 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
     7021 tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc aatttatctc
     7081 ttcaaatgta gcacctgaag tcagccccat
//
