LOCUS       sequence-369431-        7816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Archaeon isolate HyVt-335.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Avs4_Archaeon_isolate_HyVt-335
FEATURES             Location/Qualifiers
     source          1..7816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(109..211)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(603..620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(737..1282)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(817..836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1508..1513,1514..1531,1532..1538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             1545..1556
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2257..2268
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      6998..7045
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(7265..7816,1..108))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
       61 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
      121 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      181 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
      241 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
      301 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
      361 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
      421 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga
      481 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
      541 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
      601 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
      661 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
      721 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      781 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
      901 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
      961 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1021 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1081 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1141 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1201 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1261 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat
     1321 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
     1381 gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     1441 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     1501 ttgcgcattt acactttatg cttccggctc gtatgttgtg tgaaaaagag gagaaaccat
     1561 atgaaaatgg attatattaa tatactacac ctgtcggact tgcactttgg tatagagaaa
     1621 aatgttgagg aaggtgcgat aatcagccgt cgtgaagtca ccctagagag tctgctgaac
     1681 acgctcaaaa atttggataa gcattggaaa cctaatatta tcgctgtctc cggtgacatc
     1741 ggctataagg gcatcaagtc tgagtacgat gaggcgagca aatggttcga acgtctgctg
     1801 gttgcgctca acctggagcc ggatagactc ataatctgcg caggcaacca cgaccgtttc
     1861 cgctatgaga agaatccgag cgttctgcca aatactattg gtgatgctga tgagcgtctt
     1921 gccgaaatta acttcaaaaa gaattctatt ccgttctcgg actttatcga gttccagaag
     1981 acgcttaaga taccgccgct catctataaa aaaatcgcta attacctggt gggcacccgt
     2041 gaaattctgg atctgaagtt cctcattctg aacagtgcgt ggtttgcgtg gggcggcaaa
     2101 cgtgacagca acaaaatgat tatgggtaag tctcagttga tcgaaatgaa aaacgaatgc
     2161 cagattatca gccgcaccga gtttattccg gaccaaatga ccatcgcggt gctgcaccat
     2221 ccgcctgaag acctggaccc ttgtgaaatc gacaccatcg agggccgtga accgtcctac
     2281 ccgtttctgg cggaatgctc cgacctcatc ctttgcggtc acaaacacgg cgagcgcttc
     2341 ttcgctccgg ataagaagtt cgcaaaaagc tggctgttta aaacgggtgc tagctatgaa
     2401 ggtgaagaat acaagaacaa ctgtgaaatc ctgcacgtag atccgaagtc cctgtcggct
     2461 ggccgtctga aaatctacta ccagccggat aagaacaagt gggtggagaa cgtggatgag
     2521 gaaggctatt atcttttcta taccagcctg agccgtctgc ggataaacac gcaggagatc
     2581 ctggcgaaaa ttcataacaa gatcggtagc gattgcaatg tgaaccgtat cacgtatttg
     2641 aattcgattg aacaaaactt gcaggagtac aagatcattt tcattttggg tgatccgacg
     2701 gttggcaaat ctgcgttaat gaaggacgtg gccaataaga tctccgagga gtacgaggtg
     2761 attgcattta acattagcta cttcaaccac aactcgctgt cggactatct gaaggactac
     2821 aatatcgttg acgacctgcg tgatataatt aactccatca agaaggaaaa aatctgcatt
     2881 ttcatcgacc aatgtgaacg tattctggag gacgaaatta aaaaattgaa tttgttcaag
     2941 gatctgtttg tcaaattgca agaaattaac ctggagaaga aagaactcaa gttgattctg
     3001 acgtgcagaa ctgatgcgtt tcaaaaagtg tacagcgagc tgattcacag ctacgattgc
     3061 gaggaaatta aaattagcgc tgtgattatc aataacttta gcgatgaaga gctgagccag
     3121 gttttttcaa agatcccaaa cctcgaactg atctacaatc aggttcatct gaaggcgctg
     3181 atcaagaacc cgaaaatgct ggatattttg accatccgtg agtttgagat cttcccgaaa
     3241 acgaaatctg gcgagctgaa aaacattatt attaccgaaa cctacttcat ggatcagttc
     3301 tggaaacaaa ttgtgcgcaa caacgaaaag aacgcgtata acaccgtgag cccggaatcc
     3361 cgcgcgcagt tactaaacaa aatctccatc gacagcttca agaaggccga accgtataag
     3421 atcagcagcg ttatcgactt caacgttttg aagaccctga tttccgacaa tattctgatt
     3481 cggtataagg accagatttt tttcagccat gatgtgtacg aggagtggag cttgctggat
     3541 ttcattcgca acaagagcaa cattaaggac aatttcctca tcccgtataa ctcttacaag
     3601 cgtaactcaa gagcgtttca actgttctct cgttacatcc ttgaggtcga taacgattac
     3661 aaaacctgga tcagcttata ctcggaactg gaggataaca acgatgttaa cccaaattgg
     3721 atccaagaat taatcctggg cattctgaag tctgaaatcc tcctgaatca ccttaaaaat
     3781 ctgaaaatct ttctgattga taaaaactcc tcgcgcttta accagctgct gaagatactg
     3841 caactgaaat gcattacctt cgaaggtgag atcgcgtttc cgaaagattt tagttatttc
     3901 agcagcaagc cgatctaccc ggtctggtca attgttatcg actttatcct tatgaatttt
     3961 cacgaattgg gcgacgagaa cttatatgaa tgcagcgaaa tcatccgtat cttcctgaac
     4021 aatagcaacc cgcaggtgaa caaatacatc gacaaaattc tggttaacta tgaagagatc
     4081 gctaataaca cgatcctgaa tgacgagatc cagtttaatc tcgactacga gaagaagaaa
     4141 aaactgaaga aaaacgtgat ctatggtatt atcaagtgca aggcactgaa acaggacaac
     4201 aatttgacca tcattgataa tctccgcagc tcgaccaact ctaagagatt gttcaaggat
     4261 gtgatttttg atatctacgg ctgggttccg ctgtacttct cctttccgga ttataccata
     4321 aatatcctga aagacttgtt catccgcgac gagaaacgcg agaaaataat cccgaccccg
     4381 ttctcgcatc acgacttcca cttttctttc gacgagcacc cgagctttac tgagaccagc
     4441 ttcaaggtga tgctggagca aaatcaagag tgcggactga ccttcatcca taaaatcatc
     4501 aatcatgcaa tggatctttg gaaaaagatt gaagagccgg ttgttcatgg tccgggtttg
     4561 aggcgaatct cctatgaccg caccccaatc ccgcagttca tgaaaattgg cggtaaagag
     4621 attgaagtgt ggggtgatga aattgtttac aagtggatcc tgcacctggg tccgacgatc
     4681 gttaaatacg ctcttcaggc actggaagag tggattatta aacaaatcca agttaataag
     4741 gagaacagtg ctgaactgat ttacaagatc ctgtccgaca ccaatagctt cgcggtggtg
     4801 agcgtttgta gccagagcgt gctgaatatc ttcttctccg agaaaatcga tgataccaac
     4861 accctcgaga acctgatcaa ggccttaatc cctattctgg aaaagccgat tttctggatt
     4921 ttacaaggta ttttctccag cgaaaagtac atctactacc gcgacgttaa tcgtaacagc
     4981 ttcgaaaagg tgctggccta cctgctgttc ttctacacca atctgaacat catcgataag
     5041 atcacgaaac taatcgagtc cttcccgaac aacattccat ttatttttga gcaagagaaa
     5101 actgatagct tgatcgtgct ggagcgtaag gagtcgaccg acctgttggt tgcaaaaacc
     5161 aagaaagaaa actacaagcc gtttaaagtg ggtgatttca taagcatggt ctttaaggct
     5221 ccagaagacc tctacgataa ggatgagatg gaataccatg aaggctttct gaagctgcaa
     5281 agctttaagg gtaccgtatt taatgcgcta gacaacggcg gggtgattaa gaactactct
     5341 atcgaaggtc tgtatcagtt cctggaaaac tacctgaaag tctatgattt ctttagcgac
     5401 cgtcagcgtt tcgagaccta cttcgaaaag ctcaaaacgt ttctcgagac cgagtctgtt
     5461 attttgcaag agattcgtat caaagacctg ccggaggagc tgcagcagga tttcggctat
     5521 ttgaagttcc tacaaaactc taacgatatt ttggagctga ttaccggttt tttcacttta
     5581 ctgatcaact tcaagtgggg atttgttgag tctaacaaat tagaggaaaa atgtaaagaa
     5641 gtcattctga aaacactcga atataagctg aataaagatg tttatagcgc ctataacaag
     5701 aacctgatgg gtatccatca gtccgcggca aagtccctgc cgcgtctctt gtacctgtat
     5761 ccaaataaca aatctatcaa acgcgcgatt aaattgttga gcatttcccc gaaccaccaa
     5821 gttcgtgaca ttatctttac ctacctgcgt gatctgtgga ccaccaattc caattttatc
     5881 tggaagctca tcaaaaagct gcgcaaactg tccatcactc gcgcgattat tatgaacccg
     5941 gaattcatag tcattaacaa ataccgcgac ccgcgtatta cccgttccca gtttgttgat
     6001 atcaccggcc gttatattaa aaagctgtac gttaaacgta tccttcgcct tttcaaaaac
     6061 cgtctgagca aaaagcgttt tatgaacttg accccgattg acttggacct taaattgtac
     6121 cgtagctctt taaagatcat accatttaac gaagtcttaa acaaaattca accgagcaaa
     6181 aacttcatcg gctttctcaa ggacctattc tatttcacca tctacaacga cctgcagtat
     6241 aaaaaggacg gcaataatta ttctcgcatg cagacctact acggtgactg tcgcaatcac
     6301 ttctatcgtg aatggattcg tgaaagtgtg gaaatcatta cctacggcat cttccatttg
     6361 gacaataaaa acctgacgaa cgagtttttg acatttatta tctccaactg gagcaaaacc
     6421 gagaagtttt ttgagatttt cctggaagag ctgataattg cgggtaatca gtccgaattc
     6481 gagaacaaac tcatcgacat ttggtgtatg atctcggaaa gcatattcaa cactctgaag
     6541 caagagaaaa ttattaagta ctgcattatc agcctgctct tctttcgccg tccgttctat
     6601 agcaccctgc agaatcaatt ccagaatccg aatagcctca agcgcattgg tgagatcatg
     6661 cgtatctttc tggaagttaa actgtttccg gatattatct ggcttatgaa tcagattcaa
     6721 aattttgagt taaccaaatc gctgctgggg accatcagcg tgaaaatcaa gaacatcatt
     6781 tacaaggagg ataaagtgaa ggtgttgcgc aacaacacta gtattttgct gaacaattac
     6841 tggaagaagt ttcgttggga aataatctac gacgagaacc tgcacatgag ctttaaatat
     6901 ctggtggaca aaatggtcga atggaatagc ccgctggcgt ccaaactcca agatgatatg
     6961 aagttgtaaa attaattcat tatcaaatac gcaataacta gcataacccc ttggggcctc
     7021 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
     7081 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     7141 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     7201 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     7261 aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt aacattctgc
     7321 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     7381 tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat
     7441 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     7501 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     7561 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     7621 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     7681 gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg cgggcaagaa
     7741 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     7801 taatatccag ctgaac
//
