LOCUS       sequence-369432-        7121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Sulfurospirillum sp..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Avs4_Sulfurospirillum_sp.
FEATURES             Location/Qualifiers
     source          1..7121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4410..4457
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4677..5336)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5251..5270
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5337..5439)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5831..5848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5965..6510)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6045..6064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggctggttc atcgttagct ttattgtgcc gagcggtaac atggcggtga tcgttgcgct
       61 ggcgctgatc ctgattgttg cgagcatcat tcgtgacaaa gagctgagcc agagcaacac
      121 catcgaacgt ttcattcaca gcctggagga aaagaccaaa aacatcctgt ttgaaattcg
      181 caccaacatt agctatcgca atgagaagat agagctggat cgcgatgaaa ttattgaaca
      241 tattaaaaac gaactggaag aaaaacagat ttttattatt agcggcagcg gcggcgtggg
      301 caaaacgagc attatcaaaa acttgtacgt caacaaagaa ggtaattatc cgttttatat
      361 ttttaaagcg cgcgaattta aaaccaacga ttttctgttt aaccatagca ttcaagattt
      421 tattgaaagc aacaaacagc ataccaagaa aataattgtt attgatagcg cggaagcgct
      481 gctggatttt gaaagcgatc tgtttcgcga atttattagc gcgctgattc agaacaactg
      541 gaaaattatt tttaccaccc gctatagcta tctggatgat ctgaattacc acttcacgca
      601 gattctgaac attgtgccgt ttcgcattga tataaaaaat ctgagcagtg aatatctgga
      661 agcgctgagc agccgctata aatttattct gccccaagat gaaaagctgt tggatcttat
      721 caaaaacccg ttttatctga acgaatatct ggcgaactat agcaatgaag agattaatta
      781 tcaagatttc aaaaacaagt tatggaatca gcatattagc accccggata ttgaaaaatg
      841 cttttttgaa tttgcgttta aacgcgcgaa cgaaggtcag ttttatgtgg atattgattg
      901 cagcagcaac agcctgaaac agctgcagaa caaaggcatt ctgggctatg aaaaaagcct
      961 gggctatttt attacccatg atatttatga agagtgggcg cttgaaaaaa ttgtaaataa
     1021 agaatttaaa aataaagtgg acattaaaag cttttttgaa aaaataggca gcagcctgcc
     1081 gattcgccgc agctttcgca gctgggtgag cgagaaattg ctattagatg atgagaacat
     1141 caagaccttt attgaagaaa tcattgatag cgtggacatt gagatttttt ggaaagatga
     1201 aattttcatt agtattctgc tgagcgaata tagcgatacc ttttttacaa actttaaaaa
     1261 agaactgctg gaaaatgatt atgaactgct gagccgcatt agctttctgc tgcgcctggc
     1321 gtgcaaagaa gtggataaca gcttttggga aagcctgggc ctgaagaatc agcagaccaa
     1381 agaagcgaaa tatattttta ccaaaccgaa aggcaccggc tggaaaagct ttattaactt
     1441 tatttatgaa aatattgaaa ccattggcct ggcgaaaatt gatctggtgc tgccgattct
     1501 gcaagattgg aacagcaaat ttcaagaacg cagcaccacc cgcaacgcga gcctgaccgc
     1561 gctcaagtac tatgacttga ttaatcagaa ggagcataaa tatagctaca aagaaagcat
     1621 taaaaccatt tgtaaggtga ttacgagcgg cagcagcgag attaaagacg aactcagcac
     1681 catctttgat gaaatagtgg agcagaaact caaaaaccac tctgataact attacgagct
     1741 gagcaaagtg gtgctgacga gctgggatgg cctgctgatt agcaaaaacc tgccgaaaca
     1801 agtgctgaaa ctggcggatc tgttttggac caaaaccccg cgcaaagtga aaaacgacgg
     1861 catttttccg cattatgaac gcgaagaagt ggaagatgcg tttaacttaa gctcgaaata
     1921 tgagaacaag tacttcccgg cgagcgcgct gcagaccccg atttattttc tgctgaaaaa
     1981 tcacttttcc ctgaccctgg attttattct ggatcttatt aacaaaagcg tggaatatta
     2041 tgcgaaaagc ggctgggaat ataaagaaga aattcagatt gtggatgtgt ttattgatga
     2101 aaacattacc attcagcagt atcatagtca gagcctgtgg aacatttatc gcggcaacag
     2161 cagcccggtg atgccgaacc tgattcagag cattcatatg gctctggaaa agtatttact
     2221 ggaaattggc aaagtgctga aaaccgaaga tctggaattt tggctgctgt atctgctgca
     2281 gaaaagcaaa agcagcagca ttagcgcggt ggtcacgagc atagtgctgg cgaacagcga
     2341 taaagcgttt aacattgcgc gcattctgtt taaaacaaag aagtttattc aagcggattt
     2401 tcatcgccat attcaagaac agagcctgaa aagcctgtat ggcatgggct atggcctgaa
     2461 ctggcagacc aaaatttatc aagatgaacg cctgaaaacc tgcgaagata aacatcgcaa
     2521 cagcaatctg gagaacctgt ttctaaacta tcagttcttt cgcacgagcg aagtgagcga
     2581 agaagacgcg aaaaacattc aagataccct gtggggcatt ctggataact actacaaaca
     2641 actgccggat gaagaaagtc agagcgagga agataggatt tggcgcattg cgctggcgcg
     2701 cattgatcgc cgcaaaatga acattgagac cgaaaaagtg gataacggca ttcagattac
     2761 ctttaacccg aaactgagcc cggaactgca gaaatatagc caagaagcgc agaacaacag
     2821 tcagaacacc attaaatata cgagcctgta tctgtgggcg gtgaacaaaa ttgaaaacaa
     2881 ccaagattat aaaaaatata ccaactatga agaaaacccg ctgctggcgc tggaacagat
     2941 caaagaagtg attgcgattc cgcatgatca gcgcgatttt atttttcaag atgaaatatt
     3001 tcctaatgcg tccattgtcc tgctgcgcga ttatgcggaa atgctgagta gcgaagataa
     3061 agaaatttgc cgcgatatga ttctgaaatt tgcgcgcctg ccgctgagcg aaaactatca
     3121 ttatcagatt agcgatggcg tggataaagc gattaaatat ctgccgatgc tgctgattta
     3181 ttttccggaa ctgaaaaacg atattaaaat tctgctgctg ctgaacctgt tttgcgatta
     3241 tcagattggc atgagcggca aacattttta tgattttgcg attgaagcga ttaacagcta
     3301 ttttaacagc gaaagcaaca gctttattgc gatttatctg ctgctgaaac cgaaatataa
     3361 cgaactgatt acgagcaact attatggtca gaaaggcgtg agcaaacaag atctactcga
     3421 gaactttttt cagagcaacg aaaaccttct ggaaaatttc gttagcaaca aaattgtgga
     3481 atttgatatt ggcaccattg aaaccaacaa actgaaaaat ctgatagtgg tgtttaaaat
     3541 gattagcctg aacaccaccg cgaaaaaaga aaaagaactg gtgcgcaaaa ttatagaaat
     3601 tttttgcgtg aaaattttta atcagccgga tgaaaaagtg agctatgatc tgaaactgaa
     3661 atttattaac cgctttgcgc tgtttgtgct ggaagcggat aaaagcgata ttcagagcta
     3721 tctgcagccg ctgctgaatc agtttgaacc gaccgaagat attgtgtatc tgcttacgca
     3781 gtttattctg gcgcaagatg aaattaacga atatgataac ttttggcatg tgtgggaact
     3841 gtttgaagat accattattg aaacctgcaa agatggcgat aaatatcgcc atattgatca
     3901 agtgattaaa gtgtatctgc tggcgtgggg tcagtatggc gcgatttgga aagataccgc
     3961 gaaagaatgg catagcctga aagataaaga tattcgctat tttaaaaaac tgaccaaaaa
     4021 gatcggccac tgcccgagcg tgctgtatag cattagcaaa ctgctgacta gcattgcgag
     4081 caaatatcag aatgacggca tctcatgggt ggcggatctg ctgcgcaaca acagcaacct
     4141 gagcaacgat aaactggaac cggataccgt gtttcatctg gaaaacctca tccggcagta
     4201 tgtgtttatt caaggcgagc aaataaagaa agaccgcaag attaaagatg atgtgctgac
     4261 cattctgaac tttctggtgg aaaaaggcag cgcggtggcg tatatgctgc gcgaaaaagt
     4321 gctgtaatgt agttgaaaat aataatttta atgagagctt ttccaattta ggctccaggg
     4381 attggagcct ttttattatc gcgcaataac tagcataacc ccttggggcc tctaaacggg
     4441 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     4501 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
     4561 ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc
     4621 acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
     4681 gccccgccct gccactcatc gcaatactgt tgtaattcat ttaacattct gccgacatgg
     4741 aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     4801 tgcgtataat atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg
     4861 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     4921 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     4981 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     5041 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     5101 tctttcatcg ccatacggaa ctctggatga gcattcatca ggcgggcaag aatgtgaata
     5161 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     5221 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     5281 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     5341 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     5401 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
     5461 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     5521 tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg
     5581 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
     5641 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
     5701 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     5761 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     5821 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     5881 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     5941 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     6001 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6061 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     6121 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     6181 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     6241 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     6301 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     6361 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     6421 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     6481 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     6541 ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     6601 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     6661 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     6721 taattgcgtt gcgcagcgca gctgacaaag attgaccgtg agcgctctga tggagaaaga
     6781 cgatagttgc tgagtacgat atcgagggta catttctctg tgtaggggta gttatttaca
     6841 aaaaaatagg agaataatta aatgaacgag ttcatcaagt ttctgaaaaa ccagtacaag
     6901 aacttcttta ccaagatcga cgataccttc gataagttcc aaaacaccgc gatcggcaag
     6961 ttcgtgatcg aaaaactgat tctgcgtgcg aagagcttct atagcattct gagcaccttt
     7021 ggtctgattc tggtggcgca gatcagcgcg tacaacaaca tcatgcagtg gagcaacaaa
     7081 gttgacgagc aactgaacaa cagcgatggt tgggcgtacg c
//
