LOCUS       sequence-369434-        3268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs inhibitor Forsur-6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Forsur-6
FEATURES             Location/Qualifiers
     source          1..3268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(65..83)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        83..117
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             124..135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      463..510
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        576..680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(681..749,750..1541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(899..918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1712..2300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2201..2220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2454..2471
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2486..2625
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2551..2570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2711..2733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2727..2918)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ttgaagacga
       61 aagggcctcg tgatacgcct attttacggc tagctcagtc ctaggtacta tgctagctgt
      121 gaaaaagagg agaaaggatc tatggcaact atcaacgtct acaaaactat cactggctca
      181 ctggtattcg tagtgggtga actgcgtgaa gatgcaccaa tcgttggcgt gtctctgcgt
      241 gaaggggcaa ccgaggctga cgccatcgct gagattcgtc agaacattga gaacctcggc
      301 ggcctgttct tcctgctgca aggtggcagc ctgaacctcg ctaagttgca ggagcatgag
      361 cctgtactcg tgaccagctt cgaagcggaa gatgtagacg ccagtgactt cgaagccagc
      421 cgcgctgacg aagaagaagc aggcgagtaa gctgagcaat aactagcata accccttggg
      481 gcctctaaac gggtcttgag gggttttttg tataggttaa tgtcatgata ataatggttt
      541 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      901 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1201 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     1261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1381 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2401 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2461 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     2521 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2581 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     2641 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2701 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2761 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     2821 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     2881 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     2941 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     3001 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     3061 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     3121 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     3181 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     3241 gttgttgctc aggtcgcaga cgttttgc
//
