LOCUS       sequence-369436-        5654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from human herpesvirus 8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_HHV8
FEATURES             Location/Qualifiers
     source          1..5654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(124..1002)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1029..1313
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1236..1255
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3431..3458
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3477..3568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3569..3637,3638..4429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3787..3806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4600..5188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5089..5108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5342..5359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5374..5514
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5440..5459)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5600..5622
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga
       61 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
      121 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
      181 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
      241 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
      301 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
      361 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
      421 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat
      481 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
      541 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
      601 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc
      661 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
      721 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg
      781 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc
      841 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
      901 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
      961 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
     1021 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
     1081 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
     1141 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
     1201 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
     1261 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
     1321 ttttgggcta acaggaggaa ttaaccatgc tgctgagccg tcaccgtgag cgtctggcgg
     1381 cgaacctgca ggaaaccgcg aaggatgcgg gtgaacgttg ggaactgagc gcgccgacct
     1441 tcacccgtca ctgcccgaaa accgcgcgta tggcgcaccc gtttatcggt gtggttcacc
     1501 gtattaacag ctacagcagc gtgctggaga cctattgcac ccgtcaccac ccggcgaccc
     1561 cgaccagcgc gaacccggac gtgggtaccc cgcgtccgag cgaggataac gttccggcga
     1621 agccgcgtct gctggaaagc ctgagcacct acctgcaaat gcgttgcgtg cgtgaggacg
     1681 cgcacgttag caccgcggat caactggttg aataccaggc ggcgcgtaaa acccacgata
     1741 gcctgcacgc gtgcagcgtg tatcgtgagc tgcaggcgtt tctggttaac ctgagcagct
     1801 tcctgaacgg ctgctacgtg ccgggtgttc actggctgga accgttccag caacagctgg
     1861 tgatgcacac cttctttttc ctggttagca tcaaggcgcc gcaaaaaacc caccagctgt
     1921 ttggcctgtt caagcaatat tttggtctgt tcgagacccc gaacagcgtg ctgcaaacct
     1981 ttaagcagaa agcgagcgtt ttcctgatcc cgcgtcgtca cggcaagacc tggattgtgg
     2041 ttgcgatcat tagcatgctg ctggcgagcg tggaaaacat caacattggt tacgttgcgc
     2101 accagaaaca cgtggcgaac agcgttttcg cggagatcat taagaccctg tgccgttggt
     2161 ttccgccgaa aaacctgaac atcaagaaag aaaacggtac catcatttat acccgtccgg
     2221 gtggccgtag cagcagcctg atgtgcgcga cctgctttaa caaaaacagc attcgtggcc
     2281 aaaccttcaa cctgctgtat gtggacgagg cgaactttat caagaaagat gcgctgccgg
     2341 cgattctggg tttcatgctg cagaaggacg cgaaactgat ctttattagc agcgttaaca
     2401 gcagcgatcg tagcaccagc ttcctgctga acctgcgtaa cgcgcaagag aagatgctga
     2461 acgtggttag ctacgtgtgc gcggaccacc gtgaagattt tcacctgcag gacgcgctgg
     2521 ttagctgccc gtgctaccgt ctgcacatcc cgacctatat caccattgac gagagcatta
     2581 aaaccaccac caacctgttt atggaaggcg cgttcgatac cgagctgatg ggcgagggtg
     2641 cggcgagcag caacgcgacc ctgtatcgtg tggttggtga cgcggcgctg acccaattcg
     2701 acatgtgccg tgtggatacc accgcgcaac aggttcaaaa gtgcctgggc aaacagctgt
     2761 ttgtgtacat cgatccggcg tataccaaca acaccgaagc gagcggtacc ggcgttggtg
     2821 cggtggttac cagcacccag accccgaccc gtagcctgat tctgggcatg gagcactttt
     2881 tcctgcgtga cctgaccggt gcggcggcgt atgaaattgc gagctgcgcg tgcaccatga
     2941 tcaaggcgat tgcggtgctg cacccgacca ttgagcgtgt gaacgcggcg gttgaaggca
     3001 acagcagcca agacagcggt gtggcgatcg cgaccgttct gaacgagatc tgcccgctgc
     3061 cgattcactt cctgcactac accgataaga gcagcgcgct gcagtggccg atctatatgc
     3121 tgggtggcga gaaaagcagc gcgtttgaaa ccttcattta cgcgctgaac agcggtaccc
     3181 tgagcgcgag ccaaaccgtg gttagcaaca ccatcaagat tagcttcgac ccggtgacct
     3241 acctggtgga acaggttcgt gcgatcaaat gcgttccgct gcgtgatggt ggccaaagct
     3301 atagcgcgaa gcagaaacac atgagcgacg atctgctggt tgcggtggtt atggcgcact
     3361 ttatggcgac cgacgatcgt cacatgtata aaccgatcag cccgcagtaa tgccaggcat
     3421 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3781 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     3841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4741 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5341 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     5401 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5461 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     5521 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5581 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5641 aaacgcgcga ggca
//
