LOCUS       sequence-369437-        5423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage T4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_T4
FEATURES             Location/Qualifiers
     source          1..5423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    308..448
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(374..393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     534..556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(712..1590)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1617..1901
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1824..1843
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3788..3815
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3834..3925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3926..3994,3995..4786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4144..4163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4957..5423,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      361 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
      421 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
      481 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      541 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc
      601 gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct
      661 gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa
      721 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
      781 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
      841 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
      901 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
      961 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     1021 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     1081 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     1141 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     1201 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     1261 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     1321 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     1381 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     1441 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     1501 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     1561 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga
     1621 aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc
     1681 gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc
     1741 catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta
     1801 tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc
     1861 tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt ttgggctaac
     1921 aggaggaatt aaccatggaa caaccgatta atgtattaaa tgatttccat ccgttaaatg
     1981 aagctggaaa aattttaata aaacacccaa gcttagcgga aagaaaagat gaagatggaa
     2041 ttcattggat aaaatctcag tgggatggaa aatggtatcc tgaaaaattc agtgattacc
     2101 ttcgtctaca caaaatagta aaaattccaa acaactctga taagcctgaa ttatttcaaa
     2161 cttataaaga taagaataat aaaagatctc ggtatatggg tcttcctaac ttgaaacgag
     2221 ctaatattaa aacacaatgg actcgtgaaa tggttgagga atggaaaaaa tgccgagatg
     2281 atattgttta ttttgcagaa acatactgtg ccattactca tattgactat ggtgtcataa
     2341 aggttcaatt acgtgactat cagcgtgata tgctcaaaat aatgtcatct aaacgtatga
     2401 ctgtttgtaa tctatcgcgc cagctcggta aaaccaccgt agtagctatt ttccttgcac
     2461 actttgtatg ttttaacaaa gataaagctg taggtattct tgcacacaaa ggctcaatgt
     2521 ctgcggaagt tttagaccgt actaagcaag caattgaact gcttcctgac tttttacaac
     2581 caggaattgt tgaatggaat aagggttcaa ttgaactaga taatggttct tcaattggcg
     2641 cttatgcttc ctctcctgac gcagttcgtg gtaactcgtt cgcaatgatt tacattgacg
     2701 aatgtgcgtt tattccaaac ttccatgatt cctggcttgc tattcaacca gtaatttcat
     2761 ctggtcgtcg ttcgaaaatt attattacta cgactcctaa tggattaaat catttttatg
     2821 atatttggac tgctgctgtc gaaggtaaat ctggatttga accatatact gctatttgga
     2881 attcagttaa agaacgtctt tataacgatg aagatatttt tgacgatgga tggcaatgga
     2941 gcatacaaac cattaatggt tcttcattag ctcaattccg tcaagaacat actgcagcgt
     3001 ttgaagggac ttctggtaca ttaatttcag gaatgaaatt agctgttatg gattttattg
     3061 aagtaacacc agatgatcat ggttttcacc aatttaaaaa acctgaacca gatagaaaat
     3121 atattgcaac tctagattgc tcagaaggtc gtgggcaaga ttaccacgct ttgcatatta
     3181 ttgatgttac tgatgatgtg tgggaacagg ttggtgtttt gcattcaaac actatttctc
     3241 atttaattct acctgacatc gttatgcgtt atttagtaga atacaatgaa tgcccagttt
     3301 atattgaatt aaatagtact ggtgtgtcag ttgcaaaatc gctttatatg gatttagaat
     3361 acgaaggtgt tatctgcgat tcatatactg atttaggaat gaaacaaact aaacgcacga
     3421 aagcagtagg atgttccacg ctaaaagacc ttattgaaaa agataagctt attattcatc
     3481 accgagcgac tattcaagaa tttagaacgt ttagtgaaaa aggcgtgtct tgggcggctg
     3541 aagaaggtta tcatgacgat ttagtaatgt ctttagtgat ttttggatgg ttatcaacgc
     3601 agtcaaaatt tattgattat gcggataaag atgacatgcg attagcatct gaagtatttt
     3661 caaaagagct tcaggatatg agcgacgact acgcgccagt tatatttgtg gattcggttc
     3721 attctgctga gtatgttcca gtatctcatg gtatgtcaat ggtataatgc caggcatcaa
     3781 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
     3841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4141 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4441 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5101 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5401 aagcggcagg gtcggaacag gag
//
