LOCUS       sequence-369438-        5801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage CBA120.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_CBA120
FEATURES             Location/Qualifiers
     source          1..5801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2403..2430
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2449..2540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2541..2609,2610..3401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2759..2778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3572..4160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4061..4080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4314..4331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4346..4486
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4412..4431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4572..4594
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4750..5628)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(5655..5801,1..138)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
       61 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
      121 tctctactgt ttctccatac ccgttttttg ggctaacagg aggaattaac catggcttac
      181 caaaaaaggg atatagaata tgcgccgatt aaaaccggtt tcaaaatcga tgatgttaaa
      241 ttacgtatgg accaaacttt catgcgcaag ccgtctgtga gagcaccgcg tgttgaatta
      301 atgttgacgg acgagcagga ggacgagttt gtggagtgct ccatggatgc gcactacttc
      361 gcggcgaact attacaaaat caccaccatt gataaggggt tcatcctttt tgatatgcat
      421 gactaccaga aacaactgtt ccacgacttt caggattacc gcttcaacgc tgtcgtgcaa
      481 gcacgtcaga gcggcaagtg cgtcaagggt gataccttgg tgaccgttta tgataccctg
      541 tctcaggaag aattgttgct gacgatcggt gaactccact ctcgcttcga ggacgtgaat
      601 catgcagtgc cgctgaatac gattggtaat cacgacaagt tcgtcgatag ccgttttggc
      661 aagcgctact tcgttcagtc cgatagcggt tgggttccgg ttattgctgc tcacaaaacc
      721 aagaaatatg cggaatttgt catcgttact gtcacgggtc gccgtattaa cgtggcggac
      781 gaacacatgt tctttactcc agatatgaaa gagatttttg cgaaggatct gaacgccggt
      841 agctatatca tgactacgga aggcccggaa gagatccgcg agatctggca gaccggtgaa
      901 taccatcata tgtatgacct tcaagtgaaa tcctccgacc aacgctacta caccaatggc
      961 ttcctgagcc ataatacgac cgtggttgcg gcctttctac tgtggtatgc catgtttcac
     1021 tcggacaaag aaatcgccgt cctggcaaat aaagagaagc aagcgattga gatcttggat
     1081 cgtattcgta aggcgtatca agatttaccg ttctttctgc agcagggttg cgaaaaattt
     1141 ggctcgactc tgattgagtt cgagaacggt agcaaaattt atgcgtacgc tacctccagt
     1201 gactctattc gtggccgtag cgttagcctg ttgtatgttg atgaggttgc ctttatcgag
     1261 aatgacttcg aattctggga aagcaccttt ccggcgattg ctagcgcaga tacctcccgt
     1321 tgtattttga cgtctacccc gaaaggccaa cgcggactgt tctatgacat cgtgaccaag
     1381 gccgatccgc gtcatccgca gtataacgac ttccacctca ccgaggttcc gtggtataaa
     1441 gtgccggcgt acaccaagga tccggattgg gaaacgaagc aacgtgcccg tttaggtgac
     1501 gctcgtttcg atcaagaatt cgggattaaa ttccgcggtt ccgttggtag cctgataccg
     1561 gcgaaatgcc tggataagat gacctccaaa ctgtaccgtg agcccaacga gttcaccaaa
     1621 atctataaag agtacgaccc tcagcgcctg tactttggca tcgcggacac cggcaagggc
     1681 gttgaaggtg attacagcgt tctgactatc ttggatatca ccgaataccc gcatgttatt
     1741 gcggcgaagt atcgtaataa caccattcca ccgatgatgt acgcatatac tattgccgac
     1801 atgtgcaccg agtacggcga atgtccagtt ctggtggaaa ccaacaacga cgtgggtggc
     1861 caggtgatca cgatcctgta ccaagagatt gaatacccgg aaattatctt tacttctacg
     1921 gataacaaag gtaccggtaa acgcatcggc ggtcgtaaac cggaaccggg tatcaacacc
     1981 aaccgtaagg tgcgatcgat cggctgcgcg aatctgaaag cgctgatcga aaaggagatg
     2041 ctggtgatcg aggaccagga taccatcgat gagctgagca ccttcgtctt caagggcgct
     2101 cgttacgaag cagacgacgg ctgccacgac gactgtgtta tgccgctcgt gctgtacagc
     2161 tgggcagtta agcaggagtg gtttagcgac ttgaccagta ccagcattag ccaagacatg
     2221 cgtaaccgta tgtcctctac cgagagccaa caggttttcc cgtttggtgg tctggtcgta
     2281 ggcgacacac cgagcgaaac cgagcacctg ccgggttttg gcggcattca ggtgtttaac
     2341 gaaatgagcg gtatgaccat ggacgagtgg tttaagaact aatgccaggc atcaaattaa
     2401 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     2461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2761 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     2821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3061 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3541 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3601 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3661 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3721 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3781 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3841 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3901 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3961 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4021 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4081 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4141 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4201 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4261 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4321 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4381 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4441 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4501 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4561 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4621 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat
     4681 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     4741 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     4801 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     4861 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     4921 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     4981 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     5041 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     5101 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     5161 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     5221 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     5281 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     5341 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     5401 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     5461 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     5521 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     5581 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     5641 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     5701 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     5761 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa a
//
