LOCUS       sequence-369439-        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage P22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_P22
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..97
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    112..252
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(178..197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     338..360
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(516..1394)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1421..1705
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1628..1647
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3259..3286
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3305..3396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3397..3465,3466..4257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3615..3634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4428..5016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4917..4936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
       61 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
      121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
      181 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
      241 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      301 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      361 ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag
      421 cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
      481 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      541 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      601 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      661 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      721 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      781 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      841 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      901 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      961 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     1021 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
     1081 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     1141 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
     1201 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     1261 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     1321 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
     1381 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1441 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1501 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1561 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1621 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1681 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
     1741 ggaactggat gcgatcctgg acaacctgag cgatgaggaa cagattgaac tgctggagct
     1801 gctggaggaa gaggaaaact atcgtaacac ccacctgctg tacgaattcg cgccgtatag
     1861 caagcagcgt gagtttatcg acgcgggtca cgattacccg gaacgttgct tcatggcggg
     1921 caaccaactg ggtaaaagct tcaccggcgc ggcggaagtg gcgtttcacc tgaccggtcg
     1981 ttacccgggt accaagggtt atccggcgga cggcaaatac ggtggcgaat ggaagggtaa
     2041 acgtttctat gagccggtgg tgttctggat tggtggcgaa accaacgaga ccgttaccaa
     2101 gaccacccaa cgtatcctgt gcggccgtat tgaggaaaac gacgaaccgg gctacggtag
     2161 catcccgaaa gaggatatca ttagctggaa gaaaagcccg ttctttccga acctggtgga
     2221 tcacctgctg gttaagcacc acaccgcgga cggcgtggaa gatggtatca gcatttgcta
     2281 cttcaaaccg tatagccagg gtcgtgcgcg ttggcaaggc gacaccatcc acggtgtttg
     2341 gtttgatgag gaaccgccgt acagcattta tggcgagggt ctgacccgta ccaacaagta
     2401 cggccagttc agcatcctga cctttacccc gctgatgggt atgagcgacg tggttaccaa
     2461 gttcctgaaa aacccgagca agagccaaaa agtggttaac atgaccattt acgacgcgga
     2521 gcactatacc gatgaacaga aagagcaaat cattgcgagc tatccggagc atgaacgtga
     2581 agcgcgtgcg cgtggtatcc cgaccatggg cagcggtcgt atctttcaga ttccggagga
     2641 aaccatcaag tgccaaccgt tcgaatgccc ggaccacttt tatgtgattg atgcgcagga
     2701 cttcggttgg aaccacccgc aggcgcacat ccaactgtgg tgggacaaag atgcggacgt
     2761 gttttacctg gcgcgtgttt ggaagaaaag cgagaacacc gcggttcaag cgtggggcgc
     2821 ggttaagagc tgggcgaaca aaattccggt tgcgtggccg cacgacggtc accaacacga
     2881 aaagggtggc ggtgagcagc tgaaaaccca atatgcggat gcgggtttca gcatgctgcc
     2941 ggaccatgcg acctttccgg atggcggtaa cagcgtggaa agcggcatca gcgagctgcg
     3001 tgacctgatg ctggaaggtc gtttcaaggt ttttaacacc tgcgagccgt tctttgagga
     3061 attccgtctg taccaccgtg acgaaaacgg caagattgtg aaaaccaacg acgatgttct
     3121 ggatgcgacc cgttacggtt atatgatgcg tcgttttgcg cgtatgatgc gtgatatccg
     3181 taagccgaaa gagaagaaaa tcccggcgcc gattcgtccg gtgcgtcgtg gtcgttaatg
     3241 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     3301 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3601 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     3661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4561 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
//
