LOCUS       sequence-369440-        5516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage Lambda.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_Lambda
FEATURES             Location/Qualifiers
     source          1..5516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        876..1160
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1083..1102
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3140..3167
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3186..3277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3278..3346,3347..4138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3496..3515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4309..4897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4798..4817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5051..5068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5083..5223
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5149..5168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5309..5331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(5487..5516,1..849))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
       61 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
      121 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
      181 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
      241 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
      301 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
      361 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
      421 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
      481 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
      541 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
      601 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
      661 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
      721 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
      781 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
      841 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
      901 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
      961 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
     1021 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
     1081 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
     1141 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accatgaata
     1201 tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt cgctcactgt
     1261 tcaggccgga gccacagacc gccgttgaat gggcggatgc taattactat ctcccgaaag
     1321 aatccgcata ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc atcatgaatg
     1381 cgatgggcag cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt gtcggttatt
     1441 ccaaaatgct gctgggtgtt tatgcctact ttatagagca taagcagcgc aacaccctta
     1501 tctggttgcc gacggatggt gatgccgaga actttatgaa aacccacgtt gagccgacta
     1561 ttcgtgatat tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag caccgggata
     1621 acacgctcac catgaagcgt ttcactaatg ggcgtggctt ctggtgcctg ggcggtaaag
     1681 cggcaaaaaa ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa cttgctgctt
     1741 ttgatgatga tattgaacag gaaggctctc cgacgttcct gggtgacaag cgtattgaag
     1801 gctcggtctg gccaaagtcc atccgtggct ccacgccaaa agtgagaggc acctgtcaga
     1861 ttgagcgtgc agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc tgcccgcatt
     1921 gcggggagga gcagtatctt aaatttggcg acaaagagac gccgtttggc ctcaaatgga
     1981 cgccggatga cccctccagc gtgttttatc tctgcgagca taatgcctgc gtcatccgcc
     2041 agcaggagct ggactttact gatgcccgtt atatctgcga aaagaccggg atctggaccc
     2101 gtgatggcat tctctggttt tcgtcatccg gtgaagagat tgagccacct gacagtgtga
     2161 cctttcacat ctggacagcg tacagcccgt tcaccacctg ggtgcagatt gtcaaagact
     2221 ggatgaaaac gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc acgctcggtg
     2281 agacgtggga ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca gagcggaaag
     2341 agcattattc agcgcccgtt cctgaccgtg tggcttacct gaccgccggt atcgactccc
     2401 agctggaccg ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa agctggctga
     2461 ttgaccggca gattattatg ggccgccacg acgatgaaca gacgctgctg cgtgtggatg
     2521 aggccatcaa taaaacctat acccgccgga atggtgcaga aatgtcgata tcccgtatct
     2581 gctgggatac tggcgggatt gacccgacca ttgtgtatga acgctcgaaa aaacatgggc
     2641 tgttccgggt gatccccatt aaaggggcat ccgtctacgg aaagccggtg gccagcatgc
     2701 cacgtaagcg aaacaaaaac ggggtttacc ttaccgaaat cggtacggat accgcgaaag
     2761 agcagattta taaccgcttc acactgacgc cggaagggga tgaaccgctt cccggtgccg
     2821 ttcacttccc gaataacccg gatatttttg atctgaccga agcgcagcag ctgactgctg
     2881 aagagcaggt cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac agcaaaaagc
     2941 gacgcaatga ggcactcgac tgcttcgttt atgcgctggc ggcgctgcgc atcagtattt
     3001 cccgctggca gctggatctc agtgcgctgc tggcgagcct gcaggaagag gatggtgcag
     3061 caaccaacaa gaaaacactg gcagattacg cccgtgcctt atccggagag gatgaataat
     3121 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     3181 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3241 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3301 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3361 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3421 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3481 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     3541 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3601 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3661 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3721 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3781 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3841 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3901 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3961 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4021 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4081 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4141 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4201 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4441 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4921 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4981 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5041 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     5101 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     5161 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     5221 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5281 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5341 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca
     5401 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
     5461 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcac
//
