LOCUS       sequence-369441-       12025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LGI1-pHmScarlet under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 12025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-pHmScarlet
FEATURES             Location/Qualifiers
     source          1..12025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..44)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        29..358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     271..290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        406..453
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             472..846
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    976..1097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1013..1032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1067..1086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1146..1162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1146..1162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1159..1181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1170..1186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1194..1200,1201..1218,1219..1224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1239..1260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1377..1394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1548..2136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1628..1647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2307..3098,3099..3167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2930..2949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3168..3272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3347..3366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3538..3917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3919..4117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4072..4092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4135..4315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4362..4487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4980..5213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5398..5442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5740..5857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5902..7190
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      7200..7209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10017..10605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10070..10090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10488..10499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10608..10624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10609..10625)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11130..11310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11336..11353)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11342..11566
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11612..12025,1..15)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11699..11718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11909..11930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
       61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
      421 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
      481 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
      541 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
      601 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
      661 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
      721 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
      781 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
      841 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
      901 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
      961 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1081 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag aggtatcagc tcactcaaag
     1441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1981 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3301 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     3361 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     3421 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     3481 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     3541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4081 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     4141 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4201 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4261 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     4321 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     4381 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     4441 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     4501 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     4561 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     4621 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     4681 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     4741 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     4801 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     4861 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     4921 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     4981 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5041 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5101 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5161 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5221 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     5281 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     5341 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     5401 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     5461 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     5521 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     5581 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     5641 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     5701 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     5761 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     5821 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5881 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     5941 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     6001 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     6061 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     6121 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     6181 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     6241 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     6301 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     6361 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     6421 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     6481 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     6541 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     6601 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     6661 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     6721 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     6781 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     6841 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     6901 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     6961 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     7021 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     7081 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     7141 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
     7201 ccaccatgga aagcgagagc atcagaagga tgggcaacgc ttgcatccct ctgaagagga
     7261 tcgcctactt cctgtgcctg ttcagcgtgg tgctgctgac cgagggaaag aagcctgcca
     7321 agcctaagtg ccctgccgtg tgcacctgta gcaaggataa cgccctgtgc gagaacgccc
     7381 gcagcatccc tagaacagtg cctccagacg tgatcagcct gagctttgtg cggagcggct
     7441 tcaccgagat cagcgaggga tctttcctgt tcacccctag cctgcagctg ctgctgttca
     7501 ccagcaacag cttcgacgtg atctccgacg acgccttcat cggcctgcct cacctggaat
     7561 acctgttcat cgagaacaac aacatcaaga gcatctccag gcacaccttc aggggcctga
     7621 agtccctgat ccacctgagc ctggccaaca acaacctgca gaccctgcct aaggacatct
     7681 tcaaaggcct ggacagcctg accaacgtgg acctgagagg caactccttc aactgcgact
     7741 gcaagctgaa gtggctggtg gaatggctgg gccacacaaa cgctaccgtc gaggacatct
     7801 actgcgaggg acctccagag tacaagaagc ggaagatcaa ctctctgagc cccaaggact
     7861 tcgactgcat catcaccgag ttcgccaaga gccaggacct gccttaccag agcctgagca
     7921 tcgacacctt ctcttacctg aacgacgagt acgtcgtgat cgcccagcct tttaccggca
     7981 agtgcatctt cctggaatgg gaccacgtgg aaaagacctt ccggaactac gacaacatca
     8041 ccggcaccag caccgtcgtg tgcaagccta tcgtgatcga cacccagctg tacgtgatcg
     8101 tggctcagct gttcggcggc agccacatct acaagagaga tggcttcgcc aacaagttca
     8161 tcaagatcca ggacatcgag gtgctgaaga tcagaaagcc caacgacatc gagacattca
     8221 agatcgagga taactggtac ttcgtggtgg ccgacagcag caaggccggc ttcacaacaa
     8281 tctacaagtg gaacggcaac ggcttctaca gccaccagtc tctgcacgcc tggtacagag
     8341 acaccgacgt cgagtacctg gaaatcgcca ggcctcctct gacactgaga acccctcacc
     8401 tgatcctgag cagctctagc cagaggcctg tgatctacca gtggtccaag gccacacagc
     8461 tgttcatcaa ccagacagac atccccaaca tggaagatgt gtacgccgtg aagcacttca
     8521 gcgtgaaggg cgacgtgtac atctgcctga ccagattcat cggcgacagc aaagtgatga
     8581 agtggggcgg cagcagcttt caggacatcc agaggatgcc tagcaggggc agcatggtgt
     8641 ttcagcccct gcagatcaac aactaccagt acgccatcct gggcagcgac tacagcttca
     8701 cccaggtgta caattgggac gccgagaagg ccaagttcgt gaagttccaa gagctgaacg
     8761 tgcaggcccc tagatctttc acccacgtgt caatcaacaa gcggaacttc ctgttcgcca
     8821 gcagcttcaa gggcaacacc cagatctaca agcacgtcat cgtggatctg agcgccagcg
     8881 gctctactag tggtggatct ggtggaaccg gcatgtcggc taccgctgcc accgtcccgc
     8941 ctgccgcccc ggccggcgag ggtggccccc ctgcacctcc tccaaacctt actagtaaca
     9001 ggagactgca gcagacccag gcccaggtgg atgaggtggt ggacatcatg agggtgaatg
     9061 tggacaaggt cctggagcgg gaccagaagt tgtcggagct ggatgaccgt gcagatgccc
     9121 tccaggcagg ggcctcccag tttgaaacaa gtgcagccaa gctcaagcgc aaatactggt
     9181 ggaaaaacct caagatgatg atcatcttgg gagtgatctg cgccatcatc ctcatcatca
     9241 tcatcgttta cttcagcact agcggcggaa gcggcgggga tcccaccatg gtgagcaagg
     9301 gagaggccgt gatcaaggag ttcatgagat tcaaggtgca catggaggga agcatgaacg
     9361 gacacgagtt cgagatcgag ggcgagggcg agggaaggcc atacgagggg acccagacag
     9421 caaagctgaa ggtgacaaag ggcggacccc tgccttttag ctgggacatc ctgagcccac
     9481 aatttatgta tggcagcaga gcctttatta agcaccccgc cgacatcccc gactactaca
     9541 aacaatcctt tcccgagggc tttaaatggg aacgggtgat gaacttcgaa gacggcggcg
     9601 ccgtgaccgt gacccaggat accagcctgg aagacggaac cctgatctac aaagtgaagc
     9661 tcagaggcac caacttcccc cccgacggcc ccgttatgca gaagaagaca atgggttggg
     9721 aggccagctg tgagagactc taccccgagg acggcgtgct caagggcgac attttgaaag
     9781 ttctgcgcct gaaggacggc ggaagatacc tggccgactt caagaccacc tacaaggcca
     9841 aaaagcccgt gcagatgccc ggcgcataca acgtggacag acagctgacg ataaccagcc
     9901 acaacgaaga ctacaccgtg gtggagcaat acgagagaag cgaaggaagg cacagcacag
     9961 gaggcatgga tgagctgtat aaataagata tcgaattcga tatcaagctt atcgataatc
    10021 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    10081 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    10141 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    10201 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    10261 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    10321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    10381 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    10441 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    10501 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    10561 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
    10621 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
    10681 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
    10741 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10801 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
    10861 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
    10921 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
    10981 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
    11041 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
    11101 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
    11161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    11221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    11281 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
    11341 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
    11401 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
    11461 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
    11521 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
    11581 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
    11641 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    11701 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    11761 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    11821 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    11881 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    11941 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    12001 ttaaaaaatg agctgattta acaaa
//
