LOCUS       sequence-369443-       12481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ADAM23-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-ADAM23-pHluorin
FEATURES             Location/Qualifiers
     source          1..12481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2372..2570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2588..2768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2815..2940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3433..3666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3851..3895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4193..4310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4355..5643
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(6127..6146)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    8926..9514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8979..8999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9397..9408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9517..9533)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9518..9534)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10039..10219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10245..10262)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10251..10475
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10521..10949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10608..10627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10818..10839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10958..10978)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10963..11292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11143..11278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11205..11224
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11340..11387
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11406..11780
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11910..12031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11947..11966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12001..12020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12080..12096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12080..12096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12093..12115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12104..12120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12128..12134,12135..12152,12153..12158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12173..12194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12311..12328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2581 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2641 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2701 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2761 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2821 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2881 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2941 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3001 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3061 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3121 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3181 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3241 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3301 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3361 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3421 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3481 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3541 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3601 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3661 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3721 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3781 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3841 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3901 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3961 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4021 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4081 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4141 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4201 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4261 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4321 acagggacag cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct
     4381 ccatggggtt cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca
     4441 gtgacctgcc cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa
     4501 tcttaaattc catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta
     4561 gggcagatga ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc
     4621 cgcccagccc tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc
     4681 taccttgacg ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca
     4741 tggtccctct gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc
     4801 tgctcagtgg tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg
     4861 gtctgtttgg tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa
     4921 gcagggagag ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc
     4981 ccagaggaag caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat
     5041 tagcttcccc tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc
     5101 tcttcagagg agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc
     5161 agaatgtggc acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca
     5221 tcccgtctgt taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca
     5281 cttgtggact aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg
     5341 gaacagggtc cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca
     5401 gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg
     5461 gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag
     5521 gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa
     5581 gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc
     5641 agcgggggat ccgccaccat gaagcctcca ggcagctcta gtagaaggcc tcctctgacc
     5701 ggctgttctc tgcctggtgc tagtagaggc cctggcagat gtcctgctgg acctgttcct
     5761 gctagagccc ctccatgtag actgctgctg gttctgctgc tgttgccagc gctagccgcc
     5821 tctggatcta agggcgaaga actgttcacc ggcgtggtgc ccattctggt ggaactggat
     5881 ggggacgtga acggccacaa gttctccgtt tctggcgaag gcgaagggga cgccacatac
     5941 ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ttggcctaca
     6001 ctggtcacca cactgacata cggcgtgcag tgcttcagca gataccccga ccatatgaag
     6061 aggcacgact tcttcaagag cgccatgcct gagggctacg tgcaagagag gaccatcttc
     6121 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctg
     6181 gtcaacagga tcgagctgaa gggaatcgac ttcaaagagg acggcaacat cctgggacac
     6241 aagttggagt acaactacaa cgatcaccag gtgtacatca tggccgacaa gcagaagaac
     6301 ggcatcaagg ccaacttcaa gatccggcac aacatcgagg acggcggagt gcagctggct
     6361 gatcactacc agcagaacac ccctatcggc gacggacctg tgctgctgcc tgataaccac
     6421 tacctgttca ccaccagcac actgagcaag gaccccaacg agaagaggga ccacatggtg
     6481 ctgctggaat ttgtgaccgc cgctggcatc acacacggca tggatgagct ctacaagagc
     6541 ggctctacta gtggcggaag cggaggaacc ggtagcagac ctagagcccg gggggctgct
     6601 gctttgtctg ctctgcattg gaacgagaca gccgagaact ctcctggcgt ggtggccgac
     6661 gaagattcta ccctgcagca gaatggcagc agaaacacca gctacagcag cgccgtgcag
     6721 aaagagatca ccctgcctag caggctggtg tactacatca accaggacag cgagagcccc
     6781 taccacgtgc tggatacaaa ggccaggcac cagcaaaagc acaacaaggc cgtgcatctg
     6841 gcccaggcca gctttcagat cgaggccttc ggcagcaagt tcatcctgga tctgatcctg
     6901 aacaacggcc tgctgagcag cgactacgtg gaaatccact acgaggacgg aaagcagatg
     6961 tacagcaaag gcggcgagca ctgctactac cacggctcta tcagaggcgt gaaggacagc
     7021 agagtggccc tgtctacatg caacggactg cacggcatgt tcgaggacga taccttcgtg
     7081 tacatgatcg agcccctgga actgacccac gacgagaagt ctacaggcag gccccacatc
     7141 atccagaaaa ccctggccgg acagtacagc aagcagatga agaacctgag caccgacggc
     7201 agcgatcagt ggcctctgtt gcctgaactg caatggctga gaaggcggaa gagggctgtg
     7261 aacccttcta ggggcgtgtt cgaagagatg aagtacttgg agctgatgat cgtcaacgac
     7321 cacaagacct acaagaagca cagaagctct cacgcccaca ccaacaactt cgccaagagc
     7381 gtggtcaacc tggtggacag catctacaaa gagcagctga acaccagagt ggtgctggtc
     7441 gccgtggaaa cctggacaga gaaggaccag atcgacatca caatcaaccc cgtgcagatg
     7501 ctgcatgagt tcagcaagta caggcagagg atcaagcagc acgccgacgc tgtgcacctg
     7561 atctccagag tgaccttcca ctacaagagg tccagcctga gctacttcgg cggcgtgtgt
     7621 agcagagtga aaggcgtggg agtgaacgag tacggcctgc ctatggctgt ggctcaggtg
     7681 ctgtctcaga gcctggctca gaacctggga atccagtggg agcccagctc cagaaagcct
     7741 aagtgcgagt gcatcgagag ctggggcggc tgcatcatgg aagaaacagg cgtgtcccac
     7801 agccggaagt tctccaagtg ctccatcctg gaataccggg acttcctgca aagaggcggc
     7861 ggagcctgtc tgttcaacag gcctacaaag ctgttcgagc ccaccgagtg tggcaacgga
     7921 tatgtggaag ccggcgagga atgcgactgt ggctttcacg tggaatgcta cggcgtgtgc
     7981 tgcaagaagt gcagcctgtc taacggcgcc cactgttctg acggcccttg ctgtaacaac
     8041 acctcctgcc tgttccagag caggggctac gagtgtaggg acgccgtgaa ctcttgcgac
     8101 atcaccgagt actgtaccgg cgactctggc cagtgtcctc ctaacctgca caagcaggac
     8161 ggctacgcct gcaaccagaa ccagggcaga tgttacaacg gcgagtgcaa gaccagagac
     8221 aaccagtgcc agtacatctg gggcaccaag gcctctggct ccgacaagtt ctgctacgag
     8281 aagctgaata ccgagggcac cgagaagggc aactgtggca aggatggcga cagatggatt
     8341 cagtgctcca agcacgacgt gttctgcggc ttcctgctgt gtaccaacct gaccagggct
     8401 cctagaatcg gacagctgca gggcgagatc atccccacca gcttttacca ccaaggcaga
     8461 gtgatcgact gctctggcgc tcacgtggtc ctggacgacg atacagatgt gggctacgtc
     8521 gaggatggca ccccttgtgg ccctagcatg atgtgtctgg acagaaagtg cctgcagatt
     8581 caggccctga atatgagcag ctgccctctg gatagcaagg gcaaagtgtg ctctggccat
     8641 ggcgtgtgtt ccaacgaggc cacctgtatc tgcgacttca catgggccgg caccgactgt
     8701 agcatcagag atcctgtgcg gaaccccaat cctcctaagg acgagggacc caaagtgaac
     8761 atggccacct ccagactgat cggagccgtg gctggaacag tgctggctct gggagtgatc
     8821 ttcggcggta caggatgggg catcgagaac gtgaagaaga gaagattcga ccccactcag
     8881 cagggaccca tctgagatat cgaattcgat atcaagctta tcgataatca acctctggat
     8941 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9001 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9061 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     9121 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     9181 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     9241 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     9301 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     9361 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9421 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9481 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     9541 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     9601 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     9661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9721 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     9781 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     9841 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     9901 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     9961 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
    10021 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10081 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10141 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10201 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
    10261 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    10321 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    10381 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    10441 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    10501 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    10801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    10861 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    10921 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    10981 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    11041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    11101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    11161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    11221 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    11281 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
    11341 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
    11401 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
    11461 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
    11521 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
    11581 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
    11641 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
    11701 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
    11761 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
    11821 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    11881 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    11941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    12061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    12181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    12241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    12301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    12361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    12421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    12481 t
//
