LOCUS       sequence-369445-       11656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant S473L LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-S473L-pHluorin
FEATURES             Location/Qualifiers
     source          1..11656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2365..3653
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(5673..5692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6111..6699
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6164..6184)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6582..6593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6702..6718)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6703..6719)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7224..7404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7430..7447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7436..7660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7706..8134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7793..7812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8003..8024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8143..8163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8148..8477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8328..8463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8390..8409
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8525..8572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8591..8965
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9095..9216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9132..9151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9186..9205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9265..9281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9265..9281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9278..9300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9289..9305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9313..9319,9320..9337,9338..9343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9358..9379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9496..9513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9667..10255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9747..9766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10426..11217,11218..11286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11049..11068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11287..11391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11466..11485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct ccatggggtt
     2401 cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca gtgacctgcc
     2461 cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa tcttaaattc
     2521 catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta gggcagatga
     2581 ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc cgcccagccc
     2641 tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc taccttgacg
     2701 ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca tggtccctct
     2761 gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg
     2821 tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg gtctgtttgg
     2881 tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa gcagggagag
     2941 ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc ccagaggaag
     3001 caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat tagcttcccc
     3061 tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc tcttcagagg
     3121 agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc agaatgtggc
     3181 acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca tcccgtctgt
     3241 taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca cttgtggact
     3301 aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg gaacagggtc
     3361 cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca gggcaaagag
     3421 gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg gggcaacggg
     3481 aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag gctcgcgagg
     3541 ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa gccggttctc
     3601 cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc agcgggggat
     3661 ccatggaaag cgagagcatc agaaggatgg gcaacgcttg catccctctg aagaggatcg
     3721 cctacttcct gtgcctgttc agcgtggtgc tgctgaccga gggaaagaag cctgccaagc
     3781 ctaagtgccc tgccgtgtgc acctgtagca aggataacgc cctgtgcgag aacgcccgca
     3841 gcatccctag aacagtgcct ccagacgtga tcagcctgag ctttgtgcgg agcggcttca
     3901 ccgagatcag cgagggatct ttcctgttca cccctagcct gcagctgctg ctgttcacca
     3961 gcaacagctt cgacgtgatc tccgacgacg ccttcatcgg cctgcctcac ctggaatacc
     4021 tgttcatcga gaacaacaac atcaagagca tctccaggca caccttcagg ggcctgaagt
     4081 ccctgatcca cctgagcctg gccaacaaca acctgcagac cctgcctaag gacatcttca
     4141 aaggcctgga cagcctgacc aacgtggacc tgagaggcaa ctccttcaac tgcgactgca
     4201 agctgaagtg gctggtggaa tggctgggcc acacaaacgc taccgtcgag gacatctact
     4261 gcgagggacc tccagagtac aagaagcgga agatcaactc tctgagcccc aaggacttcg
     4321 actgcatcat caccgagttc gccaagagcc aggacctgcc ttaccagagc ctgagcatcg
     4381 acaccttctc ttacctgaac gacgagtacg tcgtgatcgc ccagcctttt accggcaagt
     4441 gcatcttcct ggaatgggac cacgtggaaa agaccttccg gaactacgac aacatcaccg
     4501 gcaccagcac cgtcgtgtgc aagcctatcg tgatcgacac ccagctgtac gtgatcgtgg
     4561 ctcagctgtt cggcggcagc cacatctaca agagagatgg cttcgccaac aagttcatca
     4621 agatccagga catcgaggtg ctgaagatca gaaagcccaa cgacatcgag acattcaaga
     4681 tcgaggataa ctggtacttc gtggtggccg acagcagcaa ggccggcttc acaacaatct
     4741 acaagtggaa cggcaacggc ttctacagcc accagtctct gcacgcctgg tacagagaca
     4801 ccgacgtcga gtacctggaa atcgccaggc ctcctctgac actgagaacc cctcacctga
     4861 tcctgagcag ctctagccag aggcctgtga tctaccagtg gtccaaggcc acacagctgt
     4921 tcatcaacca gacagacatc cccaacatgg aagatgtgta cgccgtgaag cacttcagcg
     4981 tgaagggcga cgtgtacatc tgcctgacca gattcatcgg cgacagcaaa gtgatgaagt
     5041 ggggcggcag cagctttcag gacatccaga ggatgcctct caggggcagc atggtgtttc
     5101 agcccctgca gatcaacaac taccagtacg ccatcctggg cagcgactac agcttcaccc
     5161 aggtgtacaa ttgggacgcc gagaaggcca agttcgtgaa gttccaagag ctgaacgtgc
     5221 aggcccctag atctttcacc cacgtgtcaa tcaacaagcg gaacttcctg ttcgccagca
     5281 gcttcaaggg caacacccag atctacaagc acgtcatcgt ggatctgagc gccagcggct
     5341 ctactagtgg tggatctggt ggaaccggcg gctccaaagg cgaggaactg tttacaggcg
     5401 tggtgcctat cctggtcgag ctggatgggg atgtgaacgg ccacaagttc tccgtgtctg
     5461 gcgaaggcga aggggacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg
     5521 gcaagctgcc tgtgccttgg cctacactgg tcaccacact gacatacggc gtgcagtgct
     5581 tcagcagata ccccgaccat atgaagaggc acgacttctt caagagcgcc atgcctgagg
     5641 gctacgtgca agagaggacc atcttcttta aggacgacgg caactacaag accagggccg
     5701 aagtgaagtt cgagggcgac accctggtca acaggatcga gctgaaggga atcgacttca
     5761 aagaggacgg caacatcctg ggacacaagt tggagtacaa ctacaacgat caccaggtct
     5821 acatcatggc cgataagcag aagaacggca tcaaggccaa cttcaagatc cggcacaaca
     5881 tcgaggacgg cggagtgcag ctggccgatc actatcagca gaacacccct atcggcgacg
     5941 gacctgtgct gctgcctgat aaccactacc tgtttaccac cagcacactg agcaaggacc
     6001 ccaacgagaa gagggaccac atggtgctgt tggagttcgt gacagccgcc ggaatcacac
     6061 acggaatgga cgagctgtac aaatgagaat tcgatatcaa gcttatcgat aatcaacctc
     6121 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6181 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6241 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6301 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6361 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6421 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6481 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6541 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6601 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6661 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     6721 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6781 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6901 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6961 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     7021 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7081 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     7141 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     7201 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7381 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     7441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7561 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7621 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7681 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7861 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7921 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8041 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8101 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8161 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8221 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8281 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8401 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8461 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     8521 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     8581 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     8641 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     8701 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     8761 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     8821 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     8881 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     8941 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     9001 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     9061 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     9121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9241 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9421 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10081 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    10141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10741 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11041 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11401 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    11461 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    11521 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    11581 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    11641 gccagatata cgcgtt
//
