LOCUS       sequence-369449-       11656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-Y433A-pHluorin
FEATURES             Location/Qualifiers
     source          1..11656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2372..2570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2588..2768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2815..2940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3433..3666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3851..3895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4193..4310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4355..5643
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(7663..7682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    8101..8689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8154..8174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8572..8583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8692..8708)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8693..8709)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9214..9394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9420..9437)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9426..9650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9696..10124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9783..9802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9993..10014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10133..10153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10138..10467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10318..10453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10380..10399
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10515..10562
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10581..10955
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11085..11206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11122..11141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11176..11195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11255..11271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11255..11271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11268..11290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11279..11295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11303..11309,11310..11327,11328..11333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11348..11369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11486..11503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatggaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2581 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2641 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2701 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2761 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2821 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2881 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2941 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3001 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3061 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3121 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3181 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3241 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3301 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3361 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3421 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3481 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3541 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3601 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3661 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3721 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3781 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3841 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3901 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3961 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4021 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4081 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4141 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4201 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4261 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4321 acagggacag cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct
     4381 ccatggggtt cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca
     4441 gtgacctgcc cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa
     4501 tcttaaattc catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta
     4561 gggcagatga ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc
     4621 cgcccagccc tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc
     4681 taccttgacg ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca
     4741 tggtccctct gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc
     4801 tgctcagtgg tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg
     4861 gtctgtttgg tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa
     4921 gcagggagag ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc
     4981 ccagaggaag caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat
     5041 tagcttcccc tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc
     5101 tcttcagagg agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc
     5161 agaatgtggc acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca
     5221 tcccgtctgt taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca
     5281 cttgtggact aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg
     5341 gaacagggtc cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca
     5401 gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg
     5461 gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag
     5521 gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa
     5581 gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc
     5641 agcgggggat ccatggaaag cgagagcatc agaaggatgg gcaacgcttg catccctctg
     5701 aagaggatcg cctacttcct gtgcctgttc agcgtggtgc tgctgaccga gggaaagaag
     5761 cctgccaagc ctaagtgccc tgccgtgtgc acctgtagca aggataacgc cctgtgcgag
     5821 aacgcccgca gcatccctag aacagtgcct ccagacgtga tcagcctgag ctttgtgcgg
     5881 agcggcttca ccgagatcag cgagggatct ttcctgttca cccctagcct gcagctgctg
     5941 ctgttcacca gcaacagctt cgacgtgatc tccgacgacg ccttcatcgg cctgcctcac
     6001 ctggaatacc tgttcatcga gaacaacaac atcaagagca tctccaggca caccttcagg
     6061 ggcctgaagt ccctgatcca cctgagcctg gccaacaaca acctgcagac cctgcctaag
     6121 gacatcttca aaggcctgga cagcctgacc aacgtggacc tgagaggcaa ctccttcaac
     6181 tgcgactgca agctgaagtg gctggtggaa tggctgggcc acacaaacgc taccgtcgag
     6241 gacatctact gcgagggacc tccagagtac aagaagcgga agatcaactc tctgagcccc
     6301 aaggacttcg actgcatcat caccgagttc gccaagagcc aggacctgcc ttaccagagc
     6361 ctgagcatcg acaccttctc ttacctgaac gacgagtacg tcgtgatcgc ccagcctttt
     6421 accggcaagt gcatcttcct ggaatgggac cacgtggaaa agaccttccg gaactacgac
     6481 aacatcaccg gcaccagcac cgtcgtgtgc aagcctatcg tgatcgacac ccagctgtac
     6541 gtgatcgtgg ctcagctgtt cggcggcagc cacatctaca agagagatgg cttcgccaac
     6601 aagttcatca agatccagga catcgaggtg ctgaagatca gaaagcccaa cgacatcgag
     6661 acattcaaga tcgaggataa ctggtacttc gtggtggccg acagcagcaa ggccggcttc
     6721 acaacaatct acaagtggaa cggcaacggc ttctacagcc accagtctct gcacgcctgg
     6781 tacagagaca ccgacgtcga gtacctggaa atcgccaggc ctcctctgac actgagaacc
     6841 cctcacctga tcctgagcag ctctagccag aggcctgtga tctaccagtg gtccaaggcc
     6901 acacagctgt tcatcaacca gacagacatc cccaacatgg aagatgtggc cgccgtgaag
     6961 cacttcagcg tgaagggcga cgtgtacatc tgcctgacca gattcatcgg cgacagcaaa
     7021 gtgatgaagt ggggcggcag cagctttcag gacatccaga ggatgcctag caggggcagc
     7081 atggtgtttc agcccctgca gatcaacaac taccagtacg ccatcctggg cagcgactac
     7141 agcttcaccc aggtgtacaa ttgggacgcc gagaaggcca agttcgtgaa gttccaagag
     7201 ctgaacgtgc aggcccctag atctttcacc cacgtgtcaa tcaacaagcg gaacttcctg
     7261 ttcgccagca gcttcaaggg caacacccag atctacaagc acgtcatcgt ggatctgagc
     7321 gccagcggct ctactagtgg tggatctggt ggaaccggcg gctccaaagg cgaggaactg
     7381 tttacaggcg tggtgcctat cctggtcgag ctggatgggg atgtgaacgg ccacaagttc
     7441 tccgtgtctg gcgaaggcga aggggacgcc acatacggaa agctgaccct gaagttcatc
     7501 tgcaccaccg gcaagctgcc tgtgccttgg cctacactgg tcaccacact gacatacggc
     7561 gtgcagtgct tcagcagata ccccgaccat atgaagaggc acgacttctt caagagcgcc
     7621 atgcctgagg gctacgtgca agagaggacc atcttcttta aggacgacgg caactacaag
     7681 accagggccg aagtgaagtt cgagggcgac accctggtca acaggatcga gctgaaggga
     7741 atcgacttca aagaggacgg caacatcctg ggacacaagt tggagtacaa ctacaacgat
     7801 caccaggtct acatcatggc cgataagcag aagaacggca tcaaggccaa cttcaagatc
     7861 cggcacaaca tcgaggacgg cggagtgcag ctggccgatc actatcagca gaacacccct
     7921 atcggcgacg gacctgtgct gctgcctgat aaccactacc tgtttaccac cagcacactg
     7981 agcaaggacc ccaacgagaa gagggaccac atggtgctgt tggagttcgt gacagccgcc
     8041 ggaatcacac acggaatgga cgagctgtac aaatgagaat tcgatatcaa gcttatcgat
     8101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8221 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8281 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8341 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8401 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8461 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8521 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8581 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8641 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     8701 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     8761 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     8821 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8881 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     8941 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     9001 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     9061 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     9121 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     9181 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     9241 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9301 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9361 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     9421 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9481 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9541 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9601 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9661 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     9721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
    10141 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10201 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    10261 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    10321 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    10381 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
    10441 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
    10501 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
    10561 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
    10621 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
    10681 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
    10741 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
    10801 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
    10861 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
    10921 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
    10981 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    11041 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    11101 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    11161 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    11221 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    11281 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    11341 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    11401 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11521 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11581 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11641 ccgcgttgct ggcgtt
//
