LOCUS       sequence-369450-       11656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-R474Q-pHluorin
FEATURES             Location/Qualifiers
     source          1..11656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1041..1060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1232..1611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1613..1811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1766..1786
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1829..2009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2056..2181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2674..2907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3092..3136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3434..3551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3596..4884
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(6904..6923)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    7342..7930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7395..7415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7813..7824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7933..7949)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7934..7950)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8455..8635
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8661..8678)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8667..8891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8937..9365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9024..9043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9234..9255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9374..9394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9379..9708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9559..9694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9621..9640
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9756..9803
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9822..10196
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10326..10447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10363..10382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10417..10436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10496..10512)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10496..10512)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10509..10531)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10520..10536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10544..10550,10551..10568,10569..10574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10589..10610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10727..10744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10898..11486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10978..10997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     1021 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     1081 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     1141 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     1201 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     1261 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1321 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1381 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1441 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1501 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1561 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1621 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1681 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1741 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     1801 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     1861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1981 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     2041 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     2101 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     2161 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     2221 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     2281 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     2341 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     2401 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     2461 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     2521 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2581 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2641 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2701 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2761 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2821 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2881 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2941 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     3001 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     3061 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     3121 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     3181 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     3241 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     3301 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     3361 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     3421 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     3481 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3541 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaacatta
     3601 tggccttagg tcacttcatc tccatggggt tcttcttctg attttctaga aaatgagatg
     3661 ggggtgcaga gagcttcctc agtgacctgc ccagggtcac atcagaaatg tcagagctag
     3721 aacttgaact cagattacta atcttaaatt ccatgccttg ggggcatgca agtacgatat
     3781 acagaaggag tgaactcatt agggcagatg accaatgagt ttaggaaaga agagtccagg
     3841 gcagggtaca tctacaccac ccgcccagcc ctgggtgagt ccagccacgt tcacctcatt
     3901 atagttgcct ctctccagtc ctaccttgac gggaagcaca agcagaaact gggacaggag
     3961 ccccaggaga ccaaatcttc atggtccctc tgggaggatg ggtggggaga gctgtggcag
     4021 aggcctcagg aggggccctg ctgctcagtg gtgacagata ggggtgagaa agcagacaga
     4081 gtcattccgt cagcattctg ggtctgtttg gtacttcttc tcacgctaag gtggcggtgt
     4141 gatatgcaca atggctaaaa agcagggaga gctggaaaga aacaaggaca gagacagagg
     4201 ccaagtcaac cagaccaatt cccagaggaa gcaaagaaac cattacagag actacaaggg
     4261 ggaagggaag gagagatgaa ttagcttccc ctgtaaacct tagaacccag ctgttgccag
     4321 ggcaacgggg caatacctgt ctcttcagag gagatgaagt tgccagggta actacatcct
     4381 gtctttctca aggaccatcc cagaatgtgg cacccactag ccgttaccat agcaactgcc
     4441 tctttgcccc acttaatccc atcccgtctg ttaaaagggc cctatagttg gaggtggggg
     4501 aggtaggaag agcgatgatc acttgtggac taagtttgtt cgcatcccct tctccaaccc
     4561 cctcagtaca tcaccctggg ggaacagggt ccacttgctc ctgggcccac acagtcctgc
     4621 agtattgtgt atataaggcc agggcaaaga ggagcaggtt ttaaagtgaa aggcaggcag
     4681 gtgttgggga ggcagttacc ggggcaacgg gaacagggcg tttcggaggt ggttgccatg
     4741 gggacctgga tgctgacgaa ggctcgcgag gctgtgagca gccacagtgc cctgctcaga
     4801 agccccaagc tcgtcagtca agccggttct ccgtttgcac tcaggagcac gggcaggcga
     4861 gtggccccta gttctggggg cagcggggga tccatggaaa gcgagagcat cagaaggatg
     4921 ggcaacgctt gcatccctct gaagaggatc gcctacttcc tgtgcctgtt cagcgtggtg
     4981 ctgctgaccg agggaaagaa gcctgccaag cctaagtgcc ctgccgtgtg cacctgtagc
     5041 aaggataacg ccctgtgcga gaacgcccgc agcatcccta gaacagtgcc tccagacgtg
     5101 atcagcctga gctttgtgcg gagcggcttc accgagatca gcgagggatc tttcctgttc
     5161 acccctagcc tgcagctgct gctgttcacc agcaacagct tcgacgtgat ctccgacgac
     5221 gccttcatcg gcctgcctca cctggaatac ctgttcatcg agaacaacaa catcaagagc
     5281 atctccaggc acaccttcag gggcctgaag tccctgatcc acctgagcct ggccaacaac
     5341 aacctgcaga ccctgcctaa ggacatcttc aaaggcctgg acagcctgac caacgtggac
     5401 ctgagaggca actccttcaa ctgcgactgc aagctgaagt ggctggtgga atggctgggc
     5461 cacacaaacg ctaccgtcga ggacatctac tgcgagggac ctccagagta caagaagcgg
     5521 aagatcaact ctctgagccc caaggacttc gactgcatca tcaccgagtt cgccaagagc
     5581 caggacctgc cttaccagag cctgagcatc gacaccttct cttacctgaa cgacgagtac
     5641 gtcgtgatcg cccagccttt taccggcaag tgcatcttcc tggaatggga ccacgtggaa
     5701 aagaccttcc ggaactacga caacatcacc ggcaccagca ccgtcgtgtg caagcctatc
     5761 gtgatcgaca cccagctgta cgtgatcgtg gctcagctgt tcggcggcag ccacatctac
     5821 aagagagatg gcttcgccaa caagttcatc aagatccagg acatcgaggt gctgaagatc
     5881 agaaagccca acgacatcga gacattcaag atcgaggata actggtactt cgtggtggcc
     5941 gacagcagca aggccggctt cacaacaatc tacaagtgga acggcaacgg cttctacagc
     6001 caccagtctc tgcacgcctg gtacagagac accgacgtcg agtacctgga aatcgccagg
     6061 cctcctctga cactgagaac ccctcacctg atcctgagca gctctagcca gaggcctgtg
     6121 atctaccagt ggtccaaggc cacacagctg ttcatcaacc agacagacat ccccaacatg
     6181 gaagatgtgt acgccgtgaa gcacttcagc gtgaagggcg acgtgtacat ctgcctgacc
     6241 agattcatcg gcgacagcaa agtgatgaag tggggcggca gcagctttca ggacatccag
     6301 aggatgccta gccagggcag catggtgttt cagcccctgc agatcaacaa ctaccagtac
     6361 gccatcctgg gcagcgacta cagcttcacc caggtgtaca attgggacgc cgagaaggcc
     6421 aagttcgtga agttccaaga gctgaacgtg caggccccta gatctttcac ccacgtgtca
     6481 atcaacaagc ggaacttcct gttcgccagc agcttcaagg gcaacaccca gatctacaag
     6541 cacgtcatcg tggatctgag cgccagcggc tctactagtg gtggatctgg tggaaccggc
     6601 ggctccaaag gcgaggaact gtttacaggc gtggtgccta tcctggtcga gctggatggg
     6661 gatgtgaacg gccacaagtt ctccgtgtct ggcgaaggcg aaggggacgc cacatacgga
     6721 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccttg gcctacactg
     6781 gtcaccacac tgacatacgg cgtgcagtgc ttcagcagat accccgacca tatgaagagg
     6841 cacgacttct tcaagagcgc catgcctgag ggctacgtgc aagagaggac catcttcttt
     6901 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctggtc
     6961 aacaggatcg agctgaaggg aatcgacttc aaagaggacg gcaacatcct gggacacaag
     7021 ttggagtaca actacaacga tcaccaggtc tacatcatgg ccgataagca gaagaacggc
     7081 atcaaggcca acttcaagat ccggcacaac atcgaggacg gcggagtgca gctggccgat
     7141 cactatcagc agaacacccc tatcggcgac ggacctgtgc tgctgcctga taaccactac
     7201 ctgtttacca ccagcacact gagcaaggac cccaacgaga agagggacca catggtgctg
     7261 ttggagttcg tgacagccgc cggaatcaca cacggaatgg acgagctgta caaatgagaa
     7321 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     7381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7741 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7861 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7921 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     7981 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8041 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8101 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8161 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     8221 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8281 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8341 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8401 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8461 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8521 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8581 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     8641 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8701 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8761 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8821 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8881 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8941 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9001 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9061 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9121 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9181 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9241 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9301 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9361 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9481 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     9661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     9721 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     9781 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     9841 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     9901 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     9961 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    10021 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    10081 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    10141 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
    10201 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10321 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10441 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10501 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10561 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10621 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10681 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11641 aaacttggtc tgacag
//
