LOCUS       sequence-369452-       11668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant E383A LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-E383A-pHluorin
FEATURES             Location/Qualifiers
     source          1..11668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3900..3909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5916..5935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6360..6948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6413..6433)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6831..6842)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6951..6967)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6952..6968)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7473..7653
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7679..7696)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7685..7909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7955..8383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8042..8061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8252..8273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8392..8412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8397..8726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8577..8712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8639..8658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8774..8821
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8840..9214
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9344..9465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9381..9400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9435..9454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9514..9530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9514..9530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9527..9549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9538..9554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9562..9568,9569..9586,9587..9592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9607..9628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9745..9762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9916..10504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9996..10015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10675..11466,11467..11535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11298..11317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11536..11640)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctmgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
     3901 ccaccatgga aagcgagagc atcagaagga tgggcaacgc ttgcatccct ctgaagagga
     3961 tcgcctactt cctgtgcctg ttcagcgtgg tgctgctgac cgagggaaag aagcctgcca
     4021 agcctaagtg ccctgccgtg tgcacctgta gcaaggataa cgccctgtgc gagaacgccc
     4081 gcagcatccc tagaacagtg cctccagacg tgatcagcct gagctttgtg cggagcggct
     4141 tcaccgagat cagcgaggga tctttcctgt tcacccctag cctgcagctg ctgctgttca
     4201 ccagcaacag cttcgacgtg atctccgacg acgccttcat cggcctgcct cacctggaat
     4261 acctgttcat cgagaacaac aacatcaaga gcatctccag gcacaccttc aggggcctga
     4321 agtccctgat ccacctgagc ctggccaaca acaacctgca gaccctgcct aaggacatct
     4381 tcaaaggcct ggacagcctg accaacgtgg acctgagagg caactccttc aactgcgact
     4441 gcaagctgaa gtggctggtg gaatggctgg gccacacaaa cgctaccgtc gaggacatct
     4501 actgcgaggg acctccagag tacaagaagc ggaagatcaa ctctctgagc cccaaggact
     4561 tcgactgcat catcaccgag ttcgccaaga gccaggacct gccttaccag agcctgagca
     4621 tcgacacctt ctcttacctg aacgacgagt acgtcgtgat cgcccagcct tttaccggca
     4681 agtgcatctt cctggaatgg gaccacgtgg aaaagacctt ccggaactac gacaacatca
     4741 ccggcaccag caccgtcgtg tgcaagccta tcgtgatcga cacccagctg tacgtgatcg
     4801 tggctcagct gttcggcggc agccacatct acaagagaga tggcttcgcc aacaagttca
     4861 tcaagatcca ggacatcgag gtgctgaaga tcagaaagcc caacgacatc gagacattca
     4921 agatcgagga taactggtac ttcgtggtgg ccgacagcag caaggccggc ttcacaacaa
     4981 tctacaagtg gaacggcaac ggcttctaca gccaccagtc tctgcacgcc tggtacagag
     5041 acaccgacgt cgcgtacctg gaaatcgcca ggcctcctct gacactgaga acccctcacc
     5101 tgatcctgag cagctctagc cagaggcctg tgatctacca gtggtccaag gccacacagc
     5161 tgttcatcaa ccagacagac atccccaaca tggaagatgt gtacgccgtg aagcacttca
     5221 gcgtgaaggg cgacgtgtac atctgcctga ccagattcat cggcgacagc aaagtgatga
     5281 agtggggcgg cagcagcttt caggacatcc agaggatgcc tagcaggggc agcatggtgt
     5341 ttcagcccct gcagatcaac aactaccagt acgccatcct gggcagcgac tacagcttca
     5401 cccaggtgta caattgggac gccgagaagg ccaagttcgt gaagttccaa gagctgaacg
     5461 tgcaggcccc tagatctttc acccacgtgt caatcaacaa gcggaacttc ctgttcgcca
     5521 gcagcttcaa gggcaacacc cagatctaca agcacgtcat cgtggatctg agcgccagcg
     5581 gctctactag tggtggatct ggtggaaccg gcggctccaa aggcgaggaa ctgtttacag
     5641 gcgtggtgcc tatcctggtc gagctggatg gggatgtgaa cggccacaag ttctccgtgt
     5701 ctggcgaagg cgaaggggac gccacatacg gaaagctgac cctgaagttc atctgcacca
     5761 ccggcaagct gcctgtgcct tggcctacac tggtcaccac actgacatac ggcgtgcagt
     5821 gcttcagcag ataccccgac catatgaaga ggcacgactt cttcaagagc gccatgcctg
     5881 agggctacgt gcaagagagg accatcttct ttaaggacga cggcaactac aagaccaggg
     5941 ccgaagtgaa gttcgagggc gacaccctgg tcaacaggat cgagctgaag ggaatcgact
     6001 tcaaagagga cggcaacatc ctgggacaca agttggagta caactacaac gatcaccagg
     6061 tctacatcat ggccgataag cagaagaacg gcatcaaggc caacttcaag atccggcaca
     6121 acatcgagga cggcggagtg cagctggccg atcactatca gcagaacacc cctatcggcg
     6181 acggacctgt gctgctgcct gataaccact acctgtttac caccagcaca ctgagcaagg
     6241 accccaacga gaagagggac cacatggtgc tgttggagtt cgtgacagcc gccggaatca
     6301 cacacggaat ggacgagctg tacaaatgag atatcgaatt cgatatcaag cttatcgata
     6361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6481 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6541 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6601 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6661 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6721 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6781 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6841 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6901 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     6961 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7021 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     7081 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7141 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     7201 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     7261 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     7321 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     7381 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     7441 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     7501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7621 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     7681 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7741 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7801 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7861 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7921 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     7981 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8041 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8101 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8161 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8221 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8281 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8341 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     8401 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8461 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8521 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8641 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     8701 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     8761 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     8821 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     8881 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     8941 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     9001 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     9061 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     9121 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     9181 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     9241 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     9301 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     9361 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9421 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     9481 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9541 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9601 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9661 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9721 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9781 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9841 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9901 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9961 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10021 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10081 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10141 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10201 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10261 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10321 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    10381 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10441 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10501 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10561 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10621 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10681 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10741 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10801 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10861 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10921 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10981 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11041 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11101 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11161 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11221 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11281 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11341 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11401 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11461 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11521 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11581 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11641 cacatttccc cgaaaagtgc cacctgac
//
