LOCUS       sequence-369453-       11656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant C200R LGI1-pHluorin under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost 
            A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, 
            Hoppa MB, de Juan-Sanz J
  TITLE     Activity-driven synaptic translocation of LGI1 controls excitatory 
            neurotransmission.
  JOURNAL   Cell Rep. 2024 May 28;43(5):114186. doi: 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2.
  PUBMED    38700985
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-05-28"; volume: "43(5):114186. doi"; pages: " 
            10.1016/j.celrep.2024.114186. Epub 2024 May 2"
COMMENT     Sequence Label: CamKII-LGI1-C200R-pHluorin
FEATURES             Location/Qualifiers
     source          1..11656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..76)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        248..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     782..802
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             845..1025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1072..1197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1690..1923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2108..2152
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2612..3900
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(5920..5939)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6358..6946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6411..6431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6829..6840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6949..6965)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6950..6966)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7471..7651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7677..7694)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7683..7907
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7953..8381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8040..8059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8250..8271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8390..8410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8395..8724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8575..8710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8637..8656
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8772..8819
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8838..9212
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9342..9463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9379..9398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9433..9452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9512..9528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9512..9528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9525..9547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9536..9552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9560..9566,9567..9584,9585..9590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9605..9626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9743..9760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9914..10502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9994..10013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10673..11464,11465..11533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11296..11315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11534..11638)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
       61 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
      121 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
      181 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      241 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      301 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      361 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      421 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      481 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      541 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      601 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      661 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      721 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      781 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      841 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1021 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     1081 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1141 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1201 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1261 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1321 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1381 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1441 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1501 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1561 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1621 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1681 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1741 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1801 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1861 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1921 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1981 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     2041 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     2101 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2161 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2221 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2281 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2341 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2401 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca
     2641 tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg
     2701 acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct
     2761 taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg
     2821 cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc
     2881 ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac
     2941 cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg
     3001 tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc
     3061 tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc
     3121 tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca
     3181 gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca
     3241 gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag
     3301 cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct
     3361 tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga
     3421 atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc
     3481 cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt
     3541 gtggactaag tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa
     3601 cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg
     3661 caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg
     3721 caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct
     3781 cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc
     3841 ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc
     3901 gggggatcca tggaaagcga gagcatcaga aggatgggca acgcttgcat ccctctgaag
     3961 aggatcgcct acttcctgtg cctgttcagc gtggtgctgc tgaccgaggg aaagaagcct
     4021 gccaagccta agtgccctgc cgtgtgcacc tgtagcaagg ataacgccct gtgcgagaac
     4081 gcccgcagca tccctagaac agtgcctcca gacgtgatca gcctgagctt tgtgcggagc
     4141 ggcttcaccg agatcagcga gggatctttc ctgttcaccc ctagcctgca gctgctgctg
     4201 ttcaccagca acagcttcga cgtgatctcc gacgacgcct tcatcggcct gcctcacctg
     4261 gaatacctgt tcatcgagaa caacaacatc aagagcatct ccaggcacac cttcaggggc
     4321 ctgaagtccc tgatccacct gagcctggcc aacaacaacc tgcagaccct gcctaaggac
     4381 atcttcaaag gcctggacag cctgaccaac gtggacctga gaggcaactc cttcaactgc
     4441 gactgcaagc tgaagtggct ggtggaatgg ctgggccaca caaacgctac cgtcgaggac
     4501 atctaccgcg agggacctcc agagtacaag aagcggaaga tcaactctct gagccccaag
     4561 gacttcgact gcatcatcac cgagttcgcc aagagccagg acctgcctta ccagagcctg
     4621 agcatcgaca ccttctctta cctgaacgac gagtacgtcg tgatcgccca gccttttacc
     4681 ggcaagtgca tcttcctgga atgggaccac gtggaaaaga ccttccggaa ctacgacaac
     4741 atcaccggca ccagcaccgt cgtgtgcaag cctatcgtga tcgacaccca gctgtacgtg
     4801 atcgtggctc agctgttcgg cggcagccac atctacaaga gagatggctt cgccaacaag
     4861 ttcatcaaga tccaggacat cgaggtgctg aagatcagaa agcccaacga catcgagaca
     4921 ttcaagatcg aggataactg gtacttcgtg gtggccgaca gcagcaaggc cggcttcaca
     4981 acaatctaca agtggaacgg caacggcttc tacagccacc agtctctgca cgcctggtac
     5041 agagacaccg acgtcgagta cctggaaatc gccaggcctc ctctgacact gagaacccct
     5101 cacctgatcc tgagcagctc tagccagagg cctgtgatct accagtggtc caaggccaca
     5161 cagctgttca tcaaccagac agacatcccc aacatggaag atgtgtacgc cgtgaagcac
     5221 ttcagcgtga agggcgacgt gtacatctgc ctgaccagat tcatcggcga cagcaaagtg
     5281 atgaagtggg gcggcagcag ctttcaggac atccagagga tgcctagcag gggcagcatg
     5341 gtgtttcagc ccctgcagat caacaactac cagtacgcca tcctgggcag cgactacagc
     5401 ttcacccagg tgtacaattg ggacgccgag aaggccaagt tcgtgaagtt ccaagagctg
     5461 aacgtgcagg cccctagatc tttcacccac gtgtcaatca acaagcggaa cttcctgttc
     5521 gccagcagct tcaagggcaa cacccagatc tacaagcacg tcatcgtgga tctgagcgcc
     5581 agcggctcta ctagtggtgg atctggtgga accggcggct ccaaaggcga ggaactgttt
     5641 acaggcgtgg tgcctatcct ggtcgagctg gatggggatg tgaacggcca caagttctcc
     5701 gtgtctggcg aaggcgaagg ggacgccaca tacggaaagc tgaccctgaa gttcatctgc
     5761 accaccggca agctgcctgt gccttggcct acactggtca ccacactgac atacggcgtg
     5821 cagtgcttca gcagataccc cgaccatatg aagaggcacg acttcttcaa gagcgccatg
     5881 cctgagggct acgtgcaaga gaggaccatc ttctttaagg acgacggcaa ctacaagacc
     5941 agggccgaag tgaagttcga gggcgacacc ctggtcaaca ggatcgagct gaagggaatc
     6001 gacttcaaag aggacggcaa catcctggga cacaagttgg agtacaacta caacgatcac
     6061 caggtctaca tcatggccga taagcagaag aacggcatca aggccaactt caagatccgg
     6121 cacaacatcg aggacggcgg agtgcagctg gccgatcact atcagcagaa cacccctatc
     6181 ggcgacggac ctgtgctgct gcctgataac cactacctgt ttaccaccag cacactgagc
     6241 aaggacccca acgagaagag ggaccacatg gtgctgttgg agttcgtgac agccgccgga
     6301 atcacacacg gaatggacga gctgtacaaa tgagaattcg atatcaagct tatcgataat
     6361 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6421 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6481 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6541 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6601 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6721 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6781 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6841 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6901 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6961 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     7021 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     7081 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7141 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     7201 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     7261 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     7321 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     7381 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     7441 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     7501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7621 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     7681 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7741 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7801 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7861 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7921 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8161 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8281 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8401 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8461 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8521 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8641 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     8701 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     8761 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     8821 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     8881 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     8941 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     9001 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     9061 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     9121 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     9181 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     9241 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     9301 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     9361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9481 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11641 catttccccg aaaagt
//
