LOCUS       sequence-369461-       18130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of HUWE1-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_HUWE1_3xFLAG
FEATURES             Location/Qualifiers
     source          1..18130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             16355..16378
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             16397..16462
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(16557..16577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16975..16986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17053..17070)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17059..17266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(17314..17319,17320..17336,17337..17342)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17531..18064
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccgtcgac accatgaaag tagacaggac
     3241 taaactgaag aagacaccta ctgaggctcc tgcagactgc agagccttaa tagacaaact
     3301 caaagtttgt aatgatgagc aacttctctt ggaactgcag cagatcaaaa catggaacat
     3361 tggaaagtgc gagttatatc actgggtgga cctgttggac cgcttcgatg gaatactggc
     3421 agatgctgga cagacagtgg agaatatgtc atggatgctc gtatgtgata ggccagaaag
     3481 agagcaactg aaaatgcttc tcttggctgt gttgaacttc acagccttgc tcattgagta
     3541 cagcttttcc cggcatctgt acagttccat agagcatttg acaactttat tggcttcctc
     3601 tgatatgcaa gtggtgctgg cagtcctcaa tctcctatat gtatttagca aaagatcaaa
     3661 ctacatcact cgtctgggat ctgacaagag gaccccgctg ctaactcggc tacaacattt
     3721 ggcagagagc tggggtggaa aggagaatgg ctttggactt gcagaatgtt gcagagactt
     3781 gcatatgatg aaatatccac ccagtgcaac tacactacac tttgaattct atgcagatcc
     3841 tggggccgag gtcaaaattg agaaaaggac aactagtaac acactacatt atattcacat
     3901 agagcaactt gacaagattt cagaaagccc ttctgaaatc atggaatctc ttaccaaaat
     3961 gtacagcatt cctaaggata agcagatgct gttatttaca cacatacgac tggcccatgg
     4021 cttttctaat cacaggaagc gattgcaggc agttcaggcc agactgcatg caatatctat
     4081 attagtgtat tccaatgcct tgcaggaatc agcaaacagt atcttgtata atggcttgat
     4141 agaggagtta gtggacgtgc tgcaaataac ggataagcag cttatggaga ttaaagcagc
     4201 ttctttacga acattaacat caattgtcca cttggagaga actcccaaac tcagcagtat
     4261 tattgactgt actggaactg cctcctacca tggatttttg ccagtgcttg taaggaactg
     4321 tatccaggcc atgattgatc cttccatgga tccataccct caccagtttg ccactgctct
     4381 cttctctttt ttataccatc tggccagcta cgatgctggt ggtgaagcct tggtctcctg
     4441 tggaatgatg gaagccttat tgaaggtcat aaagtttctt ggcgatgaac aggaccagat
     4501 aacatttgtc accagagccg tcagagtggt tgaccttatc accaacctgg atatggcagc
     4561 ttttcaatcc catagtggac tttctatctt catttataga cttgagcatg aagtagatct
     4621 gtgccgaaaa gaatgtccgt ttgtgatcaa gccaaagatc cagagaccca atactacaca
     4681 agaaggagag gaaatggaaa ctgatatgga tggagtccag tgtattccac aacgagcagc
     4741 acttctgaaa tccatgttga atttcctcaa gaaggccatc caagaccctg ctttctcaga
     4801 tggcatacga catgtgatgg atggttctct gcctacctcc ctgaaacaca tcatcagcaa
     4861 tgcagaatac tatggcccat cactcttcct cctagctact gaagtggtga ctgtgtttgt
     4921 atttcaagaa ccatcactgc tctcctcact ccaggacaat ggattgacag atgtcatgct
     4981 gcatgcactg cttatcaaag atgttcctgc tacccgtgaa gtccttggct ccctcccaaa
     5041 tgtattcagt gcactctgtt tgaatgcccg aggtcttcag tcttttgttc agtgtcagcc
     5101 ttttgaacgc ctcttcaaag ttcttctgtc tccagattac ctcccagcca tgcggaggag
     5161 gagaagttct gatccccttg gggatactgc atccaacctg gggagtgctg tcgatgagct
     5221 catgagacat cagcccaccc ttaaaacaga tgcaacgact gccatcatca agttacttga
     5281 agaaatctgt aatcttggaa gggaccccaa atacatctgt cagaagccat cgatccagaa
     5341 ggcagatggc actgccactg ctcctccccc aaggtctaat catgccgcag aagaagcctc
     5401 tagtgaggat gaggaggaag aggaagtaca ggccatgcag agctttaatt ctacccagca
     5461 aaatgaaact gagcctaatc agcaggttgt tggtacagag gaacgtattc ctattcccct
     5521 catggattac atccttaatg tgatgaaatt tgtggaatct attctgagca acaatacaac
     5581 agatgaccac tgccaggaat ttgtgaatca gaaaggactg ttgcctttgg ttaccatttt
     5641 gggtcttccc aatctgccca ttgactttcc cacatctgct gcctgtcagg ctgttgcagg
     5701 tgtctgcaaa tccatattga cactgtcaca tgaacccaaa gtccttcaag agggtctcct
     5761 tcagttggac tccatcctct cctccctgga gcccttacac cgccccattg aatcccctgg
     5821 gggctcagtg ttgttgcgag aactggcttg cgcaggcaat gttgctgatg ctaccctctc
     5881 agcccaggcc acacctctgc tgcatgcact cactgctgcc catgcctaca tcatgatgtt
     5941 tgttcatact tgcagagttg gacagagtga aattcgttcc atctccgtaa accagtgggg
     6001 ctctcaattg ggtctgagtg ttttgagcaa gctgagccag ttatactgtt ccctggtgtg
     6061 ggaaagcact gtcctcctct ctctgtgtac cccaaacagc ctaccatctg ggtgtgaatt
     6121 tggccaggca gatatgcaga aactggttcc aaaggatgag aaggcaggta cgacccaggg
     6181 cggaaaaaga tcagatgggg aacaggatgg agcagctgga agtatggatg cttctaccca
     6241 gggcttatta gaaggcattg ggctagatgg tgacacattg gctcccatgg agacagatga
     6301 acctactgct tcagactcta agggcaaatc taaaatcaca ccagcaatgg ctgccagaat
     6361 taagcaaatc aagcctttgt tatcagcttc ctccagatta ggccgagcac ttgctgagct
     6421 atttggactt cttgttaaac tttgtgtggg atctcctgtc cgccagagaa ggagccatca
     6481 tgctgccagc accactacag caccgacacc tgccgcgcga tcaacagcct cagctctcac
     6541 taagctcttg actaaggggt tatcttggca gcccccacca tatacaccta ctccccgatt
     6601 caggctgaca ttcttcatct gttcagttgg tttcacatcc ccaatgctgt ttgatgagag
     6661 gaagtatccc taccacctca tgctgcaaaa atttctctgc tccggaggcc acaatgctct
     6721 ttttgaaact ttcaactggg ctctgtccat gggaggtaaa gttcctgttt ctgagggatt
     6781 ggaacactca gacttgcctg atggcacagg agaattccta gatgcctggc ttatgctggt
     6841 ggagaagatg gtgaatccca ccacggtgct tgaatctcca cattcgctgc ctgccaaatt
     6901 gcctggaggt gtccagaact ttccccagtt cagtgcactg cgcttccttg tggtaactca
     6961 gaaagcagcc tttacttgca tcaaaaactt atggaaccgg aaacccctga aggtatatgg
     7021 tggacgaatg gctgaatcga tgctggccat tctatgccac atcctccgag gagaacctgt
     7081 gattcgagag agactaagca aggagaagga ggggtctcga ggagaagagg atacagggca
     7141 agaggaaggt ggctcccgcc gggaacctca agtcaaccag caacaactgc aacagctcat
     7201 ggacatgggc ttcacaaggg aacatgcaat ggaggcactg ttgaacacca gcaccatgga
     7261 gcaggccaca gagtaccttt taacccaccc tcctccaatc atgggaggag ttgttcggga
     7321 tctcagcatg tctgaagagg accagatgat gagagcaatt gctatgtctc tgggacagga
     7381 tattccaatg gatcaaaggg cagagtcacc tgaggaagtt gcttgccgga aggaggaaga
     7441 ggaacggaaa gctcgggaaa agcaggagga ggaagaggct aaatgtctag agaagttcca
     7501 ggatgctgac ccgttggaac aagatgagct ccacactttc acagatacta tgttgccagg
     7561 ctgcttccac cttcttgatg agctgccaga cacagtatac cgtgtgtgtg acctgatcat
     7621 gacagcaatc aaacgtaatg gagcagatta tcgtgacatg attctgaagc aagtagtcaa
     7681 tcaggtgtgg gaagctgctg atgtattgat caaagctgct cttcccctga caacaagtga
     7741 cacaaaaacc gtgtcagagt ggataagtca gatggccaca ctgccccagg cctccaattt
     7801 ggctactaga atcttgcttt taacgctact ttttgaggag ttgaagctac cttgtgcttg
     7861 ggtggttgaa tcaagtggca tccttaatgt cctaatcaaa ctcttggaag tggttcagcc
     7921 ctgcctccag gcagccaagg agcagaagga agtccagacc ccaaagtgga tcacaccagt
     7981 gttgctcctg attgatttct atgaaaagac agccatctcc tcaaaaagga gagcccagat
     8041 gactaagtac ctgcaatcca acagcaacaa ctggcgctgg tttgatgatc gctctgggcg
     8101 ttggtgtagt tacagtgcaa gcaacaatag cactattgat tctgcctgga aatctggaga
     8161 gacaagcgtg cgattcactg caggccgaag aagatacacg gtccaattca ctacaatggt
     8221 gcaggttaat gaggaaacag ggaaccgacg ccctgtgatg ctgactctcc tcagggtacc
     8281 tcggctgaat aaaaattcaa aaaacagcaa tggacaggaa ctagagaaga cgctggaaga
     8341 aagcaaagaa atggatatca aacgtaaaga aaataaaggc aatgataccc ctttggccct
     8401 agagagtaca aacactgaaa aggagacaag cctggaggaa acaaaaatcg gggagatcct
     8461 gatccagggc ttgacagaag atatggtgac tgttttaatc cgggcctgcg tgagcatgct
     8521 gggagtccct gtggacccag atactttgca tgccaccctt cgtctctgtc tgaggctcac
     8581 ccgggaccac aaatatgcca tgatgtttgc agaactgaag agtacccgca tgatcttgaa
     8641 tttgacccag agctcaggct tcaatgggtt tactcccctg gtcacccttc tcttaagaca
     8701 catcattgag gacccctgta cccttcgtca taccatggaa aaggttgttc gctcagcagc
     8761 tacaagtgga gctggtagca ctacctctgg tgttgtgtct ggcagcctcg gctctcggga
     8821 gatcaactac atccttcgtg tccttgggcc agccgcatgc cgcaatccag acatattcac
     8881 agaagtggcc aactgctgta tccgcatcgc ccttcctgcc cctcgaggct caggaactgc
     8941 ttcagatgat gaatttgaga atcttagaat taaaggccct aatgctgtac agctggtgaa
     9001 gaccacccct ttgaagccct cacctctgcc tgtcatccct gatactatca aggaagtgat
     9061 ctatgatatg ctgaatgctc tggctgcata ccatgctcca gaggaagccg ataaatctga
     9121 tcctaaacct ggggttatga cccaagaggt tggccagctc ctgcaagaca tgggtgatga
     9181 tgtataccag cagtaccggt cacttacgcg tcagagcagt gactttgata cgcagtcagg
     9241 tttttccatt aatagtcagg tctttgctgc agatggtgcc tccactgaga cttccgcatc
     9301 tgggacctcc caaggagagg cttcaactcc agaggagtct cgagatggga agaaagataa
     9361 agaaggggac cgggcctctg aggaaggcaa acagaaaggc aagggcagca aacctttaat
     9421 gcctacctcc actatccttc gtcttctggc agagttggtg aggtcctatg ttggtattgc
     9481 taccctgatt gccaactaca gctacactgt gggccagtct gaactgatca aagaggactg
     9541 cagtgtgcta gcttttgttc tggaccacct gctcccacat acccagaatg cagaagacaa
     9601 ggacacccct gccttggccc gcctgttcct cgcaagcctg gctgctgcag ggagtggcac
     9661 agatgcccag gtggccctag tgaatgaagt aaaagcagcc cttggacggg cactggctat
     9721 ggctgagagt acagagaaac atgccaggct tcaggcagtg atgtgtatca tcagtactat
     9781 catggagtcc tgcccctcca cctccagctt ctacagcagt gccacagcga agacccagca
     9841 caatggcatg aacaacatca ttcggctttt cctgaagaag ggactggtta atgacctggc
     9901 cagagtacct cacagcttag acctgtccag tcccaacatg gccaacacag tcaatgctgc
     9961 tctgaagcct ttggaaacac tttcccggat tgtgaaccag cccagtagcc tttttggcag
    10021 caagagtgct tctagcaaga acaagtctga gcaggatgcc caaggagcct ctcaagattc
    10081 cagtagcaac cagcaggacc caggcgagcc tggggaagca gaagtgcagg aggaggatca
    10141 tgatgtcact cagacagagg tggcagatgg ggatatcatg gatggggagg ctgaaaccga
    10201 ctcagtggtg attgctgggc agcctgaggt gctcagttca caagagatgc aggttgagaa
    10261 tgagctggag gacctgatag atgagttgct tgagagggat ggcggatctg ggaacagtac
    10321 aattatagtg agcagaagtg gagaggatga atcacaagag gacgtgctga tggatgaagc
    10381 tccttccaac ctcagccaag cttccacctt gcaggccaac cgagaagatt ccatgaatat
    10441 cctggaccct gaggatgagg aggagcacac tcaggaagag gacagcagtg gcagtaacga
    10501 ggatgaggat gatagtcagg atgaagagga ggaggaggag gaagatgagg aagatgatca
    10561 ggaggatgat gaaggtgaag agggagatga agacgatgac gacgatggct ctgagatgga
    10621 attggatgag gattatcctg atatgaacgc ttctcccttg gtccgatttg agcgctttga
    10681 ccgggaggat gatctcatca ttgagtttga caacatgttc tccagtgcta cagacatccc
    10741 cccatcccca ggaaatatcc ctaccaccca tccactgatg gtgcgccatg cagaccacag
    10801 ttctctgaca ctgggcagtg gctcttcaac aactcgtctc acccagggca tcgggcgcag
    10861 tcagaggacc ctaaggcagc tgacggccaa tactggccac accattcatg ttcactaccc
    10921 tgggaatcgc cagcccaacc ctcctcttat actgcagagg ttgcttggtc cctcagctgc
    10981 tgctgacatc cttcagctga gcagcagcct tcccctacaa agccggggtc gggcccgcct
    11041 cctggtaggc aacgatgacg tccacatcat cgcccgttct gatgatgagc tgctggatga
    11101 ctttttccat gatcagagca cagctaccag ccaagcagga accctgtcca gcatccccac
    11161 agccctgacc cgctggacag aagaatgcaa agttctcgat gctgagagca tgcatgactg
    11221 tgtttcagtg gttaaagtgt ccattgtcaa tcacctggaa ttcctgaggg atgaggagct
    11281 ggaagaaagg cgagagaagc gcaggaaaca actggctgag gaagaaacaa agataactga
    11341 taaaggcaaa gaagataagg agaacaggga tcagagtgca cagtgtactg catctaagtc
    11401 aaatgactcc actgaacaga atctctcaga tgggacgcct atgcctgaca gctacccaac
    11461 aaccccatct tcaactgatg cagctacatc tgagtccaag gagacccttg gcactctgca
    11521 atcctcacaa cagcaaccaa cactcccaac cccaccagct ttgggagagg ttcctcagga
    11581 gctgcagtct ccagctggag aagggggcag ctctacacag ctattgatgc ctgtagagcc
    11641 agaggaattg ggtcccacaa ggccaagtgg ggaagcagaa acaactcaga tggagttatc
    11701 cccagctccc actataacct cactttcccc agagagagct gaggattctg atgcactgac
    11761 ggctgtcagc agtcagctag aaggctctcc tatggataca agcagcctgg cttcctgtac
    11821 cttagaggag gctgtgggtg acacttcagc agctggcagt tctgagcagc ccagagcagg
    11881 cagctccact cctggggatg ccccaccagc tgtggcggaa gtgcaaggca ggagtgatgg
    11941 gtcaggggaa tctgcccagc cacctgagga cagctcccca cctgcatcct ctgagagctc
    12001 ttccaccaga gattctgccg tggccatttc tggcgccgat tcccgaggaa tcctagaaga
    12061 gccgttgcct tcaacaagca gtgaagaaga agatcccctt gcgggtatca gtctccctga
    12121 aggtgtggac ccctcttttc tggctgccct gcctgatgac atccgtcggg aagttctaca
    12181 gaaccagcta ggcattcgtc caccaacccg gactgccccc tccacaaata gctcagcgcc
    12241 tgcagtggtg gggaatcctg gtgtgactga ggtgagtccc gagtttcttg cggcactgcc
    12301 acccgcgatc caagaggaag tactggcaca gcagagagct gagcagcagc gacgagaact
    12361 agcacagaat gccagctcag acacccctat ggaccctgtg accttcatcc agactctgcc
    12421 ctcagacctg cgccgtagtg tcctagagga tatggaggac agtgtgttag ctgtgatgcc
    12481 acctgacatt gcagctgagg ctcaagccct gagacgagag caagaagccc ggcagcgaca
    12541 gctcatgcat gagcgtctgt ttgggcacag tagcacctcc gcactctctg ctattctccg
    12601 aagcccggct ttcaccagtc gcttaagtgg caaccgtggg gtccagtata ctcgccttgc
    12661 tgtgcagaga ggtggcacct tccagatggg gggtagcagc agccataaca ggccttctgg
    12721 cagtaatgta gatactctcc tccgcctccg aggacggctc cttctggacc acgaagccct
    12781 ttcttgtctc ttggtcctac tttttgtgga tgagccaaag ctcaatacta gccgtctaca
    12841 ccgagtactg agaaatctct gctaccatgc ccagacccgc cactgggtca tccgcagtct
    12901 gctctccatc ttgcagcgca gcagtgagag tgagctatgc attgaaacac ccaaactcac
    12961 tacaagtgag gaaaagggca aaaagtcgag caagagctgt gggtcaagta gccatgagaa
    13021 ccgtcccctg gacctgctac acaagatgga gtcaaagagc tccaaccagc tttcctggct
    13081 ctcagtatcc atggatgcag ccctaggctg caggactaat atatttcaga tccagcgttc
    13141 aggggggcgt aaacataccg agaagcatgc aagcggtggc tccaccgtcc acatccatcc
    13201 ccaagctgct cctgttgtct gcagacacgt tttggataca ctcattcaat tggccaaggt
    13261 atttcccagc cacttcacac agcagcggac caaagaaaca aactgtgaga gtgatcggga
    13321 aaggggcaat aaggcctgta gcccatgctc ctcacagtcc tccagcagtg gcatttgcac
    13381 agacttctgg gacttattgg taaaactgga caacatgaat gtcagccgga aaggcaagaa
    13441 ctccgtgaag tcagtgccag tgagcgctgg cggtgagggg gaaacctctc catacagcct
    13501 cgaggcctct ccactggggc agctcatgaa catgttgtca cacccagtca tccgccggag
    13561 ctctctctta actgagaaac tcctcagact cctttctctc atctcaattg ctctcccaga
    13621 aaacaaggtg tcagaagcac aggctaattc tggcagcggt gcttcctcca ccaccactgc
    13681 cacctcaacc acatctacca ccaccaccac tgccgcctcc accacgccca caccccctac
    13741 tgcacccacc cctgtcactt ctgctccagc cctggttgct gccacggcta tttccaccat
    13801 tgtcgtagct gcttcgacca cagtgactac ccccacgact gctaccacta ctgtttcaat
    13861 ttctcccact actaagggca gcaaatctcc agcgaaggtg agtgatgggg gcagcagcag
    13921 tacagacttt aagatggtgt cctctggcct cactgaaaac cagctacagc tctctgtaga
    13981 ggtgttgaca tcccactctt gttctgagga aggcttagag gatgcagcca acgtactact
    14041 gcagctctcc cggggggact ctgggacccg ggacactgtt ctcaagctgc tactgaatgg
    14101 agcccgccat ctgggttata ccctttgtaa acaaataggt accctgctgg ccgagctgcg
    14161 ggaatacaac ctcgagcagc agcggcgagc ccaatgtgaa accctctctc ctgatggcct
    14221 gcctgaggag cagccacaga ccaccaagct gaagggcaaa atgcagagca ggtttgacat
    14281 ggctgagaat gtggtaattg tggcatctca gaagcgacct ttgggtggcc gggagctcca
    14341 gctgccttct atgtccatgt tgacatccaa gacatctacc cagaagttct tcttgagggt
    14401 actacaggtc atcatccagc tccgggacga cacgcgccgg gctaacaaga aagccaagca
    14461 gacaggcagg ctaggttcct ccggtttagg ctcagctagc agcatccagg cagctgttcg
    14521 gcagctggag gctgaggctg atgccattat acaaatggta cgtgagggtc aaagggcgcg
    14581 gagacagcaa caagcagcaa cgtcggagtc tagccagtca gaggcgtctg tccggaggga
    14641 ggaatcaccc atggatgtgg accagccatc tcccagtgct caagatactc aatccattgc
    14701 ctccgatgga accccacagg gggagaagga aaaggaagaa agaccacctg agttacccct
    14761 gctcagcgag cagctgagtt tggacgagct gtgggacatg cttggggagt gtctaaagga
    14821 actagaggaa tcccatgacc agcatgcggt gctagtgcta cagcctgctg tcgaggcctt
    14881 ctttctggtc catgccacag agcgggagag caagcctcct gtccgagaca cccgtgagag
    14941 ccagctggca cacatcaagg acgagcctcc tccactctcc cctgccccct taaccccagc
    15001 cacgccttcc tcccttgacc cattcttctc ccgggagccc tcatctatgc acatctcctc
    15061 aagcctgccc cctgacacac agaagttcct tcgctttgca gagactcacc gcactgtgtt
    15121 aaaccagatc ctacggcagt ccacgaccca ccttgctgat gggccttttg ctgtcctggt
    15181 agactacatt cgtgtcctcg actttgatgt caagcgcaaa tatttccgcc aagagctgga
    15241 gcgtttagat gaggggctcc ggaaagaaga catggctgtg catgtccgtc gtgaccatgt
    15301 gtttgaagac tcctatcgtg agctgcatcg caaatccccc gaagaaatga agaatcgatt
    15361 gtatatagta tttgaaggag aagaagggca ggatgctggc gggctcctgc gggagtggta
    15421 tatgatcatc tctcgagaga tgtttaaccc tatgtatgcc ttgttccgta cctcacctgg
    15481 tgatcgagtc acctacacca tcaatccatc ttcccactgc aaccccaacc atctgtccta
    15541 ttttaaattt gtcggacgca ttgtggccaa agctgtatat gacaaccgtc ttctggagtg
    15601 ctactttact cgatcctttt acaaacacat cttgggcaag tcagtcagat atacagatat
    15661 ggagagtgaa gattaccact tctaccaagg tctggtttat ctgctggaaa atgatgtctc
    15721 cacactaggc tatgacctca ccttcagcac tgaggtccaa gagtttggag tttgtgaagt
    15781 tcgtgacctc aaacccaatg gggccaacat cttggtaaca gaggagaata agaaggagta
    15841 tgtacacctg gtatgccaga tgagaatgac aggagccatc cgcaagcagt tggcggcttt
    15901 cttagaaggc ttctatgaga tcattccaaa gcgcctcatt tccatcttca ctgagcagga
    15961 gttagagctg cttatatcag gactgcccac cattgacatc gatgatctga aatccaacac
    16021 tgaataccac aagtaccagt ccaactctat tcagatccag tggttctgga gagcattgcg
    16081 ttctttcgat caagctgacc gtgccaagtt cctccagttt gtcacgggta cttccaaggt
    16141 acccctgcaa ggctttgctg ccctcgaagg catgaatggc attcagaagt ttcagatcca
    16201 tcgagatgac aggtccacag atcgcctgcc ttcagctcac acatgtttta atcagctgga
    16261 tctgcctgcc tatgagagct ttgagaagct ccgccacatg ctactgttgg ctatccagga
    16321 gtgctctgaa ggctttgggc tggccagcgg ccgccttgag gtgctttttc aaggtccggg
    16381 cagcggtggc ggctctgatt ataaggatca cgatggagac tacaaggacc acgatatcga
    16441 ctataaagac gatgacgata aatgatgata atggcctgtg gccgtaatgg cctgtggccg
    16501 taaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    16561 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    16621 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    16681 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    16741 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    16801 cctattgcca cggcggaact cagtgccgcc tgccttgccc gctgctggac aggggctcgg
    16861 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    16921 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    16981 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgg
    17041 cttcgagatc tgcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
    17101 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
    17161 aaattgcatc aaattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
    17221 acagcaaggg ggaggattgg gcagacaata gcaggcatgc tggggactcg agttaagggc
    17281 gaatcgctgg gacgcccgaa cggtggctac tcgttgacat aagcctgttc ggttcgtaaa
    17341 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
    17401 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct
    17461 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
    17521 agcagcaacg atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac
    17581 aaagttaggt ggctcaagta tgggcatcat tcgtacatgt aggctcggcc ctgaccaagt
    17641 caaatccatg cgggctgctc ttgatctttt cggtcgtgag ttcggtgatg tagccaccta
    17701 ctcccaacat cagccggact ccgattacct cgggaacttg ctccgtagta agacatttat
    17761 tgcgcttgct gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc
    17821 caagtttgag cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca
    17881 ccggaggcag ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct
    17941 tggtgcttat gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta
    18001 tacaaagttg ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac
    18061 ctaacaattc gttcaagccg agatcggctt cccggccgcg gagttgttcg gtaaattgtc
    18121 acaacgccgc
//
