LOCUS       sequence-369462-       13673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human EGFR labeled with CD3-epsilon ITAMs for monitoring with 
            CD3-epsilon/ZtSH2 pYtag biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR EGFR-CD3-epsilon-FusionRed
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             220..264
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    549..666
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        819..1226
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(1292..1363,1364..3226,3227..3295,3296..4921)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             5231..5926
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    6036..6624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6089..6109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6507..6518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6627..6643)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6628..6644)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6734..6967
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6992..7010)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6993..7010)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7300..7319)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7533..7552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7652..7674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7712..7730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7798..7902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7903..7971,7972..8763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8934..9522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9423..9442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9676..9693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9763..9783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9768..10097
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9948..10083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11231..11296
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11426..11446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11871..12005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11908..11927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11962..11981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12173..12806
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12853..12978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(13475..13673,1..35)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
       61 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
      121 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
      181 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
      241 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
      301 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
      361 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
      421 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
      481 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
      541 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      601 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      661 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
      721 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
      781 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
      841 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
      901 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
      961 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     1021 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     1081 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     1141 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     1201 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
     1261 aagtggagca aggcaggtgg acagtggatc catgcgaccc tccgggacgg ccggggcagc
     1321 gctcctggcg ctgctggctg cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt
     1381 ttgccaaggc acgagtaaca agctcacgca gttgggcact tttgaagatc attttctcag
     1441 cctccagagg atgttcaata actgtgaggt ggtccttggg aatttggaaa ttacctatgt
     1501 gcagaggaat tatgatcttt ccttcttaaa gaccatccag gaggtggctg gttatgtcct
     1561 cattgccctc aacacagtgg agcgaattcc tttggaaaac ctgcagatca tcagaggaaa
     1621 tatgtactac gaaaattcct atgccttagc agtcttatct aactatgatg caaataaaac
     1681 cggactgaag gagctgccca tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt
     1741 cagcaacaac cctgccctgt gcaacgtgga gagcatccag tggcgggaca tagtcagcag
     1801 tgactttctc agcaacatgt cgatggactt ccagaaccac ctgggcagct gccaaaagtg
     1861 tgatccaagc tgtcccaatg ggagctgctg gggtgcagga gaggagaact gccagaaact
     1921 gaccaaaatc atctgtgccc agcagtgctc cgggcgctgc cgtggcaagt cccccagtga
     1981 ctgctgccac aaccagtgtg ctgcaggctg cacaggcccc cgggagagcg actgcctggt
     2041 ctgccgcaaa ttccgagacg aagccacgtg caaggacacc tgccccccac tcatgctcta
     2101 caaccccacc acgtaccaga tggatgtgaa ccccgagggc aaatacagct ttggtgccac
     2161 ctgcgtgaag aagtgtcccc gtaattatgt ggtgacagat cacggctcgt gcgtccgagc
     2221 ctgtggggcc gacagctatg agatggagga agacggcgtc cgcaagtgta agaagtgcga
     2281 agggccttgc cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc
     2341 cataaatgct acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca
     2401 catcctgccg gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca
     2461 ggaactggat attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg
     2521 gcctgaaaac aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac
     2581 caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt
     2641 acgctccctc aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg
     2701 ctatgcaaat acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat
     2761 tataagcaac agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg
     2821 ctcccccgag ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag
     2881 ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt
     2941 ggagaactct gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac
     3001 ctgcacagga cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca
     3061 ctgcgtcaag acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta
     3121 cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg
     3181 gccaggtctt gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat
     3241 ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgcgaag
     3301 gcgccacatc gttcggaagc gcacgctgcg gaggctgctg caggagaggg agcttgtgga
     3361 gcctcttaca cccagtggag aagctcccaa ccaagctctc ttgaggatct tgaaggaaac
     3421 tgaattcaaa aagatcaaag tgctgggctc cggtgcgttc ggcacggtgt ataagggact
     3481 ctggatccca gaaggtgaga aagttaaaat tcccgtcgct atcaaggaat taagagaagc
     3541 aacatctccg aaagccaaca aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga
     3601 caacccccac gtgtgccgcc tgctgggcat ctgcctcacc tccaccgtgc aactcatcac
     3661 gcagctcatg cccttcggct gcctcctgga ctatgtccgg gaacacaaag acaatattgg
     3721 ctcccagtac ctgctcaact ggtgtgtgca gatcgcaaag ggcatgaact acttggagga
     3781 ccgtcgcttg gtgcaccgcg acctggcagc caggaacgta ctggtgaaaa caccgcagca
     3841 tgtcaagatc acagattttg ggctggccaa actgctgggt gcggaagaga aagaatacca
     3901 tgcagaagga ggcaaagtgc ctatcaagtg gatggcattg gaatcaattt tacacagaat
     3961 ctatacccac cagagtgatg tctggagcta cggggtgacc gtttgggagt tgatgacctt
     4021 tggatccaag ccatatgacg gaatccctgc cagcgagatc tcctccatcc tggagaaagg
     4081 agaacgcctc cctcagccac ccatatgtac catcgatgtc tacatgatca tggtcaagtg
     4141 ctggatgata gacgcagata gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa
     4201 aatggcccga gacccccagc gctaccttgt cattcagggg gatgaaagaa tgcatttgcc
     4261 aagtcctaca gactccaact tctaccgtgc cctgatggat gaagaagaca tggacgacgt
     4321 ggtggatgcc gacgagtacc tcatcccaca gcagggcttc ttcagcagcc cctccacgtc
     4381 acggactccc ctcctgagct ctctgagtgc aaccagcaac aattccaccg tggcttgcat
     4441 tgatagaaat gggctgcaaa gctgtcccat caaggaagac agcttcttgc agcgatacag
     4501 ctcagacccc acaggcgcct tgactgagga cagcatagac gacaccttcc tcccagtgcc
     4561 tgaatacata aaccagtccg ttcccaaaag gcccgctggc tctgtgcaga atcctgtcta
     4621 tcacaatcag cctctgaacc ccgcgcccag cagagaccca cactaccagg acccccacag
     4681 cactgcagtg ggcaaccccg agtatctcaa cactgtccag cccacctgtg tcaacagcac
     4741 attcgacagc cctgcccact gggcccagaa aggcagccac caaattagcc tggacaaccc
     4801 tgactaccag caggacttct ttcccaagga agccaagcca aatggcatct ttaagggctc
     4861 cacagctgaa aatgcagaat acctaagggt cgcgccacaa agcagtgaat ttattggagc
     4921 aggctctggt tctccacctc ccgtccctaa tcccgattat gagcccattc ggaaaggtca
     4981 aagagatttg tatagtggcc tcaatcaaag aagaatcggg tcaggttccg gcccgccgcc
     5041 agtcccaaac cccgactatg aaccaatcag gaaaggccaa cgggatttgt actcaggatt
     5101 gaatcagcgc aggataggtt ccggctccgg gccaccaccc gtccctaacc cagattacga
     5161 acccatacgc aagggacaac gcgatcttta cagcggattg aaccaacgac gaataggatc
     5221 tggttctggc atggtgagcg agctgattaa ggagaacatg cccatgaagc tgtacatgga
     5281 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     5341 gggcacccag accatgagaa tcaaggtcgt cgagggcggc cctctcccct tcgccttcga
     5401 catcctggct accagcttca tgtacggcag cagaaccttc atcaagcacc ctccgggcat
     5461 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     5521 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     5581 ctacaacgtc aaggttagag gggtgaactt cccagccaac ggccctgtga tgcagaagaa
     5641 aacactcggc tgggaggcct ccaccgagac gatgtacccc gctgacggcg gcctggaagg
     5701 cgcatgtgac atggccctga agctcgtggg cgggggccac ctgatctgca accttgagac
     5761 cacatacaga tccaagaaac ccgctacgaa cctcaagatg cccggcgtct acaacgtgga
     5821 ccacagactg gaaagaatca aggaggccga cgatgagacc tacgtcgagc agcacgaggt
     5881 ggctgtggcc agatactcta ctggtggcgc tggtgatgga ggtaaagcgg ccacgcgtgc
     5941 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
     6001 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     6061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6601 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc
     6661 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6721 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6781 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6961 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     7021 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     7081 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     7141 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     7201 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     7261 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     7321 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     7381 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     7441 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     7501 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7561 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     7621 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     7681 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7741 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7921 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7981 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8041 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8101 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8161 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8221 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8281 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8341 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8401 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8461 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8521 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8581 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8641 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8701 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8761 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8821 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     9781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10081 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
    10141 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
    10201 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
    10261 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
    10321 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
    10381 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
    10441 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
    10501 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
    10561 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
    10621 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
    10681 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
    10741 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
    10801 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
    10861 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
    10921 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
    10981 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
    11041 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
    11101 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
    11161 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    11221 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
    11281 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
    11341 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
    11401 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
    11461 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
    11521 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
    11581 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
    11641 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
    11701 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
    11761 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    11821 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    11881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12001 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttccaacc ccatacccta
    12061 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    12121 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    12181 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    12241 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    12301 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    12361 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    12421 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    12481 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    12541 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    12601 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    12661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    12721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    12781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    12841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    12901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    12961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    13021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    13081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    13141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    13201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    13261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    13321 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    13381 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    13441 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    13501 gagcagcagg aagcactata ggcgcagcgt caatgacgct gacggtacag gccagacaat
    13561 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    13621 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctg
//
