LOCUS       sequence-369463-       17897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression FLAG-HUWE C4341S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 17897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDEST_FLAG-HUWE1_C4341S
FEATURES             Location/Qualifiers
     source          1..17897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    995..1019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14180..14204)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    14238..14860
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14437..14456)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14884..14904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14889..15218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15069..15204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(15256..15275)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15257..15275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15289..15305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15289..15305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15302..15324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15313..15329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15337..15343,15344..15361,15362..15367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15382..15403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15520..15537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15691..16279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15771..15790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16450..17241,17242..17310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17073..17092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17311..17415)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      17442..17870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17529..17548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17739..17760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatctgat atcaacaagt ttgtacaaaa aagcaggctt
     1021 taaaggaacc aattcagtcg acatgaaagt agacaggact aaactgaaga agacacctac
     1081 tgaggctcct gcagactgca gagccttaat agacaaactc aaagtttgta atgatgagca
     1141 acttctcttg gaactgcagc agatcaaaac atggaacatt ggaaagtgcg agttatatca
     1201 ctgggtggac ctgttggacc gcttcgatgg aatactggca gatgctggac agacagtgga
     1261 gaatatgtca tggatgctcg tatgtgatag gccagaaaga gagcaactga aaatgcttct
     1321 cttggctgtg ttgaacttca cagccttgct cattgagtac agcttttccc ggcatctgta
     1381 cagttccata gagcatttga caactttatt ggcttcctct gatatgcaag tggtgctggc
     1441 agtcctcaat ctcctatatg tatttagcaa aagatcaaac tacatcactc gtctgggatc
     1501 tgacaagagg accccgctgc taactcggct acaacatttg gcagagagct ggggtggaaa
     1561 ggagaatggc tttggacttg cagaatgttg cagagacttg catatgatga aatatccacc
     1621 cagtgcaact acactacact ttgaattcta tgcagatcct ggggccgagg tcaaaattga
     1681 gaaaaggaca actagtaaca cactacatta tattcacata gagcaacttg acaagatttc
     1741 agaaagccct tctgaaatca tggaatctct taccaaaatg tacagcattc ctaaggataa
     1801 gcagatgctg ttatttacac acatacgact ggcccatggc ttttctaatc acaggaagcg
     1861 attgcaggca gttcaggcca gactgcatgc aatatctata ttagtgtatt ccaatgcctt
     1921 gcaggaatca gcaaacagta tcttgtataa tggcttgata gaggagttag tggacgtgct
     1981 gcaaataacg gataagcagc ttatggagat taaagcagct tctttacgaa cattaacatc
     2041 aattgtccac ttggagagaa ctcccaaact cagcagtatt attgactgta ctggaactgc
     2101 ctcctaccat ggatttttgc cagtgcttgt aaggaactgt atccaggcca tgattgatcc
     2161 ttccatggat ccataccctc accagtttgc cactgctctc ttctcttttt tataccatct
     2221 ggccagctac gatgctggtg gtgaagcctt ggtctcctgt ggaatgatgg aagccttatt
     2281 gaaggtcata aagtttcttg gcgatgaaca ggaccagata acatttgtca ccagagccgt
     2341 cagagtggtt gaccttatca ccaacctgga tatggcagct tttcaatccc atagtggact
     2401 ttctatcttc atttatagac ttgagcatga agtagatctg tgccgaaaag aatgtccgtt
     2461 tgtgatcaag ccaaagatcc agagacccaa tactacacaa gaaggagagg aaatggaaac
     2521 tgatatggat ggagtccagt gtattccaca acgagcagca cttctgaaat ccatgttgaa
     2581 tttcctcaag aaggccatcc aagaccctgc tttctcagat ggcatacgac atgtgatgga
     2641 tggttctctg cctacctccc tgaaacacat catcagcaat gcagaatact atggcccatc
     2701 actcttcctc ctagctactg aagtggtgac tgtgtttgta tttcaagaac catcactgct
     2761 ctcctcactc caggacaatg gattgacaga tgtcatgctg catgcactgc ttatcaaaga
     2821 tgttcctgct acccgtgaag tccttggctc cctcccaaat gtattcagtg cactctgttt
     2881 gaatgcccga ggtcttcagt cttttgttca gtgtcagcct tttgaacgcc tcttcaaagt
     2941 tcttctgtct ccagattacc tcccagccat gcggaggagg agaagttctg atccccttgg
     3001 ggatactgca tccaacctgg ggagtgctgt cgatgagctc atgagacatc agcccaccct
     3061 taaaacagat gcaacgactg ccatcatcaa gttacttgaa gaaatctgta atcttggaag
     3121 ggaccccaaa tacatctgtc agaagccatc gatccagaag gcagatggca ctgccactgc
     3181 tcctccccca aggtctaatc atgccgcaga agaagcctct agtgaggatg aggaggaaga
     3241 ggaagtacag gccatgcaga gctttaattc tacccagcaa aatgaaactg agcctaatca
     3301 gcaggttgtt ggtacagagg aacgtattcc tattcccctc atggattaca tccttaatgt
     3361 gatgaaattt gtggaatcta ttctgagcaa caatacaaca gatgaccact gccaggaatt
     3421 tgtgaatcag aaaggactgt tgcctttggt taccattttg ggtcttccca atctgcccat
     3481 tgactttccc acatctgctg cctgtcaggc tgttgcaggt gtctgcaaat ccatattgac
     3541 actgtcacat gaacccaaag tccttcaaga gggtctcctt cagttggact ccatcctctc
     3601 ctccctggag cccttacacc gccccattga atcccctggg ggctcagtgt tgttgcgaga
     3661 actggcttgc gcaggcaatg ttgctgatgc taccctctca gcccaggcca cacctctgct
     3721 gcatgcactc actgctgccc atgcctacat catgatgttt gttcatactt gcagagttgg
     3781 acagagtgaa attcgttcca tctccgtaaa ccagtggggc tctcaattgg gtctgagtgt
     3841 tttgagcaag ctgagccagt tatactgttc cctggtgtgg gaaagcactg tcctcctctc
     3901 tctgtgtacc ccaaacagcc taccatctgg gtgtgaattt ggccaggcag atatgcagaa
     3961 actggttcca aaggatgaga aggcaggtac gacccagggc ggaaaaagat cagatgggga
     4021 acaggatgga gcagctggaa gtatggatgc ttctacccag ggcttattag aaggcattgg
     4081 gctagatggt gacacattgg ctcccatgga gacagatgaa cctactgctt cagactctaa
     4141 gggcaaatct aaaatcacac cagcaatggc tgccagaatt aagcaaatca agcctttgtt
     4201 atcagcttcc tccagattag gccgagcact tgctgagcta tttggacttc ttgttaaact
     4261 ttgtgtggga tctcctgtcc gccagagaag gagccatcat gctgccagca ccactacagc
     4321 accgacacct gccgcgcgat caacagcctc agctctcact aagctcttga ctaaggggtt
     4381 atcttggcag cccccaccat atacacctac tccccgattc aggctgacat tcttcatctg
     4441 ttcagttggt ttcacatccc caatgctgtt tgatgagagg aagtatccct accacctcat
     4501 gctgcaaaaa tttctctgct ccggaggcca caatgctctt tttgaaactt tcaactgggc
     4561 tctgtccatg ggaggtaaag ttcctgtttc tgagggattg gaacactcag acttgcctga
     4621 tggcacagga gaattcctag atgcctggct tatgctggtg gagaagatgg tgaatcccac
     4681 cacggtgctt gaatctccac attcgctgcc tgccaaattg cctggaggtg tccagaactt
     4741 tccccagttc agtgcactgc gcttccttgt ggtaactcag aaagcagcct ttacttgcat
     4801 caaaaactta tggaaccgga aacccctgaa ggtatatggt ggacgaatgg ctgaatcgat
     4861 gctggccatt ctatgccaca tcctccgagg agaacctgtg attcgagaga gactaagcaa
     4921 ggagaaggag gggtctcgag gagaagagga tacagggcaa gaggaaggtg gctcccgccg
     4981 ggaacctcaa gtcaaccagc aacaactgca acagctcatg gacatgggct tcacaaggga
     5041 acatgcaatg gaggcactgt tgaacaccag caccatggag caggccacag agtacctttt
     5101 aacccaccct cctccaatca tgggaggagt tgttcgggat ctcagcatgt ctgaagagga
     5161 ccagatgatg agagcaattg ctatgtctct gggacaggat attccaatgg atcaaagggc
     5221 agagtcacct gaggaagttg cttgccggaa ggaggaagag gaacggaaag ctcgggaaaa
     5281 gcaggaggag gaagaggcta aatgtctaga gaagttccag gatgctgacc cgttggaaca
     5341 agatgagctc cacactttca cagatactat gttgccaggc tgcttccacc ttcttgatga
     5401 gctgccagac acagtatacc gtgtgtgtga cctgatcatg acagcaatca aacgtaatgg
     5461 agcagattat cgtgacatga ttctgaagca agtagtcaat caggtgtggg aagctgctga
     5521 tgtattgatc aaagctgctc ttcccctgac aacaagtgac acaaaaaccg tgtcagagtg
     5581 gataagtcag atggccacac tgccccaggc ctccaatttg gctactagaa tcttgctttt
     5641 aacgctactt tttgaggagt tgaagctacc ttgtgcttgg gtggttgaat caagtggcat
     5701 ccttaatgtc ctaatcaaac tcttggaagt ggttcagccc tgcctccagg cagccaagga
     5761 gcagaaggaa gtccagaccc caaagtggat cacaccagtg ttgctcctga ttgatttcta
     5821 tgaaaagaca gccatctcct caaaaaggag agcccagatg actaagtacc tgcaatccaa
     5881 cagcaacaac tggcgctggt ttgatgatcg ctctgggcgt tggtgtagtt acagtgcaag
     5941 caacaatagc actattgatt ctgcctggaa atctggagag acaagcgtgc gattcactgc
     6001 aggccgaaga agatacacgg tccaattcac tacaatggtg caggttaatg aggaaacagg
     6061 gaaccgacgc cctgtgatgc tgactctcct cagggtacct cggctgaata aaaattcaaa
     6121 aaacagcaat ggacaggaac tagagaagac gctggaagaa agcaaagaaa tggatatcaa
     6181 acgtaaagaa aataaaggca atgatacccc tttggcccta gagagtacaa acactgaaaa
     6241 ggagacaagc ctggaggaaa caaaaatcgg ggagatcctg atccagggct tgacagaaga
     6301 tatggtgact gttttaatcc gggcctgcgt gagcatgctg ggagtccctg tggacccaga
     6361 tactttgcat gccacccttc gtctctgtct gaggctcacc cgggaccaca aatatgccat
     6421 gatgtttgca gaactgaaga gtacccgcat gatcttgaat ttgacccaga gctcaggctt
     6481 caatgggttt actcccctgg tcacccttct cttaagacac atcattgagg acccctgtac
     6541 ccttcgtcat accatggaaa aggttgttcg ctcagcagct acaagtggag ctggtagcac
     6601 tacctctggt gttgtgtctg gcagcctcgg ctctcgggag atcaactaca tccttcgtgt
     6661 ccttgggcca gccgcatgcc gcaatccaga catattcaca gaagtggcca actgctgtat
     6721 ccgcatcgcc cttcctgccc ctcgaggctc aggaactgct tcagatgatg aatttgagaa
     6781 tcttagaatt aaaggcccta atgctgtaca gctggtgaag accacccctt tgaagccctc
     6841 acctctgcct gtcatccctg atactatcaa ggaagtgatc tatgatatgc tgaatgctct
     6901 ggctgcatac catgctccag aggaagccga taaatctgat cctaaacctg gggttatgac
     6961 ccaagaggtt ggccagctcc tgcaagacat gggtgatgat gtataccagc agtaccggtc
     7021 acttacgcgt cagagcagtg actttgatac gcagtcaggt ttttccatta atagtcaggt
     7081 ctttgctgca gatggtgcct ccactgagac ttccgcatct gggacctccc aaggagaggc
     7141 ttcaactcca gaggagtctc gagatgggaa gaaagataaa gaaggggacc gggcctctga
     7201 ggaaggcaaa cagaaaggca agggcagcaa acctttaatg cctacctcca ctatccttcg
     7261 tcttctggca gagttggtga ggtcctatgt tggtattgct accctgattg ccaactacag
     7321 ctacactgtg ggccagtctg aactgatcaa agaggactgc agtgtgctag cttttgttct
     7381 ggaccacctg ctcccacata cccagaatgc agaagacaag gacacccctg ccttggcccg
     7441 cctgttcctc gcaagcctgg ctgctgcagg gagtggcaca gatgcccagg tggccctagt
     7501 gaatgaagta aaagcagccc ttggacgggc actggctatg gctgagagta cagagaaaca
     7561 tgccaggctt caggcagtga tgtgtatcat cagtactatc atggagtcct gcccctccac
     7621 ctccagcttc tacagcagtg ccacagcgaa gacccagcac aatggcatga acaacatcat
     7681 tcggcttttc ctgaagaagg gactggttaa tgacctggcc agagtacctc acagcttaga
     7741 cctgtccagt cccaacatgg ccaacacagt caatgctgct ctgaagcctt tggaaacact
     7801 ttcccggatt gtgaaccagc ccagtagcct ttttggcagc aagagtgctt ctagcaagaa
     7861 caagtctgag caggatgccc aaggagcctc tcaagattcc agtagcaacc agcaggaccc
     7921 aggcgagcct ggggaagcag aagtgcagga ggaggatcat gatgtcactc agacagaggt
     7981 ggcagatggg gatatcatgg atggggaggc tgaaaccgac tcagtggtga ttgctgggca
     8041 gcctgaggtg ctcagttcac aagagatgca ggttgagaat gagctggagg acctgataga
     8101 tgagttgctt gagagggatg gcggatctgg gaacagtaca attatagtga gcagaagtgg
     8161 agaggatgaa tcacaagagg acgtgctgat ggatgaagct ccttccaacc tcagccaagc
     8221 ttccaccttg caggccaacc gagaagattc catgaatatc ctggaccctg aggatgagga
     8281 ggagcacact caggaagagg acagcagtgg cagtaacgag gatgaggatg atagtcagga
     8341 tgaagaggag gaggaggagg aagatgagga agatgatcag gaggatgatg aaggtgaaga
     8401 gggagatgaa gacgatgacg acgatggctc tgagatggaa ttggatgagg attatcctga
     8461 tatgaacgct tctcccttgg tccgatttga gcgctttgac cgggaggatg atctcatcat
     8521 tgagtttgac aacatgttct ccagtgctac agacatcccc ccatccccag gaaatatccc
     8581 taccacccat ccactgatgg tgcgccatgc agaccacagt tctctgacac tgggcagtgg
     8641 ctcttcaaca actcgtctca cccagggcat cgggcgcagt cagaggaccc taaggcagct
     8701 gacggccaat actggccaca ccattcatgt tcactaccct gggaatcgcc agcccaaccc
     8761 tcctcttata ctgcagaggt tgcttggtcc ctcagctgct gctgacatcc ttcagctgag
     8821 cagcagcctt cccctacaaa gccggggtcg ggcccgcctc ctggtaggca acgatgacgt
     8881 ccacatcatc gcccgttctg atgatgagct gctggatgac tttttccatg atcagagcac
     8941 agctaccagc caagcaggaa ccctgtccag catccccaca gccctgaccc gctggacaga
     9001 agaatgcaaa gttctcgatg ctgagagcat gcatgactgt gtttcagtgg ttaaagtgtc
     9061 cattgtcaat cacctggaat tcctgaggga tgaggagctg gaagaaaggc gagagaagcg
     9121 caggaaacaa ctggctgagg aagaaacaaa gataactgat aaaggcaaag aagataagga
     9181 gaacagggat cagagtgcac agtgtactgc atctaagtca aatgactcca ctgaacagaa
     9241 tctctcagat gggacgccta tgcctgacag ctacccaaca accccatctt caactgatgc
     9301 agctacatct gagtccaagg agacccttgg cactctgcaa tcctcacaac agcaaccaac
     9361 actcccaacc ccaccagctt tgggagaggt tcctcaggag ctgcagtctc cagctggaga
     9421 agggggcagc tctacacagc tattgatgcc tgtagagcca gaggaattgg gtcccacaag
     9481 gccaagtggg gaagcagaaa caactcagat ggagttatcc ccagctccca ctataacctc
     9541 actttcccca gagagagctg aggattctga tgcactgacg gctgtcagca gtcagctaga
     9601 aggctctcct atggatacaa gcagcctggc ttcctgtacc ttagaggagg ctgtgggtga
     9661 cacttcagca gctggcagtt ctgagcagcc cagagcaggc agctccactc ctggggatgc
     9721 cccaccagct gtggcggaag tgcaaggcag gagtgatggg tcaggggaat ctgcccagcc
     9781 acctgaggac agctccccac ctgcatcctc tgagagctct tccaccagag attctgccgt
     9841 ggccatttct ggcgccgatt cccgaggaat cctagaagag ccgttgcctt caacaagcag
     9901 tgaagaagaa gatccccttg cgggtatcag tctccctgaa ggtgtggacc cctcttttct
     9961 ggctgccctg cctgatgaca tccgtcggga agttctacag aaccagctag gcattcgtcc
    10021 accaacccgg actgccccct ccacaaatag ctcagcgcct gcagtggtgg ggaatcctgg
    10081 tgtgactgag gtgagtcccg agtttcttgc ggcactgcca cccgcgatcc aagaggaagt
    10141 actggcacag cagagagctg agcagcagcg acgagaacta gcacagaatg ccagctcaga
    10201 cacccctatg gaccctgtga ccttcatcca gactctgccc tcagacctgc gccgtagtgt
    10261 cctagaggat atggaggaca gtgtgttagc tgtgatgcca cctgacattg cagctgaggc
    10321 tcaagccctg agacgagagc aagaagcccg gcagcgacag ctcatgcatg agcgtctgtt
    10381 tgggcacagt agcacctccg cactctctgc tattctccga agcccggctt tcaccagtcg
    10441 cttaagtggc aaccgtgggg tccagtatac tcgccttgct gtgcagagag gtggcacctt
    10501 ccagatgggg ggtagcagca gccataacag gccttctggc agtaatgtag atactctcct
    10561 ccgcctccga ggacggctcc ttctggacca cgaagccctt tcttgtctct tggtcctact
    10621 ttttgtggat gagccaaagc tcaatactag ccgtctacac cgagtactga gaaatctctg
    10681 ctaccatgcc cagacccgcc actgggtcat ccgcagtctg ctctccatct tgcagcgcag
    10741 cagtgagagt gagctatgca ttgaaacacc caaactcact acaagtgagg aaaagggcaa
    10801 aaagtcgagc aagagctgtg ggtcaagtag ccatgagaac cgtcccctgg acctgctaca
    10861 caagatggag tcaaagagct ccaaccagct ttcctggctc tcagtatcca tggatgcagc
    10921 cctaggctgc aggactaata tatttcagat ccagcgttca ggggggcgta aacataccga
    10981 gaagcatgca agcggtggct ccaccgtcca catccatccc caagctgctc ctgttgtctg
    11041 cagacacgtt ttggatacac tcattcaatt ggccaaggta tttcccagcc acttcacaca
    11101 gcagcggacc aaagaaacaa actgtgagag tgatcgggaa aggggcaata aggcctgtag
    11161 cccatgctcc tcacagtcct ccagcagtgg catttgcaca gacttctggg acttattggt
    11221 aaaactggac aacatgaatg tcagccggaa aggcaagaac tccgtgaagt cagtgccagt
    11281 gagcgctggc ggtgaggggg aaacctctcc atacagcctc gaggcctctc cactggggca
    11341 gctcatgaac atgttgtcac acccagtcat ccgccggagc tctctcttaa ctgagaaact
    11401 cctcagactc ctttctctca tctcaattgc tctcccagaa aacaaggtgt cagaagcaca
    11461 ggctaattct ggcagcggtg cttcctccac caccactgcc acctcaacca catctaccac
    11521 caccaccact gccgcctcca ccacgcccac accccctact gcacccaccc ctgtcacttc
    11581 tgctccagcc ctggttgctg ccacggctat ttccaccatt gtcgtagctg cttcgaccac
    11641 agtgactacc cccacgactg ctaccactac tgtttcaatt tctcccacta ctaagggcag
    11701 caaatctcca gcgaaggtga gtgatggggg cagcagcagt acagacttta agatggtgtc
    11761 ctctggcctc actgaaaacc agctacagct ctctgtagag gtgttgacat cccactcttg
    11821 ttctgaggaa ggcttagagg atgcagccaa cgtactactg cagctctccc ggggggactc
    11881 tgggacccgg gacactgttc tcaagctgct actgaatgga gcccgccatc tgggttatac
    11941 cctttgtaaa caaataggta ccctgctggc cgagctgcgg gaatacaacc tcgagcagca
    12001 gcggcgagcc caatgtgaaa ccctctctcc tgatggcctg cctgaggagc agccacagac
    12061 caccaagctg aagggcaaaa tgcagagcag gtttgacatg gctgagaatg tggtaattgt
    12121 ggcatctcag aagcgacctt tgggtggccg ggagctccag ctgccttcta tgtccatgtt
    12181 gacatccaag acatctaccc agaagttctt cttgagggta ctacaggtca tcatccagct
    12241 ccgggacgac acgcgccggg ctaacaagaa agccaagcag acaggcaggc taggttcctc
    12301 cggtttaggc tcagctagca gcatccaggc agctgttcgg cagctggagg ctgaggctga
    12361 tgccattata caaatggtac gtgagggtca aagggcgcgg agacagcaac aagcagcaac
    12421 gtcggagtct agccagtcag aggcgtctgt ccggagggag gaatcaccca tggatgtgga
    12481 ccagccatct cccagtgctc aagatactca atccattgcc tccgatggaa ccccacaggg
    12541 ggagaaggaa aaggaagaaa gaccacctga gttacccctg ctcagcgagc agctgagttt
    12601 ggacgagctg tgggacatgc ttggggagtg tctaaaggaa ctagaggaat cccatgacca
    12661 gcatgcggtg ctagtgctac agcctgctgt cgaggccttc tttctggtcc atgccacaga
    12721 gcgggagagc aagcctcctg tccgagacac ccgtgagagc cagctggcac acatcaagga
    12781 cgagcctcct ccactctccc ctgccccctt aaccccagcc acgccttcct cccttgaccc
    12841 attcttctcc cgggagccct catctatgca catctcctca agcctgcccc ctgacacaca
    12901 gaagttcctt cgctttgcag agactcaccg cactgtgtta aaccagatcc tacggcagtc
    12961 cacgacccac cttgctgatg ggccttttgc tgtcctggta gactacattc gtgtcctcga
    13021 ctttgatgtc aagcgcaaat atttccgcca agagctggag cgtttagatg aggggctccg
    13081 gaaagaagac atggctgtgc atgtccgtcg tgaccatgtg tttgaagact cctatcgtga
    13141 gctgcatcgc aaatcccccg aagaaatgaa gaatcgattg tatatagtat ttgaaggaga
    13201 agaagggcag gatgctggcg ggctcctgcg ggagtggtat atgatcatct ctcgagagat
    13261 gtttaaccct atgtatgcct tgttccgtac ctcacctggt gatcgagtca cctacaccat
    13321 caatccatct tcccactgca accccaacca tctgtcctat tttaaatttg tcggacgcat
    13381 tgtggccaaa gctgtatatg acaaccgtct tctggagtgc tactttactc gatcctttta
    13441 caaacacatc ttgggcaagt cagtcagata tacagatatg gagagtgaag attaccactt
    13501 ctaccaaggt ctggtttatc tgctggaaaa tgatgtctcc acactaggct atgacctcac
    13561 cttcagcact gaggtccaag agtttggagt ttgtgaagtt cgtgacctca aacccaatgg
    13621 ggccaacatc ttggtaacag aggagaataa gaaggagtat gtacacctgg tatgccagat
    13681 gagaatgaca ggagccatcc gcaagcagtt ggcggctttc ttagaaggct tctatgagat
    13741 cattccaaag cgcctcattt ccatcttcac tgagcaggag ttagagctgc ttatatcagg
    13801 actgcccacc attgacatcg atgatctgaa atccaacact gaataccaca agtaccagtc
    13861 caactctatt cagatccagt ggttctggag agcattgcgt tctttcgatc aagctgaccg
    13921 tgccaagttc ctccagtttg tcacgggtac ttccaaggta cccctgcaag gctttgctgc
    13981 cctcgaaggc atgaatggca ttcagaagtt tcagatccat cgagatgaca ggtccacaga
    14041 tcgcctgcct tcagctcaca caagctttaa tcagctggat ctgcctgcct atgagagctt
    14101 tgagaagctc cgccacatgc tactgttggc tatccaggag tgctctgaag gctttgggct
    14161 ggcctaataa gcggccgcga cccagctttc ttgtacaaag tggttgatat cggtaccagt
    14221 cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
    14281 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
    14341 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
    14401 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
    14461 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
    14521 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
    14581 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
    14641 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
    14701 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
    14761 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
    14821 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc
    14881 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    14941 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    15001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    15061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    15121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    15181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa
    15241 accagaaagt taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt
    15301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    15361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    15421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    15481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    15541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    15601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    15661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    15721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    15781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    15841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    15901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    15961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    16021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    16081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    16141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    16201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    16261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    16321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    16381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    16441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    16501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    16561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    16621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    16681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    16741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    16801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    16861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    16921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    16981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    17041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    17101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    17161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    17221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    17281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    17341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    17401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
    17461 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    17521 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    17581 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    17641 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    17701 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    17761 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    17821 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    17881 attaacgctt acaattt
//
