LOCUS       sequence-369464-       10737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CD3-epsilon/ZtSH2 pYtag biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR iRFP-ZtSH2
FEATURES             Location/Qualifiers
     source          1..10737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             301..345
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    630..747
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        900..1307
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1377..1386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1383..2330
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    3181..3769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3234..3254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3652..3663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3772..3788)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3773..3789)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3879..4112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4137..4155)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4138..4155)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4445..4464)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4678..4697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4797..4819
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4857..4875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4943..5047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5048..5116,5117..5908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5266..5285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6079..6667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6568..6587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6821..6838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6908..6928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6913..7242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7093..7228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8376..8441
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8571..8591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9016..9150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9053..9072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9107..9126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9318..9951
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9998..10123
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10620..10737,1..116)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
       61 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
      121 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
      181 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
      241 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
      301 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
      361 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      421 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      481 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      541 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      601 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
      661 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
      721 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
      781 tggatcgata agcttgatat cgaattcctg cagccccgat aaaataaaag attttattta
      841 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag
      901 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
      961 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     1021 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     1081 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     1141 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     1201 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     1261 acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggggg gatctggagc
     1321 tctcgagaat tctcacgcgt caagtggagc aaggcaggtg gacagtggat ccggtcgcca
     1381 ccatggctga aggatccgtc gccaggcagc ctgacctctt gacctgcgac gatgagccga
     1441 tccatatccc cggtgccatc caaccgcatg gactgctgct cgccctcgcc gccgacatga
     1501 cgatcgttgc cggcagcgac aaccttcccg aactcaccgg actggcgatc ggcgccctga
     1561 tcggccgctc tgcggccgat gtcttcgact cggagacgca caaccgtctg acgatcgcct
     1621 tggccgagcc cggggcggcc gtcggagcac cgatcactgt cggcttcacg atgcgaaagg
     1681 acgcaggctt catcggctcc tggcatcgcc atgatcagct catcttcctc gagctcgagc
     1741 ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg ccgcaccaac agcgccatcc
     1801 gccgcctgca ggccgccgaa accttggaaa gcgcctgcgc cgccgcggcg caagaggtgc
     1861 ggaagattac cggcttcgat cgggtgatga tctatcgctt cgcctccgac ttcagcggcg
     1921 aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa actaggcctg cactatcctg
     1981 cctcaaccgt gccggcgcag gcccgtcggc tctataccat caacccggta cggatcattc
     2041 ccgatatcaa ttatcggccg gtgccggtca ccccagacct caatccggtc accgggcggc
     2101 cgattgatct tagcttcgcc atcctgcgca gcgtctcgcc cgtccatctg gaattcatgc
     2161 gcaacatagg catgcacggc acgatgtcga tctcgatttt gcgcggcgag cgactgtggg
     2221 gattgatcgt ttgccatcac cgaacgccgt actacgtcga tctcgatggc cgccaagcct
     2281 gcgagctagt cgcccaggtt ctggcctggc agatcggcgt gatggaagag ggctccggct
     2341 ctccagaccc cgcggcgcac ctgcccttct tctacggcag catctcgcgt gccgaggccg
     2401 aggagcacct gaagctggcg ggcatggcgg acgggctctt cctgctgcgc cagtgcctgc
     2461 gctcgctggg cggctatgtg ctgtcgctcg tgcacgatgt gcgcttccac cactttccca
     2521 tcgagcgcca gctcaacggc acctacgcca ttgccggcgg caaagcgcac tgtggaccgg
     2581 cagagctctg cgagttctac tcgcgcgacc ccgacgggct gccctgcaac ctgcgcaagc
     2641 cgtgcaaccg gccgtcgggc ctcgagccgc agccgggggt cttcgactgc ctgcgagacg
     2701 ccatggtgcg tgactacgtg cgccagacgt ggaagctgga gggcgaggcc ctggagcagg
     2761 ccatcatcag ccaggccccg caggtggaga agctcattgc tacgacggcc cacgagcgga
     2821 tgccctggta ccacagcagc ctgacgcgtg aggaggccga gcgcaaactt tactctgggg
     2881 cgcagaccga cggcaagttc ctgctgaggc cgcggaagga gcagggcaca tacgccctgt
     2941 ccctcatcta tgggaagacg gtgtaccact acctcatcag ccaagacaag gcgggcaagt
     3001 actgcattcc cgagggcacc aagtttgaca cgctctggca gctggtggag tatctgaagc
     3061 tgaaggcgga cgggctcatc tactgcctga aggaggcctg ccccaacagc agtgcctgac
     3121 tgaggccgcg actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat
     3181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3541 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3721 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     3781 cgacctcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
     3841 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3901 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3961 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4021 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4081 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     4141 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     4201 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     4261 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     4321 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     4381 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     4441 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     4501 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     4561 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     4621 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     4681 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4741 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4801 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4861 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6721 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6781 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6841 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6901 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6961 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7081 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7141 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7201 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
     7261 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     7321 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     7381 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     7441 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     7501 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     7561 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
     7621 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
     7681 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
     7741 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
     7801 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
     7861 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
     7921 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
     7981 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
     8041 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
     8101 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     8161 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
     8221 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
     8281 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
     8341 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     8401 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     8461 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     8521 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     8581 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     8641 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     8701 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     8761 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     8821 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     8881 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     8941 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     9001 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     9181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9301 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     9361 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     9421 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     9481 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     9541 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     9601 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     9661 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     9721 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     9781 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9841 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9901 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9961 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10021 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10081 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10141 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10201 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10261 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10321 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10381 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10441 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
    10501 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10561 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10621 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10681 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaat
//
