LOCUS       sequence-369466-       13808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ErbB2 labeled with CD3-epsilon ITAMs for monitoring with 
            CD3-epsilon/ZtSH2 pYtag biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR ErbB2-CD3-epsilon-FusionRed
FEATURES             Location/Qualifiers
     source          1..13808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1464..1529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1659..1679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2104..2238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2195..2214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2406..3039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3086..3211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3708..3941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4126..4170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4455..4572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4725..5132
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             9272..9967
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    10077..10665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10130..10150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10548..10559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10668..10684)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10669..10685)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10775..11008
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11033..11051)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11034..11051)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11341..11360)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11574..11593)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11693..11715
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11753..11771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11839..11943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11944..12012,12013..12804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12162..12181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12975..13563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13464..13483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13717..13734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(join(13804..13808,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
      361 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      421 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      481 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
      541 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
      601 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
      661 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
      721 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
      781 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
      841 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
      901 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
      961 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     1021 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     1081 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     1141 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     1201 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     1261 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     1321 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     1381 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     1441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     1501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     1561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     1621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     1681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     1741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     1801 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     1861 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     1921 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     1981 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2041 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2101 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2221 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttcca
     2281 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     2341 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     2401 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     2461 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     2521 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     2581 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     2641 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     2701 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     2761 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     2821 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3061 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3121 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3181 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3241 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3301 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3361 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3421 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3481 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3541 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3601 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3661 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3721 cttgggttct tgggagcagc aggaagcact ataggcgcag cgtcaatgac gctgacggta
     3781 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3841 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3901 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3961 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4021 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4081 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4141 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4201 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4261 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4321 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4381 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca
     4441 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4561 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt
     4621 gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     4681 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
     4741 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     4801 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     4861 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     4921 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     4981 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5041 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5101 cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca
     5161 cgcgtcaagt ggagcaaggc aggtggacag tggatccatg gagctggcgg ccttgtgccg
     5221 ctgggggctc ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg
     5281 cacagacatg aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca
     5341 cctctaccag ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa
     5401 tgccagcctg tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca
     5461 caaccaagtg aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt
     5521 tgaggacaac tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc
     5581 tgtcacaggg gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat
     5641 cttgaaagga ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt
     5701 gtggaaggac atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg
     5761 ctctcgggcc tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag
     5821 ttctgaggat tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa
     5881 ggggccactg cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa
     5941 gcactctgac tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg
     6001 cccagccctg gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg
     6061 gtatacattc ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt
     6121 gggatcctgc accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac
     6181 acagcggtgt gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga
     6241 gcacttgcga gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa
     6301 gaagatcttt gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa
     6361 cactgccccg ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg
     6421 ttacctatac atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct
     6481 gcaagtaatc cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct
     6541 gggcatcagc tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat
     6601 ccaccataac acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa
     6661 cccgcaccaa gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg
     6721 cctggcctgc caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg
     6781 tgtcaactgc agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca
     6841 ggggctcccc agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca
     6901 gccccagaat ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc
     6961 ccactataag gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct
     7021 ctcctacatg cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat
     7081 caactgcacc cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc
     7141 cagccctctg acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg
     7201 ggtggtcttt gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg
     7261 gagactgctg caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa
     7321 ccaggcgcag atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc
     7381 tggcgctttt ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat
     7441 tccagtggcc atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt
     7501 agacgaagca tacgtgatgg ctggtgtggg ctccccatat gtctcccgcc ttctgggcat
     7561 ctgcctgaca tccacggtgc agctggtgac acagcttatg ccctatggct gcctcttaga
     7621 ccatgtccgg gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact ggtgtatgca
     7681 gattgccaag gggatgagct acctggagga tgtgcggctc gtacacaggg acttggccgc
     7741 tcggaacgtg ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg ggctggctcg
     7801 gctgctggac attgacgaga cagagtacca tgcagatggg ggcaaggtgc ccatcaagtg
     7861 gatggcgctg gagtccattc tccgccggcg gttcacccac cagagtgatg tgtggagtta
     7921 tggtgtgact gtgtgggagc tgatgacttt tggggccaaa ccttacgatg ggatcccagc
     7981 ccgggagatc cctgacctgc tggaaaaggg ggagcggctg ccccagcccc ccatctgcac
     8041 cattgatgtc tacatgatca tggtcaaatg ttggatgatt gactctgaat gtcggccaag
     8101 attccgggag ttggtgtctg aattctcccg catggccagg gacccccagc gctttgtggt
     8161 catccagaat gaggacttgg gcccagccag tcccttggac agcaccttct accgctcact
     8221 gctggaggac gatgacatgg gggacctggt ggatgctgag gagtatctgg taccccagca
     8281 gggcttcttc tgtccagacc ctgccccggg cgctgggggc atggtccacc acaggcaccg
     8341 cagctcatct accaggagtg gcggtgggga cctgacacta gggctggagc cctctgaaga
     8401 ggaggccccc aggtctccac tggcaccctc cgaaggggct ggctccgatg tatttgatgg
     8461 tgacctggga atgggggcag ccaaggggct gcaaagcctc cccacacatg accccagccc
     8521 tctacagcgg tacagtgagg accccacagt acccctgccc tctgagactg atggctacgt
     8581 tgcccccctg acctgcagcc cccagcctga atatgtgaac cagccagatg ttcggcccca
     8641 gcccccttcg ccccgagagg gccctctgcc tgctgcccga cctgctggtg ccactctgga
     8701 aaggcccaag actctctccc cagggaagaa tggggtcgtc aaagacgttt ttgcctttgg
     8761 gggtgccgtg gagaaccccg agtacttgac accccaggga ggagctgccc ctcagcccca
     8821 ccctcctcct gccttcagcc cagccttcga caacctctat tactgggacc aggacccacc
     8881 agagcggggg gctccaccca gcaccttcaa agggacacct acggcagaga acccagagta
     8941 cctgggtctg gacgtgccag tgggctctgg ttctccacct cccgtcccta atcccgatta
     9001 tgagcccatt cggaaaggtc aaagagattt gtatagtggc ctcaatcaaa gaagaatcgg
     9061 gtcaggttcc ggcccgccgc cagtcccaaa ccccgactat gaaccaatca ggaaaggcca
     9121 acgggatttg tactcaggat tgaatcagcg caggataggt tccggctccg ggccaccacc
     9181 cgtccctaac ccagattacg aacccatacg caagggacaa cgcgatcttt acagcggatt
     9241 gaaccaacga cgaataggat ctggttctgg catggtgagc gagctgatta aggagaacat
     9301 gcccatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     9361 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg tcgagggcgg
     9421 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt
     9481 catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg agggcttcac
     9541 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     9601 cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact tcccagccaa
     9661 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga cgatgtaccc
     9721 cgctgacggc ggcctggaag gcgcatgtga catggccctg aagctcgtgg gcgggggcca
     9781 cctgatctgc aaccttgaga ccacatacag atccaagaaa cccgctacga acctcaagat
     9841 gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg acgatgagac
     9901 ctacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg ctggtgatgg
     9961 aggtaaagcg gccacgcgtg cggccgcaac tcccacctgc aacatgcgtg actgactgag
    10021 gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc
    10081 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    10141 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    10201 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    10261 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    10321 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    10381 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    10441 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    10501 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    10561 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    10621 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
    10681 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
    10741 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    10801 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    10861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10981 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
    11041 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
    11101 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
    11161 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
    11221 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
    11281 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    11341 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    11401 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    11461 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    11521 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
    11581 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    11641 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    11701 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    11761 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    11821 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    11881 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    11941 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    12001 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    12061 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    12121 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    12181 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    12241 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    12301 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    12361 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    12421 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    12481 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    12541 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    12601 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    12661 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    12721 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    12781 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    12841 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    12901 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12961 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13021 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13081 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    13141 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    13201 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    13261 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    13321 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    13381 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    13441 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    13501 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    13561 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    13621 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    13681 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    13741 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    13801 tgtggaat
//
