LOCUS       sequence-36947-e        6454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Huff J, Czyz A, Landick R, Niederweis M
  TITLE     Taking phage integration to the next level as a genetic tool for 
            mycobacteria.
  JOURNAL   Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6.
  PUBMED    20692326
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2010-11-15"; volume: "468(1-2)"; pages: "8-19. Epub 2010 Aug 
            6"
COMMENT     Sequence Label: pML1357
FEATURES             Location/Qualifiers
     source          1..6454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1173..1206)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2658..3246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3147..3166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3400..3417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6019..6052)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacagaaag gaggttaata atgtcgaagg gcgaggagct gttcaccggc gtcgtcccga
       61 tcctggtcga gctggacggt gacgtcaacg gccacaagtt ctccgtctcc ggcgagggtg
      121 agggcgacgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggtaagctgc
      181 cggtcccgtg gccgaccctg gtcaccaccc tgacctacgg cgtccagtgc ttctcccgct
      241 acccggacca catgaagcgc cacgacttct tcaagtccgc catgccggag ggttacgtcc
      301 aggagcgcac catctccttc aaggacgacg gtaactacaa gacccgtgcc gaggtcaagt
      361 tcgagggcga caccctggtc aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gtaacatcct gggccacaag ctggagtaca actacaactc ccacaacgtc tacatcaccg
      481 cggacaagca gaagaacggc atcaaggcca acttcaagac ccgccacaac atcgaggacg
      541 gtggcgtcca gctagccgac cactaccagc agaacacccc gatcggcgac ggcccggtcc
      601 tgctgccgga caaccactac ctgtccaccc agtccgccct gtccaaggac ccgaacgaga
      661 agcgcgacca catggtcctg ctggagttcg tcaccgccgc cggcatcacc cacggcatgg
      721 acgagctgta caagtagatt taaataagct ttagctaatt aattggggac cctagaggtc
      781 ccctttttta ttttatcgat aaaaaaaaaa gccccgcaat cgcggggctt ttttttttac
      841 gcgtcgccga cctacacgta ttcgcagagt taagcggtca cccccgccgg tttgacccca
      901 attttgaccc caattgcatc ggggttcggg gcgattcgcc gatgtccgcc attgtgcgca
      961 accgggcgtg acctgcggat tcggtacccg ggcggatcca cgcggatgcc ttttgaatga
     1021 ctgggggtca aggggtcgca ggttcaaatc ctgtcagccc gacgcagatc aggggcggtt
     1081 ttccggagac gggaagccgc ccctttttct gtttgacccc aattttgacc ccaattgctc
     1141 cggcgtccgt ccgcgtccgt cgagggcgcg ccataacttc gtatagcata cattatacga
     1201 agttatgata tcaaaaaaaa gcccgccatt aggcgggctc aattgagcgg ccgctcaggt
     1261 cagcacggtc atgaagcgct cgttcaggat gcggtcgtgg tagaagatgg ccttgcccag
     1321 ctggtcggtg gtccaggtca ccggcttgtg gtccgggtag ttgtagtcgc cgccgcagaa
     1381 cacctcgttg cggttgcccg acgggtcgaa gaagtagatg gtcttgccgt gggtcaggcc
     1441 gtggcgggtc gggccgatgt cgatggaggt gtcggtcatc gagatcaggt cggcggcgcg
     1501 cagcaggtcc tcccaggtct ccaggtggaa cgacacgtgg tgcagccggc ccttctccgg
     1561 gtggtggatg aaggccacgt cgtgggcctt ggtggacagc gacaggaact gggccacgcg
     1621 ggtgccgttc tcgtccagca cctgctcggc caggtagaag cccagcacct tggtgaacag
     1681 gtcgtaggtg gccggcagct cgtcgccgta catcagggcg tggtcgaagc gcacggcggc
     1741 catgcccttc aggtcgcgcg gccaggcctc cgggttcacg tcgttcaggc cccacttgcc
     1801 ggtgtactcc ttgtcggcgt acagctcgaa gtggtggccc gacggggcct ggaagcgcac
     1861 gcggcggcca cacgagttca gctcgccggc cggcagctgc tccacggcac agccgtaggc
     1921 catcaggtcg cgctccagct ggcgcagggc gtcctcgtcc accaccttga agcccatgaa
     1981 gtccatgccc ggctcgtcgg cctcgcgcag caccagcgag aacttgtcca cctcggtcca
     2041 ggccttcagg tacacgcggc cctggtcgtc gcggtccatc tcgatcaggc ccagcagctc
     2101 gacgtagtgc tccagggcct tcgacatgtc cagcacgcgc agctggacgt ggcccggccg
     2161 catcacgccc ttgttcatta ttcctccttt ctagtacttc ggtcgacgtg tgcgcgtagc
     2221 cgctgcgcca gaacacccgc atcgcggtgt ccagtgcgac gtccggatcg aactccttgt
     2281 gacgggccat gtgccaattc tagttcgtcg agaaggtcga cggccagtgg aggacgtact
     2341 gctccgagac ctgctactgg accgacgccg tcgcgttccg gggtgagtac gagggcaggg
     2401 aaaccccgaa catgggacgg ctcaccgggt tccgagaatg ggagaccctg caccacggca
     2461 atccaataat tgtagaaata ttggtaactg tcagaccaag tttactcata tatactttag
     2521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3421 gccgacggta tcgggtaccc taagactgtc cgctgccgtc cgcgatccgc agtcgcgccg
     3481 acaacacgtc gacggcggcc cgcgcggtcg cgtcggacgt gtggccgtag atgtcccccg
     3541 tgaccgccac cgacgaatgc ccgaggatgt cggcgaccgc gcggatgtgg atgcccgcct
     3601 cgagcatcgc caccgccgcg gcgtgacgca gggtgtgcac gccgacgccc tcgaggccaa
     3661 cctgcgcggc ggcctgctcc accacccgca acaggttgcg cggatccacc gggcggccga
     3721 acgccgacgg gaacaccaac ccgtggtcct cccacacgtt ggccgcgcgc aggcgttcag
     3781 ctaactggtc ggcgcggtgc cgacgcagca tcgacaccat gtccggcgtc aacggcaccg
     3841 tccggcggga tcgttccgtc ttcggcgtcg tgaggcgcag gcgacgctcg acgcggccga
     3901 cggtggccac cacgcgcagc gacccggcgt cgaggtcgac ggcgtcccac cgaagggcca
     3961 acgcctcccc ggcgcgcagt ccggtcgccg cgatcagcaa cagggcgcgg tggtagcggg
     4021 atccggcggt ggcggccaac aggtcggcca actgcgcgtc gttgatccgc cggacttccg
     4081 ggcgcgacaa cccgggccgc ttcatggccg ccgcagggtt gcgcgccatc aggccgtcgc
     4141 gcaccgcgcc gtcgagcgcc tggcgcaaca cggtgtaggt cgaccggatc gtgctgtcgg
     4201 cgaccgcgcg caccggcgtc ccgtcgcggc ccgggcgggt ggcctgttga aggcgcagca
     4261 ccaacgcctc gacatcggat gtgcgcaggc ggtccaaccg gatcgcgccg aacggggcgg
     4321 gttcgacgta ttggcgcgac agtgtgccgt acaacgattt ggtgctgtcg gcgcggccgg
     4381 agaccaacag ggtggtggtg caccaccggc gcatccaatc ggccacggtc accgcggcgt
     4441 cacgcaccgg ggcgtcggcg tcgaggcggt cgatagcttc gcgcattttg gcccgtaccg
     4501 ccgtcttcgt cttgccgtag aacgatgccc ggcggcgttc accggtgacc gggtcgtcat
     4561 agagcaaccg ggcctcccag cgcccgtccg gccggtgccg gtatgtgcct tcgcggttgc
     4621 cgcgtcgccc cattgcggtg tcctctcggg ggtcgccggg tgcgtgggga tccacgccga
     4681 atagtgcccg gtcgggatag gtgtacggtc gcctccggtc acgtccgggc gtccgcgtac
     4741 gccgattgtg tcaccgcggc gatcatcatt ccgttaacga aatcaatcta aagtatatat
     4801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4861 tgtctatttc gttcatccat agttgcctga ctccccgacg tggccgacca gcccgtcatc
     4921 gtcaacgcct gatccgcggt gcggacaggc cgtgtcgcga ccggccgtgc ggaattaagc
     4981 cggcccgtac cctgtgaata gaggtccgct gtgacacaag aatccctgtt acttctcgac
     5041 cgtattgatt cggatgattc ctacgcgagc ctgcggaacg accaggaatt ctgggagccg
     5101 ctggcccgcc gagccctgga ggagctcggg ctgccggtgc cgccggtgct gcgggtgccc
     5161 ggcgagagca ccaaccccgt actggtcggc gagcccgacc cggtgatcaa gctgttcggc
     5221 gagcactggt gcggtccgga gagcctcgcg tcggagtcgg aggcgtacgc ggtcctggcg
     5281 gacgccccgg tgccggtgcc ccgcctcctc ggccgcggcg agctgcggcc cggcaccgga
     5341 gcctggccgt ggccctacct ggtgatgagc cggatgaccg gcaccacctg gcggtccgcg
     5401 atggacggca cgaccgaccg gaacgcgctg ctcgccctgg cccgcgaact cggccgggtg
     5461 ctcggccggc tgcacagggt gccgctgacc gggaacaccg tgctcacccc ccattccgag
     5521 gtcttcccgg aactgctgcg ggaacgccgc gcggcgaccg tcgaggacca ccgcgggtgg
     5581 ggctacctct cgccccggct gctggaccgc ctggaggact ggctgccgga cgtggacacg
     5641 ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc tgcacgggac caacatcttc
     5701 gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact tcaccgacgt ctatgcggga
     5761 gactcccgct acagcctggt gcaactgcat ctcaacgcct tccggggcga ccgcgagatc
     5821 ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg aggacttcgc ccgcgaactg
     5881 ctcgccttca ccttcctgca cgacttcgag gtgttcgagg agaccccgct ggatctctcc
     5941 ggcttcaccg atccggagga actggcgcag ttcctctggg ggccgccgga caccgccccc
     6001 ggcgcctgac gccccgggat aacttcgtat agcatacatt atacgaagtt atatgcattg
     6061 actaagctag atacctgaaa aaaaaaaagc gccgcagttg cggcgctttt ttttttctcg
     6121 actagtgttt aaactctaga aatattggat cgtcggcacc gtcacggccg tgggaggcgg
     6181 cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg agctctacgc
     6241 catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac
     6301 gagcgggaga acccacccgg ggtgggcgag tttgtcctgc gtgtgctcgg tcgagtaggc
     6361 tctgggagta cccgtgtgta cgaccagcac ggcatacatc atttcgacgc cgagagattc
     6421 gccgcccgaa atgagcacga tccgcatgct taat
//
