LOCUS       sequence-369471-       10397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Grb2 C-terminally labeled with TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR Grb2-TagBFP
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             386..430
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    715..832
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        985..1392
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2130..2828
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2926..3514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2979..2999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3397..3408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3517..3533)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3518..3534)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3624..3857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3882..3900)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3883..3900)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4190..4209)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4423..4442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4542..4564
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4602..4620)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4688..4792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4793..4861,4862..5653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5011..5030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5824..6412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6313..6332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6566..6583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6653..6673)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6658..6987
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6838..6973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8121..8186
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8316..8336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8761..8895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8798..8817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8852..8871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9063..9696
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9743..9868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10365..10397,1..201)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
       61 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
      121 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
      181 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
      241 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
      301 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
      361 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
      421 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
      481 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
      541 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
      601 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
      661 tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc acaattttaa
      721 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      781 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      841 ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat tcctgcagcc
      901 ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
      961 tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
     1021 ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa
     1081 acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc
     1141 gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc
     1201 agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct
     1261 gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt
     1321 cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact
     1381 gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtcaagt ggagcaaggc
     1441 aggtggacag tggatccatg gaagccatcg ccaaatatga cttcaaagct actgcagacg
     1501 acgagctgag cttcaaaagg ggggacatcc tcaaggtttt gaacgaagaa tgtgatcaga
     1561 actggtacaa ggcagagctt aatggaaaag acggcttcat tcccaagaac tacatagaaa
     1621 tgaaaccaca tccgtggttt tttggcaaaa tccccagagc caaggcagaa gaaatgctta
     1681 gcaaacagcg gcacgatggg gcctttctta tccgagagag tgagagcgct cctggggact
     1741 tctccctctc tgtcaagttt ggaaacgatg tgcagcactt caaggtgctc cgagatggag
     1801 ccgggaagta cttcctctgg gtggtgaagt tcaattcttt gaatgagctg gtggattatc
     1861 acagatctac atctgtctcc agaaaccagc agatattcct gcgggacata gaacaggtgc
     1921 cacagcagcc gacatacgtc caggccctct ttgactttga tccccaggag gatggagagc
     1981 tgggcttccg ccggggagat tttatccatg tcatggataa ctcagacccc aactggtgga
     2041 aaggagcttg ccacgggcag accggcatgt ttccccgcaa ttatgtcacc cccgtgaacc
     2101 ggaacgtcga tatcggatcc ggagctacca tgagcgagct gattaaggag aacatgcaca
     2161 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     2221 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     2281 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     2341 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     2401 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     2461 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     2521 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     2581 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     2641 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     2701 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     2761 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     2821 agcttaatgc ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct
     2881 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     2941 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3001 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3061 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3121 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3181 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3241 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3301 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3361 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3421 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3481 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     3541 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3841 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     3901 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     3961 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     4021 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     4081 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     4141 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     4201 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     4261 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     4321 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     4381 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     4441 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4501 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4561 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4621 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4681 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4741 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4981 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5101 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5341 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5401 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5641 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5701 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5761 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5821 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5881 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5941 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6001 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6061 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6481 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6541 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6601 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     6661 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6721 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6781 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6901 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6961 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     7021 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     7081 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     7141 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     7201 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     7261 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     7321 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     7381 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     7441 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     7501 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     7561 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     7621 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     7681 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     7741 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     7801 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     7861 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     7921 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     7981 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     8041 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     8101 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     8161 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     8221 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     8281 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     8341 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     8401 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     8461 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     8521 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     8581 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     8641 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8701 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8881 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     8941 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9001 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9061 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     9121 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9181 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9241 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9301 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9361 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9421 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9481 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9721 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9781 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9841 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     9901 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     9961 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    10021 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    10081 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    10141 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    10201 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    10261 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10321 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10381 gggttcttgg gagcagc
//
