LOCUS       sequence-369477-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human FGFR1 labeled with CD3-epsilon ITAMs for monitoring with 
            CD3-epsilon/ZtSH2 pYtag biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR FGFR1-CD3-epsilon-FusionRed
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1173..1238
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1368..1388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1813..1947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1850..1869)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1904..1923
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2115..2748
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2795..2920
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3417..3650
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3835..3879
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4164..4281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4434..4841
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             complement(5286..5303)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7676..8371
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    8481..9069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8534..8554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8952..8963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9072..9088)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9073..9089)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9179..9412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9437..9455)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9438..9455)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9745..9764)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9978..9997)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10097..10119
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10157..10175)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10243..10347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10348..10416,10417..11208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10566..10585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11379..11967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11868..11887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12121..12138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12208..12228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12213..12503,1..39)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12393..12503,1..25)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
       61 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
      121 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
      181 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
      241 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
      301 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
      361 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
      421 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
      481 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
      541 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
      601 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
      661 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
      721 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
      781 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
      841 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
      901 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
      961 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     1021 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     1081 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     1141 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     1201 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     1261 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     1321 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     1381 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     1441 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     1501 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     1561 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     1621 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     1681 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     1741 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1801 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1921 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     1981 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     2041 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     2101 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     2161 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     2221 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     2281 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     2341 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     2401 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     2461 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     2521 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     2581 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2641 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2701 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2761 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2821 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2881 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2941 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     3001 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     3061 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     3121 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     3181 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     3241 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     3301 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     3361 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     3421 gctttgttcc ttgggttctt gggagcagca ggaagcacta taggcgcagc gtcaatgacg
     3481 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     3541 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3601 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3661 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3721 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3781 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3841 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3901 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3961 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     4021 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     4081 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     4141 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     4201 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4261 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
     4321 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc
     4381 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc
     4441 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
     4501 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
     4561 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     4621 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     4681 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     4741 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     4801 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga
     4861 gaattctcac gcgtcaagtg gagcaaggca ggtggacagt ggatccatgt ggagctggaa
     4921 gtgcctcctc ttctgggctg tgctggtcac agccacactc tgcaccgcta ggccgtcccc
     4981 gaccttgcct gaacaagccc agccctgggg agcccctgtg gaagtggagt ccttcctggt
     5041 ccaccccggt gacctgctgc agcttcgctg tcggctgcgg gacgatgtgc agagcatcaa
     5101 ctggctgcgg gacggggtgc agctggcgga aagcaaccgc acccgcatca caggggagga
     5161 ggtggaggtg caggactccg tgcccgcaga ctccggcctc tatgcttgcg taaccagcag
     5221 cccctcgggc agtgacacca cctacttctc cgtcaatgtt tcagatgctc tcccctcctc
     5281 ggaggatgat gatgatgatg atgactcctc ttcagaggag aaagaaacag ataacaccaa
     5341 accaaaccgt atgcccgtag ctccatattg gacatcccca gaaaagatgg aaaagaaatt
     5401 gcatgcagtg ccggctgcca agacagtgaa gttcaaatgc ccttccagtg ggaccccaaa
     5461 ccccacactg cgctggttga aaaatggcaa agaattcaaa cctgaccaca gaattggagg
     5521 ctacaaggtc cgttatgcca cctggagcat cataatggac tctgtggtgc cctctgacaa
     5581 gggcaactac acctgcattg tggagaatga gtacggcagc atcaaccaca cataccagct
     5641 ggatgtcgtg gagcggtccc ctcaccggcc catcctgcaa gcagggttgc ccgccaacaa
     5701 aacagtggcc ctgggtagca acgtggagtt catgtgtaag gtgtacagtg acccgcagcc
     5761 gcacatccag tggctaaagc acatcgaggt gaatgggagc aagattggcc cagacaacct
     5821 gccttatgtc cagatcttga agactgctgg agttaatacc accgacaaag agatggaggt
     5881 gcttcactta agaaatgtct cctttgagga cgcaggggag tatacgtgct tggcgggtaa
     5941 ctctatcgga ctctcccatc actctgcatg gttgaccgtt ctggaagccc tggaagagag
     6001 gccggcagtg atgacctcgc ccctgtacct ggagatcatc atctattgca caggggcctt
     6061 cctcatctcc tgcatggtgg ggtcggtcat cgtctacaag atgaagagtg gtaccaagaa
     6121 gagtgacttc cacagccaga tggctgtgca caagctggcc aagagcatcc ctctgcgcag
     6181 acaggtgtct gctgactcca gtgcatccat gaactctggg gttcttctgg ttcggccatc
     6241 acggctctcc tccagtggga ctcccatgct agcaggggtc tctgagtatg agcttcccga
     6301 agaccctcgc tgggagctgc ctcgggacag actggtctta ggcaaacccc tgggagaggg
     6361 ctgctttggg caggtggtgt tggcagaggc tatcgggctg gacaaggaca aacccaaccg
     6421 tgtgaccaaa gtggctgtga agatgttgaa gtcggacgca acagagaaag acttgtcaga
     6481 cctgatctca gaaatggaga tgatgaagat gatcgggaag cataagaata tcatcaacct
     6541 gctgggggcc tgcacgcagg atggtccctt gtatgtcatc gtggagtatg cctccaaggg
     6601 caacctgcgg gagtacctgc aggcccggag gcccccaggg ctggaatact gctacaaccc
     6661 cagccacaac ccagaggagc agctctcctc caaggacctg gtgtcctgcg cctaccaggt
     6721 ggcccgaggc atggagtatc tggcctccaa gaagtgcata caccgagacc tggcagccag
     6781 gaatgtcctg gtgacagagg acaatgtgat gaagatagca gactttggcc tcgcacggga
     6841 cattcaccac atcgactact ataaaaagac aaccaacggc cgactgcctg tgaagtggat
     6901 ggcacccgag gcattatttg accggatcta cacccaccag agtgatgtgt ggtctttcgg
     6961 ggtgctcctg tgggagatct tcactctggg cggctcccca taccccggtg tgcctgtgga
     7021 ggaacttttc aagctgctga aggagggtca ccgcatggac aagcccagta actgcaccaa
     7081 cgagctgtac atgatgatgc gggactgctg gcatgcagtg ccctcacaga gacccacctt
     7141 caagcagctg gtggaagacc tggaccgcat cgtggccttg acctccaacc aggagtacct
     7201 ggacctgtcc atgcccctgg accagtactc ccccagcttt cccgacaccc ggagctctac
     7261 gtgctcctca ggggaggatt ccgtcttctc tcatgagccg ctgcccgagg agccctgcct
     7321 gccccgacac ccagcccagc ttgccaatgg cggactcaaa cgccgcggct ctggttctcc
     7381 acctcccgtc cctaatcccg attatgagcc cattcggaaa ggtcaaagag atttgtatag
     7441 tggcctcaat caaagaagaa tcgggtcagg ttccggcccg ccgccagtcc caaaccccga
     7501 ctatgaacca atcaggaaag gccaacggga tttgtactca ggattgaatc agcgcaggat
     7561 aggttccggc tccgggccac cacccgtccc taacccagat tacgaaccca tacgcaaggg
     7621 acaacgcgat ctttacagcg gattgaacca acgacgaata ggatctggtt ctggcatggt
     7681 gagcgagctg attaaggaga acatgcccat gaagctgtac atggagggca ccgtgaacaa
     7741 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     7801 gagaatcaag gtcgtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
     7861 cttcatgtac ggcagcagaa ccttcatcaa gcaccctccg ggcatccccg acttctttaa
     7921 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     7981 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaaggt
     8041 tagaggggtg aacttcccag ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     8101 ggcctccacc gagacgatgt accccgctga cggcggcctg gaaggcgcat gtgacatggc
     8161 cctgaagctc gtgggcgggg gccacctgat ctgcaacctt gagaccacat acagatccaa
     8221 gaaacccgct acgaacctca agatgcccgg cgtctacaac gtggaccaca gactggaaag
     8281 aatcaaggag gccgacgatg agacctacgt cgagcagcac gaggtggctg tggccagata
     8341 ctctactggt ggcgctggtg atggaggtaa agcggccacg cgtgcggccg caactcccac
     8401 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc
     8461 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     8521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9001 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9061 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     9121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9181 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     9241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     9421 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     9481 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     9541 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     9601 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     9661 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     9721 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     9781 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     9841 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     9901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     9961 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
    10021 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    10081 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    10141 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
    10201 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    10261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    10381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    10441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    10501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    10561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    10621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    11041 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    11101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    11161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    11221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    11281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    11341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    12001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    12061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    12121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    12181 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    12241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    12301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    12361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    12421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    12481 cggcctctga gctattccag aag
//
