LOCUS       sequence-369483-        5075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage P1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_P1
FEATURES             Location/Qualifiers
     source          1..5075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     242..261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     495..512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    527..667
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(593..612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     753..775
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(931..1809)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1836..2120
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2043..2062
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3659..3686
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3705..3796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3797..3865,3866..4657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4015..4034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4828..5075,1..341)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
       61 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      361 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      421 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      481 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
      541 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      601 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
      661 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      781 accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca
      841 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
      901 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
      961 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
     1021 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
     1081 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
     1141 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
     1201 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
     1261 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
     1321 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
     1381 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
     1441 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
     1501 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
     1561 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
     1621 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
     1681 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
     1741 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
     1801 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
     1861 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
     1921 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
     1981 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
     2041 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
     2101 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accatggcca
     2161 gaagttgcgt aacggaccca cgttggcgcg agcttgtggc gctatatcgt tatgactgga
     2221 ttgcggccgc tgatgtgttg tttgggaaga caccaacctg gcagcaggat gagatcattg
     2281 agtccacgca gcaggacggc agttggacaa gtgtgacctc cggccatggt actggtaaat
     2341 cggatatgac gagtatcatt gcaatactct tcatcatgtt tttccccggc gctcgcgtca
     2401 ttctggtcgc taacaaaaga cagcaagtcc ttgatggtat tttcaaatac ataaagagca
     2461 attgggctac tgctgttagc agattcccgt ggttgtcgaa gtatttcatt cttacagaaa
     2521 cgtctttttt tgaggtgact ggcaagggtg tttggacaat attgataaag tcctgtcgtc
     2581 ccggaaatga ggaggcgttg gctggtgaac acgccgatca tctcttgtat atcatcgacg
     2641 aagcgtcggg tgtgagtgat aaagcattca gtgtgataac aggtgcgctg accggtaagg
     2701 ataaccgtat tctgcttctt tcccagccta cgcgaccttc aggctatttc tacgattcac
     2761 accacagact agctattcgc ccgggaaatc ctgatggatt gtttactgcg ataatactga
     2821 atagtgaaga atctccgctt gtagatgcaa aatttatacg agcaaaactt gcggagtatg
     2881 gcggtcgtga taaccccatg tacatgatca aagtacgtgg tgaatttccc aaatctcaag
     2941 atggctttct tcttggtcgt gatgaggttg agcgggcgac gcggcgaaag gtcaagattg
     3001 ccaaaggatg gggctgggtt gcatgtgttg acgttgctgg tggcacagga cgagataagt
     3061 ccgttattaa tatcatgatg gtgtccggcc agcgaaataa acgccgtgta atcaactatc
     3121 gtatgctgga atacacagac gttacagaaa cgcagttagc cgccaagatt ttcgcagaat
     3181 gtaacccaga acggttcccg aacataacca tagctattga tggcgatggc ttggggaaat
     3241 cgacggctga tctaatgtac gaacgctatg gcattaccgt ccagcgtatc cgctggggta
     3301 aaaagatgca cagccgtgaa gataaaagcc tttatttcga tatgcgcgct ttcgcgaata
     3361 ttcaggcggc agaagctgta aaatcagggc gtatgaggct tgataagggg gctgcgacta
     3421 tagaggaagc atcaaagata ccggtaggga taaattccgc aggtcaatgg aaggtgatgt
     3481 caaaggaaga tatgaagaaa aaactcaacc tgcactcacc ggaccattgg gatacatatt
     3541 gtttcgctat gttggcgaac tatgttcccc aagatgaagt gcttagcgtc gaagacgaag
     3601 cgcaggttga tgaagctctg gcatggctta atgaataatg ccaggcatca aattaagcag
     3661 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     3721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3781 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3841 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3901 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3961 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4021 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4081 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4141 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4201 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4261 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4321 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4381 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4441 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4501 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4561 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4621 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4681 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4741 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4801 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4861 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4921 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4981 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5041 tctgctaatc ctgttaccag tggctgctgc cagtg
//
