LOCUS       sequence-369485-        5060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from Escherichia coli M885 prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_prophage_Escherichia_coli_2
FEATURES             Location/Qualifiers
     source          1..5060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     512..529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    544..684
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(610..629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     770..792
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(948..1826)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1853..2137
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2060..2079
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3661..3688
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3707..3798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3799..3867,3868..4659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4017..4036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4830..5060,1..358)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
       61 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      541 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      601 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      661 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      721 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      781 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc
      841 gcgaaggcga agcggcatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa
      901 accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg
      961 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg
     1021 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg
     1081 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg
     1141 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc
     1201 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg
     1261 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac
     1321 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc
     1381 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa
     1441 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag
     1501 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg
     1561 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca
     1621 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg
     1681 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg
     1741 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc
     1801 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc
     1861 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     1921 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     1981 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     2041 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     2101 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgttttttgg gctaacagga
     2161 ggaattaacc atgaccgaca ccgcgctgag cccggaggaa caactgatcg aggacattgc
     2221 gggcttcacc cacgatccgc tgggttacgc gctgtatgcg tttccgtggg gcgaggaagg
     2281 taccgaactg gcgcatgcga ccggtccgcg tcaatggcag gcggacgcgt tccgtgagat
     2341 ccgtgatcac ctgcaaaacc cggaaacccg ttatcaaccg ctgatgctgg cgcgtgcgag
     2401 cggtcatggt attggcaaga gcgcgtttat cagcatgctg attaactggg gtatgagcac
     2461 ctgcgaggac tgcaaagtgg ttgtgaccgc gaacaccgat aaccagctgc gtaccaagac
     2521 ctggccggaa atcattaaat ggagcaacct ggcgatcacc aaggactggt tcacctgcac
     2581 cgcgaccgcg atgtatagca acgacccggg ccacgataaa cgttggcgtg cggatgcgat
     2641 tccgtggagc gagcacaaca ccgaagcgtt cgcgggtctg cacaacgagc gtaagcgtat
     2701 cattgttgtg tttgacgaag cgagcaacat cgcggatctg gtttgggaag tggcggaagg
     2761 tgcgctgacc gacgaggata ccgaaatcat ttgggttgcg ttcggcaacc cgacccgtaa
     2821 caccggtcgt ttccgtgagt gctttcgtaa gtacaaacac cgttggaaaa ccgcgcaaat
     2881 cgacagccgt accgttgagg gcaccaacaa gcagcaactg cagaaatggg tggacgatta
     2941 tggcgaagac agcgattttg ttaagatccg tgtgcgtggt attttcccgg acgcgagcga
     3001 gctgcaattt atcccgaccg gtctgaccga tgaagcgatg aaacgtgttg ttaccgcggc
     3061 gcaggttgcg catgcgccgg ttatcattgg cgtggacccg gcgtacagcg gtgttgacga
     3121 tgcggtgatt tatctgcgtc aaggcctgca cagcaaggtg ctgtggaccg gtaacaaaac
     3181 caccgacgat ctgatcatgg cgaagcgtat tgcggacttc gaggatcaat accaggcgga
     3241 cgcggttttc atcgattttg gctatggtac cggcctgaaa agcattggtg acggctgggg
     3301 tcgtacctgg caactggtgc cgtttggtgg cgcgagcacc gatccgcaga tgctgaacaa
     3361 gcgtggcgaa atgtttaaca gctgcaaaac ctggctgcgt ctgggtggca tgctggacga
     3421 tcaagagacc gcggatgatc tgagcgcggc ggaatacaag gttcgtgtgg acggcaaaat
     3481 cgttattgag ccgaaggaag atatcaaaga gcgtctgggt cgtagcccgg gcaagggtga
     3541 tgcgctgctg ctgaccttcg cgtttccggt ggcgaagaaa accaacgatc cgcgtcagca
     3601 acagggtcgt gcgattaccg actatgatcc gtttgaataa tgccaggcat caaattaagc
     3661 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     3721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3781 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3841 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3901 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3961 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4021 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     4081 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4141 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4201 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4261 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4321 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4381 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4441 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4501 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4561 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4621 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4681 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4741 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4801 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4861 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4921 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4981 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5041 gctctgctaa tcctgttacc
//
