LOCUS       sequence-369488-        5156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage ZL19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_ZL19
FEATURES             Location/Qualifiers
     source          1..5156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..67
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    82..222
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(148..167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     308..330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(486..1364)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1391..1675
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1598..1617
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3295..3322
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3341..3432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3433..3501,3502..4293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3651..3670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4464..5052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4953..4972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
       61 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      121 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      181 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
      241 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      301 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      361 cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac
      421 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
      481 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
      541 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
      601 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      661 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
      721 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
      781 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
      841 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
      901 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
      961 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     1021 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     1081 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     1141 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     1201 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     1261 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     1321 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     1381 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     1441 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     1501 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     1561 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     1621 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     1681 ttttttgggc taacaggagg aattaaccat gtctttagta tgggaagaaa tgaccattca
     1741 ggaaaagatg gccgtcaagg cgatatcaga acacagcttc gagggctttc ttcgctgctg
     1801 gttccaaatc acacaaggcg agcgatacat ccctaactgg caccacaaat atctatgccg
     1861 aattatcgac gagattatag caggggagcg aaaggacacc atcatcaaca tagcgccggg
     1921 tagtggaaag acggaaatcg cttcaattca ctttccggcc tactctatgg ttaagttaaa
     1981 gaaggttcgc aaccttaaca tctcttttgc cgactcactg gttaagcgca actccaagcg
     2041 cgtacgtgac ctcatcaagt ctcaagaatt tcagtcactc tggccatgta agtttggcac
     2101 ttgcaaagac gatgagcttc aggttcttga tgagagtggc aaggtgagat ttgagtcaat
     2161 ctcgaaagcg gctggtggtc agattactgg tgctcgaggt gggtacataa ctgaaacata
     2221 tagcggcgcg gttttgctgg atgattttga caagcccgcc gatatgctat cagcagtgta
     2281 ccgctcgaat aatcacgtaa tgctaaagaa cacgattcgc tcacgtcgcg ctagctctgt
     2341 aaaaggaaag gcgacaccaa taatttcgat acagcagcga ctgcacgtca atgactcaac
     2401 gtatttcatg atggaaggcg gtatgggtat cgactttgac ttgattaaga tacctgcgct
     2461 agttactgag gattacatag attcgcttcc tgactggatt aaagaacagt ttgcggaaga
     2521 cgtattaagc agtgagtgca ttgagcgaga tggcgtaaag tactacagct actttcctaa
     2581 aaaggagtca gtaaacgacc ttgttgcgat gtgggatagt gacccgtaca ctttccttag
     2641 tcaatatcag caggaacctg tagcactagg tggaaacctc attaatgtgg attggttcca
     2701 gcgaatcagc gatactttca gacctccagc taagtatgac tatagattca tcacatgcga
     2761 taccgcgatg actacaaaga gttatagtga cttctctgtg cttcaactgt ggggatataa
     2821 ggatgctaag atttacctta tagaccagcg tcgaggcaaa tgggaggcgc ctgagttgga
     2881 ggcggaacta ctggacttcg agaggaaatc aagggcaacc agtcaggtag atggcatcct
     2941 acgaaaaatc atcatcgaga agaaatcatc cggaatcgga cttattcagt cggcaggcag
     3001 agtgatgaga acgccgatag agccatacgt acctgataac gacaagctaa ccagggtaat
     3061 gagcgcactg ccacagatta aggctggcaa tgtagttctt ccagaatcag cgccgtggtt
     3121 aagcggattg ctcacggaga ttgcagcttt tacggcagat gatagccaca aacacgatga
     3181 ccaaattgac tgtctaacaa tggcagtaaa ccttgttctc aatatcgcag atgatcctaa
     3241 gtccagatta atgcgcatag cgggaattaa ataatgccag gcatcaaatt aagcagaagg
     3301 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     3361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3421 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3541 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3601 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3661 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3721 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3781 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3841 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3901 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3961 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4021 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4081 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4141 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4201 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4261 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattac
//
