LOCUS       sequence-36949-e        6594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Huff J, Czyz A, Landick R, Niederweis M
  TITLE     Taking phage integration to the next level as a genetic tool for 
            mycobacteria.
  JOURNAL   Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6.
  PUBMED    20692326
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2010-11-15"; volume: "468(1-2)"; pages: "8-19. Epub 2010 Aug 
            6"
COMMENT     Sequence Label: pML1361
FEATURES             Location/Qualifiers
     source          1..6594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1379..1412)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2864..3452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3353..3372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3606..3623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6159..6192)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacagaaag gaggttaata atgtcgaagg gcgaggagct gttcaccggc gtcgtcccga
       61 tcctggtcga gctggacggt gacgtcaacg gccacaagtt ctccgtctcc ggcgagggtg
      121 agggcgacgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggtaagctgc
      181 cggtcccgtg gccgaccctg gtcaccaccc tgacctacgg cgtccagtgc ttctcccgct
      241 acccggacca catgaagcgc cacgacttct tcaagtccgc catgccggag ggttacgtcc
      301 aggagcgcac catctccttc aaggacgacg gtaactacaa gacccgtgcc gaggtcaagt
      361 tcgagggcga caccctggtc aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gtaacatcct gggccacaag ctggagtaca actacaactc ccacaacgtc tacatcaccg
      481 cggacaagca gaagaacggc atcaaggcca acttcaagac ccgccacaac atcgaggacg
      541 gtggcgtcca gctagccgac cactaccagc agaacacccc gatcggcgac ggcccggtcc
      601 tgctgccgga caaccactac ctgtccaccc agtccgccct gtccaaggac ccgaacgaga
      661 agcgcgacca catggtcctg ctggagttcg tcaccgccgc cggcatcacc cacggcatgg
      721 acgagctgta caagtagatt taaataagct ttagctaatt aattggggac cctagaggtc
      781 ccctttttta ttttatcgat aaaaaaaaaa gccccgcaat cgcggggctt ttttttttac
      841 gcgtccacca atttctcgtt acgtcgaaag tccgcgtttc cgcacacggg ggtgccgcat
      901 atacaggggc gctgtgcaca ggggtgtggg cagcagcagg gggctagggc acaggggtag
      961 gtgagggtgt gggccgcgag ggcctcagcc ccccgctcac gcgcaaccgc cgctcggtat
     1021 tcagcagaca cccacatgtc cgtgttgtca ctgacaacac ggctccaggt tttcccaggt
     1081 cgctacaggt ctaaaaaggt cggaacagaa ccacacgggt gttttttcgc aggtaaacgc
     1141 ccatttcccc acgatcgaag gggttcgaat ccccttagct ccacccaaaa ccgcaggtca
     1201 gcgaatcgcc cagaatctga cagcaccgat gacatcacaa cggatagaat ccgggtatgg
     1261 catcagtgcg tgaacgggtc cgcaaagacg gaaccaccgc ctacctggtc tcctaccggt
     1321 tcggcggcag aggaagcgca caaggcgcac tcaccttcga caatcgcaaa ggcgcgccat
     1381 aacttcgtat agcatacatt atacgaagtt atgatatcaa aaaaaagccc gccattaggc
     1441 gggctcaatt gagcggccgc tcaggtcagc acggtcatga agcgctcgtt caggatgcgg
     1501 tcgtggtaga agatggcctt gcccagctgg tcggtggtcc aggtcaccgg cttgtggtcc
     1561 gggtagttgt agtcgccgcc gcagaacacc tcgttgcggt tgcccgacgg gtcgaagaag
     1621 tagatggtct tgccgtgggt caggccgtgg cgggtcgggc cgatgtcgat ggaggtgtcg
     1681 gtcatcgaga tcaggtcggc ggcgcgcagc aggtcctccc aggtctccag gtggaacgac
     1741 acgtggtgca gccggccctt ctccgggtgg tggatgaagg ccacgtcgtg ggccttggtg
     1801 gacagcgaca ggaactgggc cacgcgggtg ccgttctcgt ccagcacctg ctcggccagg
     1861 tagaagccca gcaccttggt gaacaggtcg taggtggccg gcagctcgtc gccgtacatc
     1921 agggcgtggt cgaagcgcac ggcggccatg cccttcaggt cgcgcggcca ggcctccggg
     1981 ttcacgtcgt tcaggcccca cttgccggtg tactccttgt cggcgtacag ctcgaagtgg
     2041 tggcccgacg gggcctggaa gcgcacgcgg cggccacacg agttcagctc gccggccggc
     2101 agctgctcca cggcacagcc gtaggccatc aggtcgcgct ccagctggcg cagggcgtcc
     2161 tcgtccacca ccttgaagcc catgaagtcc atgcccggct cgtcggcctc gcgcagcacc
     2221 agcgagaact tgtccacctc ggtccaggcc ttcaggtaca cgcggccctg gtcgtcgcgg
     2281 tccatctcga tcaggcccag cagctcgacg tagtgctcca gggccttcga catgtccagc
     2341 acgcgcagct ggacgtggcc cggccgcatc acgcccttgt tcattattcc tcctttctag
     2401 tacttcggtc gacgtgtgcg cgtagccgct gcgccagaac acccgcatcg cggtgtccag
     2461 tgcgacgtcc ggatcgaact ccttgtgacg ggccatgtgc caattctagt tcgtcgagaa
     2521 ggtcgacggc cagtggagga cgtactgctc cgagacctgc tactggaccg acgccgtcgc
     2581 gttccggggt gagtacgagg gcagggaaac cccgaacatg ggacggctca ccgggttccg
     2641 agaatgggag accctgcacc acggcaatcc aataattgta gaaatattgg taactgtcag
     2701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3601 agcgcagcga gtcagtgagc gaggaagccg acggtatcgg gtaccctgcg gaacctggac
     3661 tatgaccgag tgaggtccgc aaacacaaaa aggcgcccta ccagggatct agatcccctg
     3721 gtagggcgca tctgggtctt aaaagtcccc caacaatccg tccataaact ccgccgccac
     3781 ccgcgaactc gtccgatcca catccgtgta cgtgtccacc gtgatctgaa tcgactcatg
     3841 acccagctgg cgagacacaa tcgtcaccgg tgtcccgccc gttagctgcc acgacgcgta
     3901 cgtgtgccgc agatcgtgcg gagtaggccg cggaaccaga ccagccttct ccacagccgg
     3961 attccacacc ctacgcagaa accccggata cctgaccggt ccaccatcgg tattgacgaa
     4021 aacaaactcg ttcgacaagt ccagccgctc caacaacctg gccggcacat ccaccgtgcg
     4081 gcgggaccgt ttcgtcttcg gcggccccaa cacatacccg gccgacgagt acttccacgc
     4141 ctgccgcacc ctgatcgtgg acgtctccaa atccacatgc ctgggctgca gcgccgatac
     4201 ctcaccccac cgcaaaccgg tcgacaccat gaactgaacc atcagcttcc agtgaggtgt
     4261 caccgcgtcg cggagccggt cgaactcggc gtgggtgagc atgcggatct cgtcgtcgtc
     4321 ctcagcgttc ccacggggca ggcggcggcc cgacgcaggg ttggtggaca agtatcgggg
     4381 gacggcggcg ttcagtgccc ccgataggaa cccgtatttg ttgcggaggg ttttcggggc
     4441 gtgcccgttg ccgtcgcggc cgccggtggt ttccatgacc ttcacccagc gggcgatgtc
     4501 ctcttcggag agcttcgaca aggggatgtc gccgaggttg ggttcgatgt cgttggcgag
     4561 gtactgctcg tatttgtcga tcgtgtactg ctcgacgccg gtgaggtggt cgatgtggtg
     4621 ccggatccat tcggcgacgg tcagctcgga cttggttcct cgcggtgcgg ggttgatgcc
     4681 gtgcatctcc agggcgcgtg cagcaccgtg ggcgtcgacg gcggcggcga aggcgtctgc
     4741 tgctttgcga ttgtcgaagg tgagtgcgcc ttgtgcgctt cctctgccgc cgaaccggta
     4801 ggagaccagg taggcggtgg ttccgtcttt gcggacccgt tcacgcactg atgccatacc
     4861 cggattctat ccgttgtgat gtcatcggtg ctgtcagatt ctgggcgatt cggttaacga
     4921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgacg
     5041 tggccgacca gcccgtcatc gtcaacgcct gatccgcggt gcggacaggc cgtgtcgcga
     5101 ccggccgtgc ggaattaagc cggcccgtac cctgtgaata gaggtccgct gtgacacaag
     5161 aatccctgtt acttctcgac cgtattgatt cggatgattc ctacgcgagc ctgcggaacg
     5221 accaggaatt ctgggagccg ctggcccgcc gagccctgga ggagctcggg ctgccggtgc
     5281 cgccggtgct gcgggtgccc ggcgagagca ccaaccccgt actggtcggc gagcccgacc
     5341 cggtgatcaa gctgttcggc gagcactggt gcggtccgga gagcctcgcg tcggagtcgg
     5401 aggcgtacgc ggtcctggcg gacgccccgg tgccggtgcc ccgcctcctc ggccgcggcg
     5461 agctgcggcc cggcaccgga gcctggccgt ggccctacct ggtgatgagc cggatgaccg
     5521 gcaccacctg gcggtccgcg atggacggca cgaccgaccg gaacgcgctg ctcgccctgg
     5581 cccgcgaact cggccgggtg ctcggccggc tgcacagggt gccgctgacc gggaacaccg
     5641 tgctcacccc ccattccgag gtcttcccgg aactgctgcg ggaacgccgc gcggcgaccg
     5701 tcgaggacca ccgcgggtgg ggctacctct cgccccggct gctggaccgc ctggaggact
     5761 ggctgccgga cgtggacacg ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc
     5821 tgcacgggac caacatcttc gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact
     5881 tcaccgacgt ctatgcggga gactcccgct acagcctggt gcaactgcat ctcaacgcct
     5941 tccggggcga ccgcgagatc ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg
     6001 aggacttcgc ccgcgaactg ctcgccttca ccttcctgca cgacttcgag gtgttcgagg
     6061 agaccccgct ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg
     6121 ggccgccgga caccgccccc ggcgcctgac gccccgggat aacttcgtat agcatacatt
     6181 atacgaagtt atatgcattg actaagctag atacctgaaa aaaaaaaagc gccgcagttg
     6241 cggcgctttt ttttttctcg actagtgttt aaactctaga aatattggat cgtcggcacc
     6301 gtcacggccg tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg
     6361 ctgcgcagtg agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg
     6421 gtgtgagaag ggtctctgac gagcgggaga acccacccgg ggtgggcgag tttgtcctgc
     6481 gtgtgctcgg tcgagtaggc tctgggagta cccgtgtgta cgaccagcac ggcatacatc
     6541 atttcgacgc cgagagattc gccgcccgaa atgagcacga tccgcatgct taat
//
