LOCUS       sequence-369491-        5183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage N4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_N4
FEATURES             Location/Qualifiers
     source          1..5183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    322..462
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(388..407)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     548..570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(726..1604)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1631..1915
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1838..1857
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3562..3589
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3608..3699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3700..3768,3769..4560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3918..3937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4731..5183,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      361 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      421 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
      481 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      541 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      601 cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac
      661 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
      721 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
      781 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
      841 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      901 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
      961 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     1021 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     1081 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     1141 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     1201 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     1261 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     1321 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     1381 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     1441 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     1501 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     1561 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     1621 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     1681 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     1741 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     1801 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     1861 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     1921 ttttttgggc taacaggagg aattaaccat gagcgagctg atcaaacagg agctggatga
     1981 atggctggat caagtggact acagcgttct gaacaccccg acctatatcc cgaccgaatt
     2041 tgcgctgacc ttcgcgaact ttattaaact ggtgaacggt aaagagggtg aaagcaacaa
     2101 gaccccgccg gttcacctga agatgctgga caaaatcacc agcaagaacc agtacattgc
     2161 gaacctgtgc tttcgtggcg cggcgaaaac caccctgttc atggagtatt tcaccctgtt
     2221 tctggcggtt ttcggtcacc tgccgagcct gggcaaggtg gaaggcatga tttacgttag
     2281 cgatagcatg gacaacggtg tgaaaagcgc gcgtaagaac atcgagttcc gttacaacaa
     2341 cagcccgttt ctgcagcaat ggattccgaa agcgaccttc accgataact atctggagtt
     2401 tgtgaacgcg gaaggtcacc gtctgggcgt taaaatgttt ggtgcgaaga ccggtctgcg
     2461 tggcaccaaa atcttcggca agcgtccggt gctgtgcgtt ctggacgatc tggttagcga
     2521 cgatgacgcg cgtagccgta ccagcatgga ggcgattaaa gataccgtgt ataagggtgt
     2581 taaccacgcg ctggacccga cccgtcgtaa agtgatcttc aacggcaccc cgtttaacaa
     2641 ggaagatatc ctgattgagg cggtggaaag cggtgcgtgg gacgtgaacg tttggccggt
     2701 ttgcgagaag ttcccgtgca cccgtgagga atttcagggc gcgtgggaag accgttttag
     2761 ctacgattat attaacgacc agtaccaaat ggcgctgaag accggtaaac tggcgagctt
     2821 ctatcaagag ctgatgctgc gtatcagcag cgaggatgaa cgtctgatcc aggacagcga
     2881 aattaaatgg tacagccgta cccaactgct gcgtctgcgt agctgctaca acttctatat
     2941 caccaccgat tttgcgacca gcgaaaagca agtgagcgac tacagcgtga ttagcgtttg
     3001 ggcgtatggt agcaacggcg attggttctg ggttgacggc atcgcgtgcc gtcaaaccat
     3061 ggataaaaac ttcgatgacc tgtttcgtct ggtgcaggag taccaaccgc agcaagtggg
     3121 tgttgaaacc accggccagc aaggtggctt tattagcctg ctgcagaagg agatgctgaa
     3181 ccgtaacgtg ttctttaact ttgcgagcag ccgtggtggc caaccgggta tccacccggt
     3241 taccagcaaa ctgagccgtt tcaacctggt ggttccgtgg tttaaggcgg gcaaaatgta
     3301 tttcccggcg gaaatgaagg acagcccgat tatgaccctg ttcatgggtc agatccgtct
     3361 ggcgaccatt aacggtctga agggcaaaga tgactgcatc gataccatta gcatgctggg
     3421 ttacctgaac ccgtggaaac cgcaggcggg tatggtgccg gttaacacca gcggtgaccc
     3481 gctgtgggat gatggtgatg acaccggcag cgtgaacccg ctgagcagct atatcgttta
     3541 atgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     3601 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3901 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     3961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4861 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5161 gacaggtatc cggtaagcgg cag
//
