LOCUS       sequence-369492-        5201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from Escherichia coli NCTC9020 prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_prophage_Escherichia_coli_1
FEATURES             Location/Qualifiers
     source          1..5201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     375..394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     628..645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    660..800
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(726..745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     886..908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1064..1942)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1969..2253
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2176..2195
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3918..3945
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3964..4055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4056..4124,4125..4916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4274..4293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5087..5201,1..474)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
       61 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      661 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      721 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
      781 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      841 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      901 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
      961 aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     1021 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     1081 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     1141 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     1201 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     1261 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     1321 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     1381 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     1441 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     1501 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     1561 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     1621 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     1681 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     1741 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     1801 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     1861 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     1921 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     1981 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     2041 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     2101 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     2161 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     2221 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
     2281 ttaaccatga tcagctttct ggcgttcttt ctgatgtggg cggagcgtat gaactgggac
     2341 gtgccggatt gccactaccg tgcgtgccac tggctggaac accgtggtaa cctggcggtt
     2401 ctgcgttgct tccgtggttt tggcaagagc accattctgg gcgtgtacaa cgcgtggcgt
     2461 tactattgcg accgtcagta tcgtgttctg caccaaagcg agagcgacag caccgcgcgt
     2521 aagaccagcc gtgataccca gaacgtgctg cgtaaccacc cgctgaccaa gggtatgctg
     2581 ccggacggtc tgggcaccgt ggaacaatgg tgggttaacg gcagcctgga tatgcgtaac
     2641 gcgagcatgt tcgcgaaagg tatcctgagc aacgttaccg gtgcgcgtgc gaacgagtgc
     2701 cagaacgacg atgtggaagt tccgggtaac attcaaaccc cggaggcgcg tgaaaagctg
     2761 cgttaccgtc tgagcgagca gacccacatc ctgattccgg gtggccgtaa actgtttatc
     2821 ggtaccccgc acacccacga cagcctgtat gatgagatgg aaagcgcggg tgcggactgc
     2881 ctgaccatca agctgttcga gaaggaaaaa cgtattgaag cgaaagatgc gacccaactg
     2941 cgttacgtga tcccgttccg tccggagtat gtttttgcgg gtattcacaa ggcggcgcgt
     3001 ctgctggttg agggtgtgga ctacaaactg accgcggatg gtgtggagtt tgcggcggcg
     3061 ccggaaaccg ttatcgactt ttatgcgggt tgcgcgtggc cggagcgttt tgaccacgat
     3121 gagctggaac tgcgtcgtaa ggagtgccgt accgtgaacg aatgggacag ccagtaccaa
     3181 ctgcacagca aaccggtggg tgatgttcgt ctggacccgg atcgtattcg tgagtacaac
     3241 atccacccgg aaattcgtta tgcgaaccgt accgcgagca tgtggctggg tagcacccaa
     3301 attgtgggtg cggttgcgtg gtgggatgtg gcgaccggca aggttaaagc ggatgcgagc
     3361 gctctgagcc tgattctgac cgacgcgcgt ggtcacctgt actggcacgt ttgcaaggaa
     3421 ctgaccggcg agctggcgga attcgacgat aacgataaga tcaccggtgg ccaggtggcg
     3481 caaattaaag agctggttat ccgttatcag attccggtgg tttgcgtgga agttaacggt
     3541 ccgggtggct ttgcgggcaa gctgctgcgt caggcgctga aaggtaccgg ctgcggtgtg
     3601 cgtgaggaat tcagcgttac caacaagcaa aaacgtatcc tggatgcgtt tgaggcgccg
     3661 ctgagcagcc gttttctgtg ggcgcacacc gacgtgctgg atggtccgat gtacgaccag
     3721 atgcgtgatt ttaacccggc gctgaccaac caaccggacg atttcatcga cagcggtgcg
     3781 ggtgcgatta gcgcgacccc ggttcgtatc ggcaaagtgg ttggtattcc gaccggccag
     3841 agccgtgaac actggcaact gagcgacggt gatcacgagg tggaagttga ttattaatgc
     3901 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     3961 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     4321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5161 tgccggatca agagctacca actctttttc cgaaggtaac t
//
