LOCUS       sequence-369494-        5333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage Lidtsur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_Lidtsur
FEATURES             Location/Qualifiers
     source          1..5333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     371..388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    403..543
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(469..488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     629..651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(807..1685)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1712..1996
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1919..1938
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3793..3820
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3839..3930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3931..3999,4000..4791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4149..4168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4962..5333,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
      421 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      481 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
      541 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      661 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca
      721 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
      781 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
      841 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
      901 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
      961 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     1021 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     1081 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     1141 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     1201 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     1261 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     1321 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     1381 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     1441 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     1501 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     1561 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     1621 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     1681 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     1741 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt
     1801 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     1861 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     1921 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
     1981 tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca tggtgatggc
     2041 gggtatcgtt cgtgcgcact acgagaacaa cttcccgctg tttctgcacg actatttcgc
     2101 gtggctgggc tttgacctga ccgaaatgca gctggatatt ggtcgttaca tggcggaccg
     2161 taccctgaag aacaaatgcg tgatggcgca acgtggcgag gcgaagagca ccatcggtat
     2221 tgcgcgtgcg ctgtacgaat tcgttcagat gccgaacgcg accgtgctgc tggttagcgg
     2281 cagcgacgat tatgcgggta ccctgagcca cgcgatcgtg agcgcgatta tgcaatggga
     2341 gcgtctggaa tggctgcgtc cggagacccg tgcgggtgcg cgtaccagct ataccgacgg
     2401 tttcgatgtt caccacgcgt ttcgtatccc ggataagcag ccgagcgtga aagcggttgg
     2461 cattttcggt cagctgcaag gcaaccacgt gagcctgctg atcgcggacg atgttgaaac
     2521 caccagcaac ggtagcagcc cggcgaaccg tgcgcgtatt gcgaccctga ccaaagagtt
     2581 tgcggcgatc gcgaacgacg gcgcggaaat tctgtacctg ggtaccccgc agacccaaga
     2641 tagcatctat aacagcctgc cgcaacgtgg cttcgacgtg cgtatttggc cgggtcgttt
     2701 tccgaccccg gaggaagagg aacgttacgg tggctgcctg gcgccgtgga tcaagcagaa
     2761 aatggatggt cacccggagc tgcgtaccgg ctatggtatt ctgggcaacc gtggtgcgca
     2821 gaccgatccg caacgttaca gcgaggaaaa gcagctgcaa aacgaacgtg actatcagca
     2881 agcgggcttc cagctgcaat ttatgctgga caccagcctg agcgatgcgc tgaaacagac
     2941 cctgcaactg cgtgacctga tcctgttcca cggtagcccg caaagcgcgc cggagtacct
     3001 gcactggagc aaccacccgc gtcacctggc ggaactgccg atggattttc cgctggcgcg
     3061 tgcgcagctg tacctggcgg cggactttag cgagacctat gtgccgttca ccaacaccaa
     3121 ggcgtttctg gatccggcgg gtggcggtac cgacgaaagc gttatcttcg cgaccgcgag
     3181 catcggcccg tttattcacg tgctgggtat gcacgtttat cacggcggtc agaccgagga
     3241 aaacattaag accgcggtgg agtggctgca caaaatgggc gttctgcaca tcaccattga
     3301 agataacatg ggccacggtg cggtgaccaa catgtaccgt ggcgagatca agcgtcaggg
     3361 tctggcgatt ggctgcgagg gtacctatag cagcggtcaa aaagaagtgc gtgttatcag
     3421 ccgtctgagc ccgattctgc agcgtcaccg tgtggttatg cactggagcg ttctggagga
     3481 agatctgcgt ctgtgccgta acatgcacaa cggcggtcgt gagtacagcc tgttctggca
     3541 gatgcaaaac atcacccaag acaaaaacgc gatcccgcac gacgatcgta ttgaaaccct
     3601 gagcgcggcg gtgtatctgc acgcggaagt gctgcagcaa gacgaacacg aggaagcgca
     3661 gcgtcgtgag gcgaaggcgg cgaaagaatt tctggcgaac ccgatgggct acgacgatag
     3721 caagtggcgt agccacaaac cggttcgtgg tacccgtcgt cgtatccgtt aatgccaggc
     3781 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     3841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4141 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     4201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5101 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga act
//
