LOCUS       sequence-369495-       10362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses synaptic HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Vevea JD, Chapman ER
  TITLE     Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support 
            Presynaptic Ca(2+) Homeostasis and the Synaptic Vesicle Cycle in Rat
            Hippocampal Axons.
  JOURNAL   J Neurosci. 2023 May 10;43(19):3421-3438. doi: 
            10.1523/JNEUROSCI.1356-22.2023. Epub 2023 Mar 30.
  PUBMED    36997314
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1356-22.2023"; journalName: "J Neurosci"; date: 
            "2023-05-10- 10"; volume: "43"; issue: "19"; pages: "3421-3438"
COMMENT     Sequence Label: pF(UG) hSyn SYP-HaloTag
FEATURES             Location/Qualifiers
     source          1..10362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..78)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        63..392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      243..378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     305..324
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        440..487
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             506..880
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1010..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1047..1066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1101..1120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1180..1196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1180..1196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1193..1215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1204..1220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1228..1234,1235..1252,1253..1258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1273..1294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1411..1428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1582..2170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1662..1681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2341..3132,3133..3201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2964..2983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3202..3306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3381..3400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3572..3951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3953..4151
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4106..4126
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4169..4349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4396..4521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5014..5247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5432..5476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5774..5891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5936..6382
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      6446..6455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7418..7438
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             7454..8335
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    8388..8976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8441..8461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8859..8870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8979..8995)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8980..8996)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9501..9681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9707..9724)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9713..9937
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9983..10362,1..49)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10070..10089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10280..10301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
       61 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      301 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      361 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      421 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      481 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      541 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      601 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      661 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      721 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      781 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      841 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      901 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      961 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     1021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1141 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3361 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3421 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3481 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3541 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3601 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3661 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3721 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3781 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3841 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3901 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3961 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4021 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4081 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4141 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4321 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4381 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4441 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4501 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4561 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4621 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4681 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4741 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4801 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4861 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4921 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4981 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     5041 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5101 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5161 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5221 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5281 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5341 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5401 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5461 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5521 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5581 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5641 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5701 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5761 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5821 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5881 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaaagtgc
     5941 aagtggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
     6001 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
     6061 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
     6121 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
     6181 gtcactcgcc ggtcccccgt aaactcccct tcccggccac cttggtcgcg tccgcgccgc
     6241 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
     6301 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
     6361 cgtgtcgtgc ctgagagcgc aggtacggct agcggatcgg ttcaaagttt ttttcttcca
     6421 tttcaggtgt cgtgaggaat tgcccgccac catggacgtg gtgaatcagc tggtggctgg
     6481 gggtcagttc cgggtggtca aggagcccct tggcttcgtg aaggtgctgc agtgggtctt
     6541 tgccatcttc gcctttgcta cgtgtggcag ctacaccggg gagcttcggc tgagcgtgga
     6601 gtgtgccaac aagacggaga gtgccctcaa catcgaagtt gaattcgagt accccttcag
     6661 gctgcaccaa gtgtactttg atgcaccctc ctgcgtcaaa gggggcacta ccaagatctt
     6721 cctggttggg gactactcct cgtcggctga attctttgtc accgtggctg tgtttgcctt
     6781 cctctactcc atgggggccc tggccaccta catcttcctg cagaacaagt accgagagaa
     6841 caacaaaggg cctatgatgg actttctggc tacagccgtg ttcgctttca tgtggctagt
     6901 tagttcatca gcctgggcca aaggcctgtc cgatgtgaag atggccacgg acccagagaa
     6961 cattatcaag gagatgccca tgtgccgcca gacagggaac acatgcaagg aactgaggga
     7021 ccctgtgact tcaggactca acacctcagt ggtgtttggc ttcctgaacc tggtgctctg
     7081 ggttggcaac ttatggttcg tgttcaagga gacaggctgg gcagccccat tcatgcgcgc
     7141 acctccaggc gccccggaaa agcaaccagc acctggcgat gcctacggcg atgcgggcta
     7201 cgggcagggc cccggaggct atgggcccca ggactcctac gggcctcagg gtggttatca
     7261 acccgattac gggcagccag ccagcggtgg cggtggctac gggcctcagg gcgactatgg
     7321 gcagcaaggc tatggccaac agggtgcgcc cacctccttc tccaatcaga tgaaaggatc
     7381 cggaagttcg gggtccagcg gttcttcagg cagttccgag gatctgtact ttcagagcga
     7441 taacgatgga tccgaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     7501 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     7561 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     7621 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     7681 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     7741 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     7801 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     7861 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     7921 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     7981 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     8041 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     8101 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     8161 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     8221 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     8281 cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga tttcctgagg
     8341 ccgcactcct caggtgcagg ctgcaattcg atatcaagct tatcgataat caacctctgg
     8401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8761 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8941 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     9001 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     9061 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     9121 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9181 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     9241 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     9301 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     9361 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     9421 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     9481 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9541 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9601 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9661 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     9721 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     9781 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     9841 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     9901 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     9961 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    10021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    10081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    10141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    10201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10321 tcggtctatt cttttgattt ataagggatt ttgccgattt cg
//
