LOCUS       sequence-369503-        7569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human TRAK1 with N-terminal meGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  TITLE     Mitochondrial Motor Adaptor Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: meGFP-hTRAK1
FEATURES             Location/Qualifiers
     source          1..7569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        26..329
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        330..533
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..504
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      572..581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(578..580,581..583,584..1294)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(623..644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(884..903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1231..1252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4322..4443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4359..4378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4413..4432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4450..4905)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4587..4608)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4799..4818
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4932..5036
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5038..5395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5038..5229
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5061..5081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5246..5381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5430..6224
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5430..6224
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5484..5503)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6094..6113
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6412..6431)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6456..6503
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6832..7420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7321..7340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
       61 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      121 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      181 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      241 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      301 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      361 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      421 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      481 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      541 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      661 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      781 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      841 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1021 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1081 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1261 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
     1321 ttcgaattcg atggcattgg tttttcaatt cgggcagccc gtcagggctc agcctctgcc
     1381 aggactctgc cacggcaagc tcattcggac aaacgcctgt gatgtgtgca acagcaccga
     1441 tcttccggaa gtcgagatca ttagcctgct ggaggagcag ctgccccatt ataagttaag
     1501 agccgacacc atctacggtt atgaccacga cgactggctc catacacctc tcatttctcc
     1561 agatgccaac attgacctca caaccgagca aattgaagag acgttaaaat acttcctttt
     1621 atgtgctgaa agagttggcc agatgactaa gacatataat gacatagatg ctgtcactcg
     1681 gcttcttgag gagaaagagc gggatttaga attggccgct cgcatcggcc agtcgttgtt
     1741 gaagaagaac aagaccctaa ccgagaggaa cgagctgctg gaggagcagg tggaacacat
     1801 cagggaggag gtgtctcagc tccggcatga gctgtccatg aaggatgagc tgcttcagtt
     1861 ctacaccagc gctgcggagg agagtgagcc cgagtccgtt tgctcaaccc cgttgaagag
     1921 gaatgagtcg tcctcctcag tccagaatta ctttcatttg gattctcttc aaaagaagct
     1981 gaaagacctt gaagaggaga atgttgtact tcgatccgag gccagccagc tgaagacaga
     2041 gaccatcacc tatgaggaga aggagcagca gctggtcaat gactgcgtga aggagctgag
     2101 ggatgccaat gtccagattg ctagtatctc agaggaactg gccaagaaga cggaagatgc
     2161 tgcccgccag caagaggaga tcacacacct gctatcgcaa atagttgatt tgcagaaaaa
     2221 ggcaaaagct tgcgcagtgg aaaatgaaga acttgtccag catctggggg ctgctaagga
     2281 tgcccagcgg cagctcacag ccgagctgcg tgagctggag gacaagtacg cagagtgcat
     2341 ggagatgctg catgaggcgc aggaggagct gaagaacctc cggaacaaaa ccatgcccaa
     2401 taccacgtct cggcgctacc actcgctggg cctgtttccc atggattcct tggcagcaga
     2461 gattgaggga acgatgcgca aggagctgca gttggaagag gccgagtctc cagacatcac
     2521 tcaccagaag cgtgtctttg agacagtaag aaacatcaac caggttgtca agcagagatc
     2581 tctgacccct tctcccatga acatccccgg ctccaaccag tcctcggcca tgaactccct
     2641 cctgtccagc tgcgtcagca ccccccggtc cagcttctac ggcagcgaca taggcaacgt
     2701 cgtcctcgac aacaagacca acagcatcat tctggaaaca gaggcagccg acctgggaaa
     2761 cgatgagcgg agtaagaagc cggggacgcc gggcacccca ggctcccacg acctggagac
     2821 ggcgctgagg cggctgtccc tgcgccggga gaactacctc tcggagagga ggttctttga
     2881 ggaggagcaa gagaggaagc tccaggagct ggcggagaag ggcgagctgc gcagcggctc
     2941 cctcacaccc actgagagca tcatgtccct gggcacgcac tcccgcttct ccgagttcac
     3001 cggcttctct ggcatgtcct tcagcagccg ctcctacctg cctgagaagc tccagatcgt
     3061 gaagccgctg gaaggttctg ccacacttca ccactggcag cagttggccc aacctcacct
     3121 tgggggcatc ctggaccccc ggcctggtgt ggtcaccaag ggcttccgga cgctggatgt
     3181 tgacctggac gaagtgtact gccttaacga ctttgaagaa gatgacacag gtgaccacat
     3241 ttctctccca cgcctagcta cctccactcc agttcagcac ccagagacct cagcgcacca
     3301 tcctgggaag tgcatgtctc agaccaactc caccttcacc ttcaccacct gtcgcatcct
     3361 gcatccttca gatgagctca ctcgggtcac accaagcctt aactcagccc caactccagc
     3421 ttgtggcagc accagccact tgaaatccac gccggtggcc acaccatgca ctccacggag
     3481 actgagcctg gctgagtcct tcactaacac ccgtgagtcc acgaccacca tgagcacatc
     3541 cctggggctc gtgtggctgt tgaaggagcg gggcatttct gctgccgtgt acgaccccca
     3601 gagctgggac agggccggcc ggggctccct cctgcactcc tacacgccca agatggctgt
     3661 gatcccctct actccgccga actcgcctat gcagacaccc acatcctccc caccctcctt
     3721 tgagttcaag tgcacgagcc ctccctacga caatttcctg gcttccaagc cagccagctc
     3781 catcctgagg gaagtgagag aaaagaacgt ccgcagcagc gagagccaga ccgacgtgtc
     3841 cgtctccaac ctcaacctcg tggacaaagt caggaggttt ggggtggcca aagtggtgaa
     3901 ctcagggcga gcccatgtcc ccaccttgac tgaggagcag ggacccctcc tctgtgggcc
     3961 cccggggcca gcaccagccc ttgttcccag aggcctggta cctgagggcc tgcccctcag
     4021 atgccccact gtcaccagtg ccatcggtgg gctgcagctc aatagtggca tccggcggaa
     4081 tcgcagcttc cccaccatgg tgggatctag catgcagatg aaagctcctg tgactctcac
     4141 ctcgggcatc ttgatgggtg ctaagctctc caaacaaact agcttacggt aaggatccac
     4201 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4261 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4321 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4381 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4441 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4501 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4561 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4621 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4681 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4741 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4801 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4861 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4921 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4981 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     5041 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     5401 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5461 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5521 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5581 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     5641 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5701 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5761 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5821 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5881 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5941 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6001 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6061 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6121 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6181 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6241 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6301 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6361 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     6421 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6481 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     6541 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     6601 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     6661 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     6721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7501 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     7561 ggtcattag
//
